Electrogram recording and analyzing techniques to optimize selection of target sites for ablation of cardiac arrhythmias

Abstract Background: Sleeping Beauty (SB) transposon had been thought to strictly integrate into TA dinucleotides. Recently, we found that SB also integrates into non-TA sites at a lower frequency. Here we performed further study on the non-TA integration of SB. Results: 1) SB can integrate into non-TA sites in HEK293T cells as well as in mouse cell lines. 2) Both the hyperactive transposase SB100X and the traditional SB11 catalyze integrations at non-TA sites. 3) The consensus sequence of the non-TA target sites only occur at the opposite side of the sequenced junction between transposon end and the genomic sequences, indicating that the integrations at non-TA sites are mainly aberrant integrations. 4) The consensus sequence of the non-TA target sites is corresponding to the transposon end sequence. When the transposon end sequence is mutated, the consensus sequences changed too. Conclusion: The interaction between the SB transposon end and genomic DNA may be involved in the target site selection of the SB integrations at non-TA sites.

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Determination of interactions between structured nucleic acids by fluorescence resonance energy transfer (FRET): selection of target sites for functional nucleic acids

Nucleic Acids Research ◽

10.1093/nar/26.3.735 ◽

1998 ◽

Vol 26 (3) ◽

pp. 735-743 ◽

Cited By ~ 26

Author(s):

N Ota

Keyword(s):

Energy Transfer ◽

Nucleic Acids ◽

Fluorescence Resonance Energy Transfer ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Target Sites ◽

Fluorescence Resonance ◽

Functional Nucleic Acids ◽

Selection Of

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Seasat over-land scatterometer data. II. Selection of extended area and land-target sites for the calibration of spaceborne scatterometers

IEEE Transactions on Geoscience and Remote Sensing ◽

10.1109/36.35967 ◽

1989 ◽

Vol 27 (6) ◽

pp. 779-788 ◽

Cited By ~ 37

Author(s):

R.G. Kennett ◽

F.K. Li

Keyword(s):

Target Sites ◽

Extended Area ◽

Selection Of

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Radiofrequency Catheter Ablation of Ventricular Tachycardia in Patients with Structural Heart Disease: Pathophysiologic Considerations to Simplify the Selection of the Target Sites

Cardiac Arrhythmias 1995 ◽

10.1007/978-88-470-2223-2_10 ◽

1996 ◽

pp. 55-64

Author(s):

J. Farré ◽

J. M. Rubio ◽

A. Negrete ◽

M. Nogueira ◽

J. Romero

Keyword(s):

Heart Disease ◽

Ventricular Tachycardia ◽

Catheter Ablation ◽

Radiofrequency Catheter Ablation ◽

Structural Heart Disease ◽

Target Sites ◽

Selection Of

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Antiarrhythmic Drug Therapy: Understanding Options for the Ablationist

Mayo Clinic Electrophysiology Manual ◽

10.1093/med/9780199941193.003.0008 ◽

2013 ◽

pp. 191-202

Author(s):

Arshad Jahangir

Keyword(s):

Cardiac Arrhythmias ◽

Drug Disposition ◽

Antiarrhythmic Agents ◽

Antiarrhythmic Drug Therapy ◽

Safe Medication ◽

Arrhythmia Mechanisms ◽

Disease Condition ◽

Prognostic Implications ◽

Arrhythmia Prevention ◽

Selection Of

Antiarrhythmic agents play an important role in the management of cardiac arrhythmias as both primary and hybrid therapy. The essential goals of antiarrhythmic therapy are termination of an ongoing arrhythmia, prevention of arrhythmia recurrence, or both. Antiarrhythmic drugs help control arrhythmias but also may cause them. Therefore, selection of an effective yet safe medication may be challenging. The challenge arises from factors intrinsic to the patient, the disease condition, or the drug itself. These factors include variability in the pathophysiologic substrate, arrhythmia mechanisms, clinical presentation, prognostic implications, drug disposition, and response. Patients with arrhythmia comprise a highly heterogeneous patient population, with variable comorbid conditions and concomitant drug use.

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Transient outward potassium channel regulation in healthy and diabetic heartsThis article is one of a selection of papers from the NATO Advanced Research Workshop on Translational Knowledge for Heart Health (published in part 1 of a 2-part Special Issue).

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y08-106 ◽

2009 ◽

Vol 87 (2) ◽

pp. 77-83 ◽

Cited By ~ 13

Author(s):

Mónica Gallego ◽

Aintzane Alday ◽

Janire Urrutia ◽

Oscar Casis

Keyword(s):

Cardiac Arrhythmias ◽

Functional Expression ◽

Trophic Factors ◽

Diabetic Patients ◽

Special Issue ◽

Channel Regulation ◽

Research Workshop ◽

Sympathetic Nervous ◽

Selection Of

Diabetic patients have a higher incidence of cardiac arrhythmias, including ventricular fibrillation and sudden death, and show important alterations in the electrocardiogram, most of these related to the repolarization. In myocytes isolated from diabetic hearts, the transient outward K+ current (Ito) is the repolarizing current that is mainly affected. Type 1 diabetes alters Ito at 3 levels: the recovery of inactivation, the responsiveness to physiologic regulators, and the functional expression of the channel. Diabetes slows down Ito recovery of inactivation because it triggers the switching from fast-recovering Kv4.x channels to the slow-recovering Kv1.4. Diabetic animals also have decreased responsiveness of Ito towards the sympathetic nervous system; thus, the diabetic heart develops a resistance to its physiologic regulator. Finally, diabetes impairs support of various trophic factors required for the functional expression of the channel and reduces Ito amplitude by decreasing the amount of Kv4.2 and Kv4.3 proteins.

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