scholarly journals Effect of feline characteristics on plasma N‐terminal‐prohormone B‐type natriuretic peptide concentration and comparison of a point‐of‐care test and an ELISA test

2020 ◽  
Vol 34 (3) ◽  
pp. 1187-1197
Author(s):  
Sofia Hanås ◽  
Bodil S. Holst ◽  
Katja Höglund ◽  
Jens Häggström ◽  
Anna Tidholm ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Point Of Care ◽  
Elisa Test ◽  
Peptide Concentration ◽  
Point Of Care Test

scholarly journals Sero-prevalence and Risk Factors for Hepatitis A Virus Infection among Pregnant Women at the Samandin Medical Center, Ouagadougou, Burkina Faso

2019 ◽  
pp. 1-9
Author(s):  
Rouamba Poda Somda Hortense ◽  
Ouoba Jean Bienvenue ◽  
Traoré Kuan Abdoulaye ◽  
Ouoba Bruno Lalidia ◽  
Kagambéga Asséta ◽  
...  
Keyword(s):  
Risk Factors ◽  
Burkina Faso ◽  
Pregnant Women ◽  
Hepatitis A Virus ◽  
Medical Center ◽  
Point Of Care ◽  
Epidemiological Survey ◽  
Elisa Test ◽  
Cross Sectional ◽  
Point Of Care Test

Aims: This study determined prevalence and possible risk factors associated with HAV infection and compared Immunochromatography (IgG) rapid point of care test with standard ELISA test for diagnosis. Study Design:  Cross sectional epidemiological survey. Place and duration of Study: The study was carried out at the Samandin Medical Center (Ouagadougou, Burkina Faso), from July 2017 to December 2017. Methodology: A total of 180 pregnant women were recruited at the Samandin Medical Center. Anti-HAV IgM and IgG detection tests were performed using AccuDiag™ HAV ELISA kit (Calabasas, CA, USA) and SD BIOLINE HAV IgG/IgM Rapid Diagnostic Test (Gyeonggi-do, Korea). Results: IgG anti-HAV antibodies (AccuDiag Elisa) were found with a prevalence of 88.9% (160/180) and Anti-HAV IgM were 1.1% (2/180). Of the 160 AccuDiag HAV IgG positive samples, 44.4% (80/180) tested positive using SD BIOLINE Diagnostics HAV IgG. Regarding risk factors, 86.9% (86/99) of the IgG positive women were illiterate; 90.7% (39/43) lived mostly in peripheral areas; 89.2% (74/83) were household workers and 88.6% (93/105) used fountain water. Conclusion: Our results demonstrated poor agreement between the assays tested, which are consistent with previous reports demonstrating significant variability between HAV ELISA and RDT HAV. Moreover, the detection of HAV specific IgM antibodies in two asymptomatic pregnant women signaled the current circulation of HAV in this area.

scholarly journals Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes

2017 ◽  
Vol 1 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Prima Dewi Sinawang ◽  
Dorin Harpaz ◽  
Luka Fajs ◽  
Raymond Chee Seong Seet ◽  
Alfred Iing Yoong Tok ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Point Of Care ◽  
Cardioembolic Stroke ◽  
Gold Electrodes ◽  
Plasma Samples ◽  
Label Free ◽  
Point Of Care Test ◽  
Novel Approach ◽  
Set Up ◽  
Screen Printed

Abstract Stroke is the second top leading cause of death globally. It is caused by an abrupt interruption of blood flow to the brain. In that course, brain natriuretic peptide (BNP) and its derivative N-terminal pro b-type natriuretic peptide (NT-proBNP), neurohormones produced mainly by the heart ventricles in response to excessive stretching of cardiomyocytes (heart muscle cells), are proven to be good biomarkers for heart failure diagnosis. Moreover, there is growing clinical interest of the use of NT-proBNP for stroke diagnosis and prognosis because it is significantly associated with cardioembolic stroke and secondary stroke reoccurrence, with sensitivity >90% and specificity >80%. However, in diagnostic settings, there is still a need to address the encountered analytical problems, particularly assay specificity and set up. In this study, a novel approach for NT-proBNP detection is demonstrated using an electrochemical immunoassay method. A label-free impedimetry immunosensor for stroke biomarker was developed using modified disposable screen-printed gold electrodes (SPGE) hosting specific anti-NT-proBNP capture antibody. The performance of our immunosensor was studied in the presence of NT-proBNP in both buffered and mock (porcine) plasma samples. A linear relation between the relative total resistance (ΔRtot) responses and the NT-proBNP concentrations in buffer was observed in a range from 0.1 to 5 ng mL-1 with a correlation coefficient (R2) of 0.94656. Overall, the biosensor has demonstrated the capability to quantitate NT-proBNP and differentiate such concentrations in a low concentration range, especially among 0, 0.1, 0.5, 1, and 3 ng mL-1 in plasma samples within 25 min. This range is valuable not only for classifying cardioembolic stroke (higher or equal to 0.5 ng mL-1), but also predicting the risk of secondary stroke reoccurrence (higher than 0.255 ng mL-1). Our biosensor has the potential to be used as an easy-to-use point-of-care test that is both accurate and affordable.

scholarly journals Use of B-type natriuretic peptide (BNP) as a rapid, point-of-care test for screening patients undergoing echocardiography for ventricular dysfunction

2000 ◽  
Vol 2 ◽  
pp. 90-90
Author(s):  
Jen Koon ◽  
Padma Krishnaswamy ◽  
Radmilia Casengra ◽  
Nancy Gardetto ◽  
Anthony DeMaria ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Ventricular Dysfunction ◽  
Point Of Care ◽  
Point Of Care Test

Testing for resistance: Point-of-care testing as a communicational tool in antibiotic prescribing

2018 ◽  
Vol 14 (3) ◽  
pp. 229-240
Author(s):  
Johanna Lindell
Keyword(s):  
Point Of Care ◽  
Rapid Test ◽  
Antibiotic Use ◽  
Treatment Recommendation ◽  
Antibiotic Prescribing ◽  
Communicative Practices ◽  
Effective Strategies ◽  
Reactive Protein ◽  
Point Of Care Test ◽  
Test Result

As antibiotic resistance becomes a growing health emergency, effective strategies are needed to reduce inappropriate antibiotic use. In this article, one such strategy – communicative practices associated with the C-reactive protein point-of care test – is investigated. Building on a collection of 31 videorecorded consultations from Danish primary care, and using conversation analysis, this study finds that the rapid test can be used throughout the consultation to incrementally build the case for a nonantibiotic treatment recommendation, both when the test result is forecast and reported. The study also finds that the format of reports of elevated results differs from that of ‘normal’ results, resulting in a subtle shift of authority from doctor to test.

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