Using dynein heavy chain 5 and creatine kinase levels in cervical fluid and blood for early diagnosing of ectopic pregnancy

2020 ◽  
Author(s):  
Cagdas Sahin ◽  
Zihni Onur Uygun ◽  
Ismet Hortu ◽  
Ali Akdemir ◽  
Meltem Kocamanoglu ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Ectopic Pregnancy ◽  
Heavy Chain ◽  
Dynein Heavy Chain

scholarly journals The Chlamydomonas Dhc1 gene encodes a dynein heavy chain subunit required for assembly of the I1 inner arm complex.

1997 ◽  
Vol 8 (4) ◽  
pp. 607-620 ◽  
Author(s):  
S H Myster ◽  
J A Knott ◽  
E O'Toole ◽  
M E Porter
Keyword(s):  
Heavy Chain ◽  
Insertional Mutagenesis ◽  
Northern Blot Analysis ◽  
Immunoblot Analysis ◽  
Transcription Unit ◽  
Peptide Sequence ◽  
Dynein Heavy Chain ◽  
The Individual ◽  
Intermediate Chains ◽  
Specific Peptide

Multiple members of the dynein heavy chain (Dhc) gene family have been recovered in several organisms, but the relationships between these sequences and the Dhc isoforms that they encode are largely unknown. To identify Dhc loci and determine the specific functions of the individual Dhc isoforms, we have screened a collection of motility mutants generated by insertional mutagenesis in Chlamydomonas. In this report, we characterize one strain, pf9-3, in which the insertion event was accompanied by a deletion of approximately 13 kb of genomic DNA within the transcription unit of the Dhc1 gene. Northern blot analysis confirms that pf9-3 is a null mutation. Biochemical and structural studies of isolated axonemes demonstrate that the pf9-3 mutant fails to assemble the I1 inner arm complex, a two-headed dynein isoform composed of two Dhcs (1 alpha and 1 beta) and three intermediate chains. To determine if the Dhc1 gene product corresponds to one of the Dhcs of the I1 complex, antibodies were generated against a Dhc1-specific peptide sequence. Immunoblot analysis reveals that the Dhc1 gene encodes the 1 alpha Dhc subunit. These studies thus, identify the first inner arm Dhc locus to be described in any organism and further demonstrate that the 1 alpha Dhc subunit plays an essential role in the assembly of the I1 inner arm complex.

Mice with a mutation in the dynein heavy chain 1 gene display sensory neuropathy but lack motor neuron disease

2009 ◽  
Vol 215 (1) ◽  
pp. 146-152 ◽  
Author(s):  
Luc Dupuis ◽  
Anissa Fergani ◽  
Kerstin E. Braunstein ◽  
Judith Eschbach ◽  
Nathalie Holl ◽  
...  
Keyword(s):  
Motor Neuron ◽  
Motor Neuron Disease ◽  
Heavy Chain ◽  
Sensory Neuropathy ◽  
Dynein Heavy Chain

Assignment1 of the human dynein heavy chain gene DNAH17L to human chromosome 17p12 by in situ hybridization and radiation hybrid mapping

1999 ◽  
Vol 84 (3-4) ◽  
pp. 188-189 ◽  
Author(s):  
L. Bartoloni ◽  
J.-L.C. Blouin ◽  
A.J. Sainsbury ◽  
A. Gos ◽  
M.A. Morris ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Chromosome ◽  
Heavy Chain ◽  
Radiation Hybrid ◽  
Radiation Hybrid Mapping ◽  
Chain Gene ◽  
Hybrid Mapping ◽  
Heavy Chain Gene ◽  
Dynein Heavy Chain

Identification and molecular evolution of new dynein-like protein sequences in rat brain

1995 ◽  
Vol 108 (5) ◽  
pp. 1883-1893 ◽  
Author(s):  
Y. Tanaka ◽  
Z. Zhang ◽  
N. Hirokawa
Keyword(s):  
Rat Brain ◽  
Heavy Chain ◽  
Phylogenetic Trees ◽  
Cytoplasmic Dynein ◽  
Amino Acid Sequences ◽  
Adult Brain ◽  
Chain Gene ◽  
Evolutionary Analysis ◽  
Degenerate Primers ◽  
Dynein Heavy Chain

RT-PCR cloning was performed to find unknown members of the dynein superfamily expressed in rat brain. Six kinds of degenerate primers designed for the dynein catalytic domain consensuses were used for extensive PCR amplifications. We have sequenced 550 plasmid clones which turned out to include 13 kinds of new dynein-like sequences (DLP1-8, 9A/B, 10–12) and cytoplasmic dynein heavy chain. In these clones, alternative splicing was detected for a 105 nt-domain containing the CFDEFNRI consensus just downstream of the most N-terminal P-loop (DLP9A and 9B). By using these obtained sequences, initial hybridization studies were performed. Genomic Southern blotting showed each sequence corresponds to a single copy of the gene, while northern blotting of adult brain presented more than one band for some subtypes. We further accomplished molecular evolutionary analysis to recognize their phylogenetic origins for the axonemal and non-axonemal (cytoplasmic) functions. Different methods (UPGMA, NJ and MP) presented well coincident phylogenetic trees from 44 partial amino acid sequences of dynein heavy chain from various eukaryotes. The trunk for all the cytoplasmic dynein heavy chain homologues diverged directly from the root of the phylogenetic tree, suggesting that the first dynein gene duplication defined two distinct functions as respective subfamilies. Of particular interest, we found a duplication event of the cytoplasmic dynein heavy chain gene giving rise to another subtype, DLP4, located between the divergence of yeast and that of Dictyostelium. Such evolutionary topology builds up an inceptive hypothesis that there are at least two non-axonemal dynein heavy chains in mammals.

scholarly journals 2M1448 Properties of human cytoplasmic dynein heavy chain 1 and 2(Molecular motor 3,The 48th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S97
Author(s):  
Ichikawa Muneyoshi ◽  
Yuta Watanabe ◽  
Takashi Murayama ◽  
Yoko Yano Toyoshima
Keyword(s):  
Annual Meeting ◽  
Heavy Chain ◽  
Molecular Motor ◽  
Cytoplasmic Dynein ◽  
Dynein Heavy Chain ◽  
Biophysical Society

Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena

2003 ◽  
pp. 017-027
Author(s):  
Gangadhara Sailaja ◽  
Leslie M. Lincoln ◽  
Jifan Chen ◽  
David J. Asai
Keyword(s):  
Gene Family ◽  
Heavy Chain ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Dynein Heavy Chain

Serum levels of smooth muscle heavy-chain myosin in patients with ectopic pregnancy

2000 ◽  
Vol 36 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Robert H. Birkhahn ◽  
Theodore J. Gaeta ◽  
Toru Suzuki ◽  
Hirohisa Katoh ◽  
Ryozo Nagai ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Ectopic Pregnancy ◽  
Heavy Chain ◽  
Serum Levels
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