scholarly journals The utility of the PCR melting profile technique for typingCorynebacterium diphtheriaeisolates

2014 ◽  
Vol 59 (3) ◽  
pp. 292-298 ◽  
Author(s):  
A. A. Zasada ◽  
K. Formińska ◽  
T. Wołkowicz ◽  
E. Badell ◽  
N. Guiso
Keyword(s):  
Melting Profile ◽  
Profile Technique

scholarly journals Evaluation of a PCR Melting Profile Technique for Bacterial Strain Differentiation

2006 ◽  
Vol 44 (7) ◽  
pp. 2327-2332 ◽  
Author(s):  
B. Krawczyk ◽  
A. Samet ◽  
J. Leibner ◽  
A. Sledzinska ◽  
J. Kur
Keyword(s):  
Bacterial Strain ◽  
Melting Profile ◽  
Strain Differentiation ◽  
Profile Technique

scholarly journals Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

2003 ◽  
Vol 69 (4) ◽  
pp. 2377-2382 ◽  
Author(s):  
Said El Fantroussi ◽  
Hidetoshi Urakawa ◽  
Anne E. Bernhard ◽  
John J. Kelly ◽  
Peter A. Noble ◽  
...  
Keyword(s):  
Thermal Dissociation ◽  
Pcr Amplification ◽  
Estuarine Sediments ◽  
Microbial Populations ◽  
Oligonucleotide Microarrays ◽  
Dissociation Curve ◽  
Melting Profile ◽  
Probe Target ◽  
Study Sites

ABSTRACT Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

Development of an Instantaneous Velocity-Vector-Profile Method Using Conventional Ultrasonic Transducers

2021 ◽  
Author(s):  
Dongik Yoon ◽  
Hyun Jin Park ◽  
Tomonori Ihara
Keyword(s):  
Velocity Vector ◽  
Ultrasound Velocity ◽  
Ultrasonic Transducers ◽  
New Method ◽  
Ultrasound Transducers ◽  
Accuracy And Precision ◽  
Conventional Ultrasound ◽  
Measurable Distance ◽  
Profile Technique ◽  
Good Agreement

Abstract The velocity vector profile technique based on an ultrasound pulsed Doppler method can enrich the information of a flow field, however, it has shown a low availability because a new design of special transducers is required for each measurement case. This study proposes a new method of profiling the velocity vectors using conventional ultrasound transducers that are widely supplied to UVP (Ultrasound velocity profile) users. We constructed a configuration of the transducers to minimize the uncertainty of the detection points at the receivers, and a measurable distance was theoretically determined by the configuration. Two feasibility tests were carried out. One was a test for the assessment of the measurable distance, which agreed well with the theoretical distance. The other was the evaluation of the measurement of two-dimensional velocity vectors by the new method and it was performed in a towing tank facility without the velocity fluctuation. From the evaluation, it was confirmed that the measured vectors showed good agreement to the reference values, and their accuracy and precision were competitive compared to previous studies. The developed method was applied to two unsteady flows for demonstrations. The results clarified that the proposed method guarantees high availability and accuracy for the velocity vector profiles.

Genes Leave Their Characteristic Marks on the Melting Profile of Plasmid DNAs

1995 ◽  
Vol 50 (9-10) ◽  
pp. 652-655 ◽  
Author(s):  
Igor Z. Zubrzycki ◽  
Horst H. Klump
Keyword(s):  
High Resolution ◽  
Resistance Gene ◽  
High Resolution Melting ◽  
Uv Absorbance ◽  
Melting Profile ◽  
Plasmid Dnas ◽  
Parental Plasmid

Abstract High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. teperature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a charactersitic mark on the melting profile of its parental plasmid

scholarly journals A mantle melting profile across the Basin and Range, SW USA

2002 ◽  
Vol 107 (B1) ◽  
pp. ECV 5-1-ECV 5-21 ◽  
Author(s):  
K. Wang ◽  
T. Plank ◽  
J. D. Walker ◽  
E. I. Smith
Keyword(s):  
Basin And Range ◽  
Mantle Melting ◽  
Melting Profile

A high-resolution NMR and synchrotron x-ray powder diffraction study of zeolite ZSM-11

1988 ◽  
Vol 3 (3) ◽  
pp. 563-569 ◽  
Author(s):  
B. H. Toby ◽  
M. M. Eddy ◽  
C. A. Fyfe ◽  
G. T. Kokotailo ◽  
H. Strobl ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Powder Diffraction ◽  
Nmr Spectra ◽  
Powder Diffraction Data ◽  
X Ray ◽  
Modeling Methods ◽  
High Resolution Nmr ◽  
Profile Technique

High-resolution nuclear magnetic resonance (NMR) spectra and synchrotron x-ray powder diffraction data have been obtained from a well-crystallized highly dealuminated sample of the zeolite ZSM-11. The Rietveld profile technique has been applied to the synchrotron data to give the first detailed refinement of the idealized structure derived ten years ago by distance least-squares modeling methods [G. T. Kokotailo, P. Chu, S. L. Lawton, and W. M. Meier, Nature 275, 119 (1978)], which involves 54 variable atomic positional parameters. The structure is tetragonal (a = 20.065 Å, c = 13.408 Å at 25 °C) and consistent with the previously reported tetragonal space group I \overline 4 m2, but the NMR spectra indicate local deviations from this symmetry that disappear at 100 °C.

Sign in / Sign up

Export Citation Format

Share Document