A tomato NAC transcription factor, SlNAM1, positively regulates ethylene biosynthesis and the onset of tomato fruit ripening

2021 ◽  
Author(s):  
Ying Gao ◽  
Zhong‐Qi Fan ◽  
Qiang Zhang ◽  
Hong‐Li Li ◽  
Gang‐Shuai Liu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Ethylene Biosynthesis ◽  
Nac Transcription Factor ◽  
Tomato Fruit Ripening

scholarly journals A NAC transcription factor, NOR-like1, is a new positive regulator of tomato fruit ripening

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Xiaodan Zhao ◽  
Xiaoli Tan ◽  
Zhongqi Fan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Nac Transcription Factor ◽  
Positive Regulator ◽  
Tomato Fruit Ripening

scholarly journals Corrigendum to: Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

2020 ◽  
Vol 71 (12) ◽  
pp. 3759-3759
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Zhongqi Fan ◽  
Xiaodan Zhao ◽  
Yiping Zhang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Tomato Fruit Ripening

Unraveling the target genes of RIN transcription factor during tomato fruit ripening and softening

2016 ◽  
Vol 97 (3) ◽  
pp. 991-1000 ◽  
Author(s):  
Ling Li ◽  
Xiaoguang Wang ◽  
Xinhua Zhang ◽  
Mei Guo ◽  
Tieling Liu
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Target Genes ◽  
Tomato Fruit ◽  
Tomato Fruit Ripening

scholarly journals Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

2020 ◽  
Vol 71 (12) ◽  
pp. 3560-3574 ◽  
Author(s):  
Ying Gao ◽  
Wei Wei ◽  
Zhongqi Fan ◽  
Xiaodan Zhao ◽  
Yiping Zhang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Pectate Lyase ◽  
Ethylene Biosynthesis ◽  
Fruit Softening ◽  
Wild Type ◽  
Gain Of Function ◽  
Carotenoid Accumulation ◽  
Acid Protein ◽  
Tomato Fruit Ripening

Abstract The tomato non-ripening (nor) mutant generates a truncated 186-amino-acid protein (NOR186) and has been demonstrated previously to be a gain-of-function mutant. Here, we provide more evidence to support this view and answer the open question of whether the NAC-NOR gene is important in fruit ripening. Overexpression of NAC-NOR in the nor mutant did not restore the full ripening phenotype. Further analysis showed that the truncated NOR186 protein is located in the nucleus and binds to but does not activate the promoters of 1-aminocyclopropane-1-carboxylic acid synthase2 (SlACS2), geranylgeranyl diphosphate synthase2 (SlGgpps2), and pectate lyase (SlPL), which are involved in ethylene biosynthesis, carotenoid accumulation, and fruit softening, respectively. The activation of the promoters by the wild-type NOR protein can be inhibited by the mutant NOR186 protein. On the other hand, ethylene synthesis, carotenoid accumulation, and fruit softening were significantly inhibited in CR-NOR (CRISPR/Cas9-edited NAC-NOR) fruit compared with the wild-type, but much less severely affected than in the nor mutant, while they were accelerated in OE-NOR (overexpressed NAC-NOR) fruit. These data further indicated that nor is a gain-of-function mutation and NAC-NOR plays a significant role in ripening of wild-type fruit.

The basic helix-loop-helix transcription factor bHLH95 affects fruit ripening and multiple metabolisms in tomato

2020 ◽  
Vol 71 (20) ◽  
pp. 6311-6327
Author(s):  
Lincheng Zhang ◽  
Jing Kang ◽  
Qiaoli Xie ◽  
Jun Gong ◽  
Hui Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fruit Ripening ◽  
Tomato Fruit ◽  
Soluble Sugar ◽  
Glutathione Metabolism ◽  
Bhlh Transcription Factor ◽  
Total Carotenoids ◽  
Helix Loop Helix ◽  
Tomato Fruit Ripening

Abstract Ethylene signaling pathways regulate several physiological alterations that occur during tomato fruit ripening, such as changes in colour and flavour. The mechanisms underlying the transcriptional regulation of genes in these pathways remain unclear, although the role of the MADS-box transcription factor RIN has been widely reported. Here, we describe a bHLH transcription factor, SlbHLH95, whose transcripts accumulated abundantly in breaker+4 and breaker+7 fruits compared with rin (ripening inhibitor) and Nr (never ripe) mutants. Moreover, the promoter activity of SlbHLH95 was regulated by RIN in vivo. Suppression of SlbHLH95 resulted in reduced sensitivity to ethylene, decreased accumulation of total carotenoids, and lowered glutathione content, and inhibited the expression of fruit ripening- and glutathione metabolism-related genes. Conversely, up-regulation of SlbHLH95 in wild-type tomato resulted in higher sensitivity to ethylene, increased accumulation of total carotenoids, slightly premature ripening, and elevated accumulation of glutathione, soluble sugar, and starch. Notably, overexpression of SlbHLH95 in rin led to the up-regulated expression of fruit ripening-related genes (FUL1, FUL2, SAUR69, ERF4, and CNR) and multiple glutathione metabolism-related genes (GSH1, GSH2, GSTF1, and GSTF5). These results clarified that SlbHLH95 participates in the regulation of fruit ripening and affects ethylene sensitivity and multiple metabolisms targeted by RIN in tomato.

scholarly journals Comprehensive Analysis of ABA Effects on Ethylene Biosynthesis and Signaling during Tomato Fruit Ripening

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0154072 ◽  
Author(s):  
Wangshu Mou ◽  
Dongdong Li ◽  
Jianwen Bu ◽  
Yuanyuan Jiang ◽  
Zia Ullah Khan ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Ethylene Biosynthesis ◽  
Comprehensive Analysis ◽  
Tomato Fruit Ripening ◽  
Ethylene Biosynthesis And Signaling

scholarly journals Sound waves delay tomato fruit ripening by negatively regulating ethylene biosynthesis and signaling genes

2015 ◽  
Vol 110 ◽  
pp. 43-50 ◽  
Author(s):  
Joo-Yeol Kim ◽  
Jin-Su Lee ◽  
Taek-Ryoun Kwon ◽  
Soo-In Lee ◽  
Jin-A. Kim ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Tomato Fruit ◽  
Ethylene Biosynthesis ◽  
Sound Waves ◽  
Tomato Fruit Ripening ◽  
Ethylene Biosynthesis And Signaling
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