Impacts of platelet‐rich fibrin and platelet‐rich plasma on primary osteoblast adhesion onto titanium implants in a bisphosphonate in vitro model

2019 ◽  
Vol 48 (10) ◽  
pp. 943-950 ◽  
Author(s):  
Daniel Steller ◽  
Nele Herbst ◽  
Ralph Pries ◽  
David Juhl ◽  
Matthias Klinger ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Platelet Rich Plasma ◽  
Titanium Implants ◽  
Platelet Rich Fibrin ◽  
Primary Osteoblast ◽  
Osteoblast Adhesion ◽  
Vitro Model

Critical Evaluation of an in Vitro Model for Evaluation of Platelet Reactivity of Biomaterials

1987 ◽  
Vol 110 ◽  
Author(s):  
Raymond Connolly ◽  
Norman Shoenfeld ◽  
Karen Ramberg ◽  
Allan D. Callow
Keyword(s):  
In Vitro Model ◽  
Platelet Reactivity ◽  
Platelet Rich Plasma ◽  
Critical Evaluation ◽  
The Body ◽  
Test Material ◽  
In Vitro Test ◽  
Vitro Model

AbstractAn in vitro model for measuring platelet reactivity to a variety of biomaterial candidates for vascular grafts is described. A model consisting of a standard area of test material exposed to freshly labeled In platelets in plasma was evaluated. The platelets were isolated from ACD anticoagulated blood and resuspended in ACD plasma. It has been previously demonstrated that platelets so treated circulate in the body and will deposit on biomaterials exposed to the blood in vivo. The in vitro test consisted of an incubation of the platelets and materials at 37°C for one hour. At the end of the incubation, the platelet rich plasma was removed and the materials washed and removed for gamma counting. Platelet reactivity was normalized as a percentage of the counts on the material to counts in an aliquot of the platelet-plasma incubation media. The maximum uptake of platelets occurred within one hour. Platelets from three species, human, baboon, and dog were tested. Platelet uptake by Dacron and PTFE were in the range of 30–40% and 1–5% respectively. This is in accord with the known reactivity of these two vascular graft materials in vivo.A second series of studies were conducted with physically and pharmacologically inactivated platelets and inert particles. Those studies suggest that the initial results do not represent a biologic event but may reflect the porosity of the materials. This emphasizes the necessity of adequately defining an in vitro model against known in vivo activity.

Platelet‐rich plasma enhances the integration of bioengineered cartilage with native tissue in an in vitro model

2017 ◽  
Vol 12 (2) ◽  
pp. 427-436 ◽  
Author(s):  
Corey Sermer ◽  
Rita Kandel ◽  
Jesse Anderson ◽  
Mark Hurtig ◽  
John Theodoropoulos
Keyword(s):  
In Vitro Model ◽  
Platelet Rich Plasma ◽  
Native Tissue ◽  
Vitro Model

scholarly journals Platelet-rich plasma plays an antibacterial, anti-inflammatory and cell proliferation-promoting role in an in vitro model for diabetic infected wounds

2019 ◽  
Vol Volume 12 ◽  
pp. 297-309 ◽  
Author(s):  
Tao Li ◽  
Yu Ma ◽  
Min Wang ◽  
Tao Wang ◽  
Jing Wei ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
In Vitro Model ◽  
Platelet Rich Plasma ◽  
Infected Wounds ◽  
Anti Inflammatory ◽  
Vitro Model

Effects of pulsed electromagnetic fields and platelet rich plasma in preventing osteoclastogenesis in an in vitro model of osteolysis

2017 ◽  
Vol 233 (3) ◽  
pp. 2645-2656 ◽  
Author(s):  
Matilde Tschon ◽  
Francesca Veronesi ◽  
Deyanira Contartese ◽  
Maria Sartori ◽  
Lucia Martini ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
In Vitro Model ◽  
Platelet Rich Plasma ◽  
Pulsed Electromagnetic Fields ◽  
Vitro Model ◽  
Pulsed Electromagnetic

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Präsidentenposter: Regulation der Expression der β-Defensine hBD-2 und hBD-3 in einem in vitro Model der Candida Ösophagitis: NF-κB, MAPK/AP-1 und EGFR

2009 ◽  
Vol 47 (09) ◽  
Author(s):  
N Steubesand ◽  
K Kiehne ◽  
R Pahl ◽  
G Brunke ◽  
UR Fölsch ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Vitro Model

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model

A novel in vitro model for joint effects of alcohol and free fatty acids on hepatocellular steatosis and inflammation

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
A Mahli ◽  
WE Thasler ◽  
M Müller ◽  
C Hellerbrand
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
In Vitro Model ◽  
Joint Effects ◽  
Vitro Model
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