Effect of Different Energy Densities of 915 nm Low Power Laser on The Biological Behavior of Human Gingival Fibroblast Cells in‐vitro

2021 ◽  
Author(s):  
Saeed Sadatmansouri ◽  
Bahar Agahikesheh ◽  
Mohammadreza Karimi ◽  
Ardavan Etemadi ◽  
Sogol Saberi
Keyword(s):  
Low Power ◽  
Biological Behavior ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Power Laser

scholarly journals In vitro evaluation of Eugenia dysenterica in primary culture of human gingival fibroblast cells

2019 ◽  
Vol 33 ◽  
Author(s):  
Cláudio Rodrigues Rezende Costa ◽  
Bruna Rabelo Amorim ◽  
Sandra Márcia Mazutti da Silva ◽  
Ana Carolina Acevedo ◽  
Pérola de Oliveira Magalhães ◽  
...  
Keyword(s):  
Primary Culture ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Evaluation ◽  
Eugenia Dysenterica

The Application of a Recombinant Antimicrobial Peptide of Thrombocidin-1 expressed in Pichia pastoris as a Novel Approach Against Some Oral Pathogenic Bacteria: An In-vitro Study

2021 ◽  
Vol 28 ◽  
Author(s):  
Fatemeh Forouzanfar ◽  
Hamideh Sadat Mohammadipour ◽  
Majid Akbari ◽  
Reza Beyraghshamshir ◽  
Abbas Tanhaeian ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Dilution Method ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Oral Infections ◽  
Oral Pathogens

Objective: Oral infections and dental caries are considered serious health problems. Therefore, searching for new agents with antimicrobial properties seems to be crucial. This study aimed to evaluate the antimicrobial activity of the recombinant Thrombocidin-1 [TC-1] peptide on some oral pathogens. Also, the cytotoxicity of this peptide on human gingival fibroblast cells was investigated. Methods & Materials: In this study, Pichia pastoris was used for the expression of recombinant TC-1. The microbroth dilution method was used to determine the minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC]. It tested against four main oral pathogens; Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, and Enterococcus faecalis. Moreover, the cytotoxicity analysis was done on gingival fibroblast cells by the MTT method. The data were analyzed using a two-way analysis of variance [ANOVA] and Tukey’s HSD tests. Results: The most bactericidal effect of TC-1 was against S. salivarius, the highest bacteriostatic effect was against S. salivarius, and S. oralis had the lowest MIC value of 1.512 μg/ml. The Thrombocidin-1 peptide showed lower antibacterial properties against E. faecalis compared with CHX, unlike the stronger antimicrobial effect on examined streptococci. According to cytotoxicity examination, no concentration of TC-1 presented over 50% growth inhibition [IC50] of the fibroblasts cells. Conclusion: Based on antimicrobial tests and cytotoxicity results, the Thrombocidin-1 peptide may be useful as a safe antibacterial agent against some oral pathogens in dental materials.

In vitro effects of ascorbic acid and β-glycerophosphate on human gingival fibroblast cells

2012 ◽  
Vol 44 (5) ◽  
pp. 325-331 ◽  
Author(s):  
Elizabeth F. Martinez ◽  
Tatiani A.G. Donato ◽  
Victor E. Arana-Chavez
Keyword(s):  
Ascorbic Acid ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
In Vitro Effects

scholarly journals Evaluation of initial attachment of human gingival fibroblast cells to biodegradable membranes in vitro by light and scanning electron microscopy.

1999 ◽  
Vol 41 (2) ◽  
pp. 57-60 ◽  
Author(s):  
Berrin Ünsal ◽  
Gönen Özcan ◽  
Gülay Tüter ◽  
Bülent Kurtis ◽  
Mehmet Yalim
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Initial Attachment ◽  
Scanning Electron

scholarly journals Anti-inflammatory effects of zinc in PMA-treated human gingival fibroblast cells

2015 ◽  
pp. e180-e187 ◽  
Author(s):  
J. Kim ◽  
S. Kim ◽  
S. Jeon ◽  
Z. Hui ◽  
Y. Kim ◽  
...  
Keyword(s):  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Cells ◽  
Anti Inflammatory

scholarly journals Enhanced Human Gingival Fibroblast Response and Reduced Porphyromonas gingivalis Adhesion with Titania Nanotubes

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Zhiqiang Xu ◽  
Yuqi He ◽  
Xiufeng Zeng ◽  
Xiuxia Zeng ◽  
Junhui Huang ◽  
...  
Keyword(s):  
Dental Implants ◽  
Porphyromonas Gingivalis ◽  
Soft Tissues ◽  
Titania Nanotubes ◽  
Biological Behavior ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Related Gene ◽  
Gene Expressions ◽  
Tissue Integration

Successful dental implants rely on stable osseointegration and soft-tissue integration. Titania nanotubes (TNTs) with a diameter of 100 nm could increase the mesenchymal stem cell response and simultaneously decrease Staphylococcus aureus adhesion. However, the interactions between the modified surface and surrounding soft tissues are still unknown. In the present study, we fully investigated the biological behavior of human gingival fibroblasts (HGFs) and the adhesion of Porphyromonas gingivalis (P. gingivalis). TNTs were synthesized on titanium (Ti) surfaces by electrochemical anodization at 10, 30, and 60 V, and the products were denoted as NT10, NT30, and NT60, respectively. NT10 (diameter: 30 nm) and NT30 (diameter: 100 nm) could enhance the HGF functions, such as cell attachment and proliferation and extracellular matrix- (ECM-) related gene expressions, with the latter showing higher enhancement. NT60 (diameter: 200 nm) clearly impaired cell adhesion and proliferation and ECM-related gene expressions. Bacterial adhesion on the TNTs decreased and reached the lowest value on NT30. Therefore, NT30 without pharmaceuticals can be used to substantially enhance the HGF response and reduce P. gingivalis adhesion to the utmost, thus demonstrating significant potential in the transgingival part of dental implants.

In vitro anti-tumor effect of low-power laser irradiation (LPLI) on gastroenterological carcinoma cells

2019 ◽  
Vol 35 (3) ◽  
pp. 677-685 ◽  
Author(s):  
Ye Tian ◽  
Yeachan Lee ◽  
Hyejin Kim ◽  
Hyun Wook Kang
Keyword(s):  
Low Power ◽  
Laser Irradiation ◽  
Carcinoma Cells ◽  
Power Laser
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