Cerebral, cardiac and skeletal muscle stress associated with a series of static and dynamic apnoeas

P.136DNAJB6 integrates sarcomere protein homeostasis and myogenic signaling in coordinated skeletal muscle stress response

Neuromuscular Disorders ◽

10.1016/j.nmd.2019.06.192 ◽

2019 ◽

Vol 29 ◽

pp. S87-S88

Author(s):

A. Findlay ◽

R. Bengoechea ◽

S. Pittman ◽

C. Weihl

Keyword(s):

Skeletal Muscle ◽

Stress Response ◽

Protein Homeostasis ◽

Muscle Stress

Simvastatin Alters the RhoA Adaptation to Skeletal Muscle Stress Conditions

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000487850.18826.f5 ◽

2016 ◽

Vol 48 ◽

pp. 951

Author(s):

David R. Amici ◽

Dapeng Chen ◽

Eva R. Chin

Keyword(s):

Skeletal Muscle ◽

Stress Conditions ◽

Muscle Stress

The effect of exercise-intensity on skeletal muscle stress kinase and insulin protein signaling

PLoS ONE ◽

10.1371/journal.pone.0171613 ◽

2017 ◽

Vol 12 (2) ◽

pp. e0171613 ◽

Cited By ~ 12

Author(s):

Lewan Parker ◽

Adam Trewin ◽

Itamar Levinger ◽

Christopher S. Shaw ◽

Nigel K. Stepto

Keyword(s):

Skeletal Muscle ◽

Exercise Intensity ◽

Protein Signaling ◽

Stress Kinase ◽

Muscle Stress

Skeletal Muscle Stress Protein mRNA Response to Aerobic Exercise in Different Environmental Temperatures

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000562012.24169.05 ◽

2019 ◽

Vol 51 (Supplement) ◽

pp. 503

Author(s):

Megan J. Johnson ◽

Rebecca L. Cuthbert ◽

Robert J. Shute ◽

Dustin R. Slivka

Keyword(s):

Skeletal Muscle ◽

Aerobic Exercise ◽

Stress Protein ◽

Muscle Stress ◽

Environmental Temperatures

The influence of physical dimension on apparent stress–strain behaviour of in vitro passive skeletal muscle samples

The Journal of Strain Analysis for Engineering Design ◽

10.1177/0309324716668673 ◽

2016 ◽

Vol 52 (1) ◽

pp. 3-11 ◽

Cited By ~ 1

Author(s):

Ciaran Simms ◽

Hannah Kilroy ◽

Gary Blackburn ◽

Michael Takaza

Keyword(s):

Skeletal Muscle ◽

Stress Responses ◽

Stretch Ratio ◽

Dynamic Tests ◽

Stress Strain ◽

Porcine Tissue ◽

Sample Dimension ◽

Strain Behaviour ◽

Muscle Stress

The stress–strain behaviour of skeletal muscle is affected by many factors, leading to varied results reported in the literature. This article examines how the physical dimension of samples in in vitro compression tests affects the muscle stress for a given stretch ratio, in both quasi-static and dynamic loading. It is proposed that physically larger samples display a higher stress response due to the greater inclusion of complete muscle fascicles and also a reduction in percentage fluid exudation during compression. In the case of quasi-static loading, this was evaluated by testing nominally cubic samples of fresh and aged porcine tissue of characteristic lengths between 10 and 40 mm in compression at 0.05%/s strain in the fibre and cross-fibre directions using a Zwick Z005 universal testing rig. For the dynamic tests, a custom instrumented drop tower test rig was used to achieve average strain rates of 12,500%/s, and the stress responses at stretch ratios of [Formula: see text] 0.8 and [Formula: see text] 0.5 of nominally cubic samples of aged porcine tissue of characteristic lengths between 10 and 30 mm compressively loaded in the cross-fibre direction were evaluated. Both static and dynamic results clearly indicate that the muscle stress for a given stretch ratio of aged skeletal muscle tissue increases as the characteristic sample dimension increases from 10 to 30 mm. The effect was somewhat stronger for the dynamic tests. In the case of quasi-static testing, the strain rate of 0.05%/s limited the influence of the viscoelastic properties of the muscle, and sample dimension effects in the static tests are mostly attributable to the greater proportion of complete fascicles in the physically larger samples. In dynamic testing, in addition to the proportion of complete fascicle inclusion, the smaller percentage of fluid exudation for the larger samples compared to the smaller samples may also influence the size effect.

