Polymorphism pattern at a miniature inverted-repeat transposable element locus downstream of the domestication geneTeosinte-branched1in wild and domesticated pearl millet

2012 ◽  
Vol 22 (2) ◽  
pp. 327-340 ◽  
Author(s):  
Y. Dussert ◽  
M.-S. Remigereau ◽  
M. C. Fontaine ◽  
A. Snirc ◽  
G. Lakis ◽  
...  

2019 ◽  
Vol 98 (4) ◽  
pp. 639-653 ◽  
Author(s):  
Yanyan Tang ◽  
Xin Ma ◽  
Shuangshuang Zhao ◽  
Wei Xue ◽  
Xu Zheng ◽  
...  


Crop Science ◽  
2011 ◽  
Vol 51 (2) ◽  
pp. 854-861 ◽  
Author(s):  
Keenan Amundsen ◽  
David Rotter ◽  
Huaijun Michael Li ◽  
Joachim Messing ◽  
Geunhwa Jung ◽  
...  


PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e94499 ◽  
Author(s):  
Perumal Sampath ◽  
Jayakodi Murukarthick ◽  
Nur Kholilatul Izzah ◽  
Jonghoon Lee ◽  
Hong-Il Choi ◽  
...  




2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Jianqiang Shen ◽  
Juhong Liu ◽  
Kabin Xie ◽  
Feng Xing ◽  
Fang Xiong ◽  
...  


Genetica ◽  
2006 ◽  
Vol 128 (1-3) ◽  
pp. 205-216 ◽  
Author(s):  
Marie-Stanislas Remigereau ◽  
Odile Robin ◽  
Sonia Siljak-Yakovlev ◽  
Aboubakry Sarr ◽  
Thierry Robert ◽  
...  


Genetics ◽  
1995 ◽  
Vol 139 (3) ◽  
pp. 1411-1419
Author(s):  
G A Huttley ◽  
A F MacRae ◽  
M T Clegg

Abstract We report an Ac-like sequence from pearl millet (Pennisetum glaucum) and deletion derivative Ac-like sequences from pearl millet and another grass species, Bambusa multiplex. Sequence relationships between the pearl millet and maize Ac elements suggest that Ac/Ds transposable-element family is ancient. Further, the sequence identity between the Bambusa Ac-like sequence and maize Ac implies that the Ac/Ds transposable-element family has been in the grass family since its inception. The Ac-like sequences reported from pearl millet and maize Ac are statistically heterogeneous in pair-wise distance comparisons to each other. Yet, we are unable to discriminate between differential selection or ectopic exchange (recombination and conversion) between nonidentical transposable element homologues, as the cause of the heterogeneity. However, the more extreme heterogeneity exhibited between the previously described pearl millet element and maize Ac seems likely to derive from ectopic exchange between elements with different levels of divergence.



mSphere ◽  
2017 ◽  
Vol 2 (3) ◽  
Author(s):  
Dae-Wi Kim ◽  
Cung Nawl Thawng ◽  
Sang Hee Lee ◽  
Chang-Jun Cha

ABSTRACT In the present study, plasmid pAC3 isolated from a highly fluoroquinolone-resistant isolate of Aeromonas species was sequenced and found to contain two fluoroquinolone resistance genes, aac(6′)-Ib-cr and qnrS2. Comparative analyses of plasmid pAC3 and other Aeromonas sp. IncU-type plasmids revealed a mobile insertion cassette element with a unique structure containing a qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure. This study also revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Our results also demonstrate that the fluoroquinolone-dependent expression of qnrS2 is associated with rsd in E. coli DH5α harboring plasmid pAC3. Our findings suggest that the mobile element may play an important role in qnrS2 dissemination and that Aeromonas species constitute an important reservoir of fluoroquinolone resistance determinants in the environment. A highly fluoroquinolone-resistant isolate of Aeromonas species was isolated from a wastewater treatment plant and found to possess multiple resistance mechanisms, including mutations in gyrA and parC, efflux pumps, and plasmid-mediated quinolone resistance (PMQR) genes. Complete sequencing of the IncU-type plasmid, pAC3, present in the strain revealed a circular plasmid DNA 15,872 bp long containing two PMQR genes [qnrS2 and aac(6′)-Ib-cr]. A mobile insertion cassette element containing the qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure was identified in the plasmid. The present study revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Plasmid pAC3 was introduced into Escherichia coli, and its PMQR genes were expressed, resulting in the acquisition of resistance. Proteome analysis of the recipient E. coli strain harboring the plasmid revealed that aac(6′)-Ib-cr expression was constitutive and that qnrS2 expression was dependent upon fluoroquinolone stress through regulation by regulator of sigma D (Rsd). To the best of our knowledge, this is the first report to characterize a novel MITE sequence upstream of the PMQR gene within a mobile insertion cassette, as well as the regulation of qnrS2 expression. Our results suggest that this mobile element may play an important role in qnrS2 dissemination. IMPORTANCE In the present study, plasmid pAC3 isolated from a highly fluoroquinolone-resistant isolate of Aeromonas species was sequenced and found to contain two fluoroquinolone resistance genes, aac(6′)-Ib-cr and qnrS2. Comparative analyses of plasmid pAC3 and other Aeromonas sp. IncU-type plasmids revealed a mobile insertion cassette element with a unique structure containing a qnrS2 gene and a typical miniature inverted-repeat transposable element (MITE) structure. This study also revealed that this MITE sequence appears in other Aeromonas species plasmids and chromosomes. Our results also demonstrate that the fluoroquinolone-dependent expression of qnrS2 is associated with rsd in E. coli DH5α harboring plasmid pAC3. Our findings suggest that the mobile element may play an important role in qnrS2 dissemination and that Aeromonas species constitute an important reservoir of fluoroquinolone resistance determinants in the environment.



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