Improving riboflavin production by modifying related metabolic pathways in Bacillus subtilis

2021 ◽  
Author(s):  
J. Xu ◽  
C. Wang ◽  
R. Ban
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Pathways ◽  
Riboflavin Production

scholarly journals Proteomic responses of spores of Bacillus subtilis to thermosonication involve large-scale alterations in metabolic pathways

2020 ◽  
Vol 64 ◽  
pp. 104992
Author(s):  
Lihua Fan ◽  
Furong Hou ◽  
Aliyu Idris Muhammad ◽  
Balarabe Bilyaminu Ismail ◽  
Ruiling lv ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Pathways ◽  
Large Scale ◽  
Proteomic Responses

scholarly journals Design of a programmable biosensor-CRISPRi genetic circuits for dynamic and autonomous dual-control of metabolic flux in Bacillus subtilis

2019 ◽  
Vol 48 (2) ◽  
pp. 996-1009 ◽  
Author(s):  
Yaokang Wu ◽  
Taichi Chen ◽  
Yanfeng Liu ◽  
Rongzhen Tian ◽  
Xueqin Lv ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Pathways ◽  
Metabolic Flux ◽  
Target Genes ◽  
Fine Tuning ◽  
Dual Control ◽  
Genetic Circuits ◽  
Dynamic Regulation ◽  
Batch Bioreactor ◽  
Acetoin Production

Abstract Dynamic regulation is an effective strategy for fine-tuning metabolic pathways in order to maximize target product synthesis. However, achieving dynamic and autonomous up- and down-regulation of the metabolic modules of interest simultaneously, still remains a great challenge. In this work, we created an autonomous dual-control (ADC) system, by combining CRISPRi-based NOT gates with novel biosensors of a key metabolite in the pathway of interest. By sensing the levels of the intermediate glucosamine-6-phosphate (GlcN6P) and self-adjusting the expression levels of the target genes accordingly with the GlcN6P biosensor and ADC system enabled feedback circuits, the metabolic flux towards the production of the high value nutraceutical N-acetylglucosamine (GlcNAc) could be balanced and optimized in Bacillus subtilis. As a result, the GlcNAc titer in a 15-l fed-batch bioreactor increased from 59.9 g/l to 97.1 g/l with acetoin production and 81.7 g/l to 131.6 g/l without acetoin production, indicating the robustness and stability of the synthetic circuits in a large bioreactor system. Remarkably, this self-regulatory methodology does not require any external level of control such as the use of inducer molecules or switching fermentation/environmental conditions. Moreover, the proposed programmable genetic circuits may be expanded to engineer other microbial cells and metabolic pathways.

Rewiring the Glucose Transportation and Central Metabolic Pathways for Overproduction of N ‐Acetylglucosamine in Bacillus subtilis

2017 ◽  
Vol 12 (10) ◽  
pp. 1700020 ◽  
Author(s):  
Yang Gu ◽  
Jieying Deng ◽  
Yanfeng Liu ◽  
Jianghua Li ◽  
Hyun‐dong Shin ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Pathways

scholarly journals Clp-Dependent Proteolysis Down-Regulates Central Metabolic Pathways in Glucose-Starved Bacillus subtilis

2007 ◽  
Vol 190 (1) ◽  
pp. 321-331 ◽  
Author(s):  
Ulf Gerth ◽  
Holger Kock ◽  
Ilja Kusters ◽  
Stephan Michalik ◽  
Robert L. Switzer ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Metabolic Pathways ◽  
Time Course ◽  
Polyacrylamide Gel Electrophoresis ◽  
Nucleotide Metabolism ◽  
Clp Proteases ◽  
In The Wild ◽  
Branched Chain ◽  
Expression Program ◽  
Intracellular Proteolysis

ABSTRACT Entry into stationary phase in Bacillus subtilis is linked not only to a redirection of the gene expression program but also to posttranslational events such as protein degradation. Using 35S-labeled methionine pulse-chase labeling and two-dimensional polyacrylamide gel electrophoresis we monitored the intracellular proteolysis pattern during glucose starvation. Approximately 200 protein spots diminished in the wild-type cells during an 8-h time course. The degradation rate of at least 80 proteins was significantly reduced in clpP, clpC, and clpX mutant strains. Enzymes of amino acid and nucleotide metabolism were overrepresented among these Clp substrate candidates. Notably, several first-committed-step enzymes for biosynthesis of aromatic and branched-chain amino acids, cell wall precursors, purines, and pyrimidines appeared as putative Clp substrates. Radioimmunoprecipitation demonstrated GlmS, IlvB, PurF, and PyrB to be novel ClpCP targets. Our data imply that Clp proteases down-regulate central metabolic pathways upon entry into a nongrowing state and thus contribute to the adaptation to nutrient starvation. Proteins that are obviously nonfunctional, unprotected, or even “unemployed” seem to be recognized and proteolyzed by Clp proteases when the resources for growth become limited.

scholarly journals Bacillus subtilis Homologs of MviN (MurJ), the Putative Escherichia coli Lipid II Flippase, Are Not Essential for Growth

2009 ◽  
Vol 191 (19) ◽  
pp. 6020-6028 ◽  
Author(s):  
Allison Fay ◽  
Jonathan Dworkin
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Metabolic Pathways ◽  
Growth Defect ◽  
Membrane Translocation ◽  
E Coli ◽  
Peptidoglycan Synthesis ◽  
Lipid Ii

ABSTRACT Although peptidoglycan synthesis is one of the best-studied metabolic pathways in bacteria, the mechanism underlying the membrane translocation of lipid II, the undecaprenyl-disaccharide pentapeptide peptidoglycan precursor, remains mysterious. Recently, it was proposed that the essential Escherichia coli mviN gene encodes the lipid II flippase. Bacillus subtilis contains four proteins that are putatively homologous to MviN, including SpoVB, previously reported to be necessary for spore cortex peptidoglycan synthesis during sporulation. MviN complemented the sporulation defect of a ΔspoVB mutation, and SpoVB and another of the B. subtilis homologs, YtgP, complemented the growth defect of an E. coli strain depleted for MviN. Thus, these B. subtilis proteins are likely to be MviN homologs. However, B. subtilis strains lacking these four proteins have no defects in growth, indicating that they likely do not serve as lipid II flippases in this organism.

scholarly journals Scale-down and parallel operation of the riboflavin production process with Bacillus subtilis

2007 ◽  
Vol 33 (3) ◽  
pp. 263-274 ◽  
Author(s):  
Bettina Knorr ◽  
Heinrich Schlieker ◽  
Hans-Peter Hohmann ◽  
Dirk Weuster-Botz
Keyword(s):  
Bacillus Subtilis ◽  
Production Process ◽  
Parallel Operation ◽  
Riboflavin Production ◽  
Scale Down
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