Double-blind study of metaxalone; use as skeletal-muscle relaxant

JAMA ◽

10.1001/jama.195.6.479 ◽

1966 ◽

Vol 195 (6) ◽

pp. 479-480 ◽

Cited By ~ 3

Author(s):

S. Diamond

Keyword(s):

Skeletal Muscle ◽

Muscle Relaxant ◽

Blind Study ◽

Double Blind Study ◽

Double Blind ◽

Skeletal Muscle Relaxant

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100050755 ◽

1974 ◽

Vol 32 ◽

pp. 148-149

Author(s):

D. E. Philpott ◽

A. Takahashi

Keyword(s):

Skeletal Muscle ◽

Endothelial Cells ◽

Salivary Gland ◽

Carotid Artery ◽

Basement Membrane ◽

Coronary Vessels ◽

Ruthenium Red ◽

E Coli ◽

Old Rats ◽

The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Ultra-Rapid Freezing of Isolated Skeletal Muscle Fibers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080316 ◽

1977 ◽

Vol 35 ◽

pp. 576-577

Author(s):

Joachim R. Sommer ◽

Nancy R. Wallace

Keyword(s):

Skeletal Muscle ◽

Sarcoplasmic Reticulum ◽

Granular Material ◽

Nuclear Envelope ◽

Intracellular Calcium ◽

Ruthenium Red ◽

Threshold Concentration ◽

Rapid Freezing ◽

Frog Skeletal Muscle ◽

Electrical Isolation

After Howell (1) had shown that ruthenium red treatment of fixed frog skeletal muscle caused collapse of the intermediate cisternae of the sarcoplasmic reticulum (SR), forming a pentalaminate structure by obi iterating the SR lumen, we demonstrated that the phenomenon involves the entire SR including the nuclear envelope and that it also occurs after treatment with other cations, including calcium (2,3,4).From these observations we have formulated a hypothesis which states that intracellular calcium taken up by the SR at the end of contraction causes the M rete to collapse at a certain threshold concentration as the first step in a subsequent centrifugal zippering of the free SR toward the junctional SR (JSR). This would cause a) bulk transport of SR contents, such as calcium and granular material (4) into the JSR and, b) electrical isolation of the free SR from the JSR.

Electron Probe Analysis of Muscle

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054583 ◽

1982 ◽

Vol 40 ◽

pp. 398-401

Author(s):

A. V. Somlyo ◽

H. Shuman ◽

A. P. Somlyo

Keyword(s):

Skeletal Muscle ◽

Smooth Muscle ◽

Sarcoplasmic Reticulum ◽

Resting State ◽

Binding Sites ◽

Electron Probe ◽

Frog Skeletal Muscle ◽

Electron Probe Analysis ◽

Probe Analysis

Electron probe analysis of frozen dried cryosections of frog skeletal muscle, rabbit vascular smooth muscle and of isolated, hyperpermeab1 e rabbit cardiac myocytes has been used to determine the composition of the cytoplasm and organelles in the resting state as well as during contraction. The concentration of elements within the organelles reflects the permeabilities of the organelle membranes to the cytoplasmic ions as well as binding sites. The measurements of [Ca] in the sarcoplasmic reticulum (SR) and mitochondria at rest and during contraction, have direct bearing on their role as release and/or storage sites for Ca in situ.

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124288 ◽

1992 ◽

Vol 50 (1) ◽

pp. 776-777

Author(s):

Joachim R. Sommer ◽

Teresa High ◽

Betty Scherer ◽

Isaiah Taylor ◽

Rashid Nassar

Keyword(s):

Skeletal Muscle ◽

High Resolution ◽

Action Potential ◽

Muscle Fiber ◽

Freeze Fracture ◽

Freeze Substitution ◽

Electrical Field Stimulation ◽

Skeletal Muscle Fiber ◽

Freeze Dried ◽

Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.