Corrigendum to: Re-evaluation of the nor mutation and the role of the NAC-NOR transcription factor in tomato fruit ripening

Abstract Ethylene signaling pathways regulate several physiological alterations that occur during tomato fruit ripening, such as changes in colour and flavour. The mechanisms underlying the transcriptional regulation of genes in these pathways remain unclear, although the role of the MADS-box transcription factor RIN has been widely reported. Here, we describe a bHLH transcription factor, SlbHLH95, whose transcripts accumulated abundantly in breaker+4 and breaker+7 fruits compared with rin (ripening inhibitor) and Nr (never ripe) mutants. Moreover, the promoter activity of SlbHLH95 was regulated by RIN in vivo. Suppression of SlbHLH95 resulted in reduced sensitivity to ethylene, decreased accumulation of total carotenoids, and lowered glutathione content, and inhibited the expression of fruit ripening- and glutathione metabolism-related genes. Conversely, up-regulation of SlbHLH95 in wild-type tomato resulted in higher sensitivity to ethylene, increased accumulation of total carotenoids, slightly premature ripening, and elevated accumulation of glutathione, soluble sugar, and starch. Notably, overexpression of SlbHLH95 in rin led to the up-regulated expression of fruit ripening-related genes (FUL1, FUL2, SAUR69, ERF4, and CNR) and multiple glutathione metabolism-related genes (GSH1, GSH2, GSTF1, and GSTF5). These results clarified that SlbHLH95 participates in the regulation of fruit ripening and affects ethylene sensitivity and multiple metabolisms targeted by RIN in tomato.

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Regulatory Role of Cystathionine-γ-Synthase and de novo Synthesis of Methionine in Ethylene Production during tomato Fruit Ripening

Plant Molecular Biology ◽

10.1007/s11103-006-0009-8 ◽

2006 ◽

Vol 61 (1-2) ◽

pp. 255-268 ◽

Cited By ~ 34

Author(s):

Yael S. Katz ◽

Gad Galili ◽

Rachel Amir

Keyword(s):

Fruit Ripening ◽

Ethylene Production ◽

De Novo ◽

Tomato Fruit ◽

Regulatory Role ◽

De Novo Synthesis ◽

Tomato Fruit Ripening

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A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening

Nature Genetics ◽

10.1038/ng1841 ◽

2006 ◽

Vol 38 (8) ◽

pp. 948-952 ◽

Cited By ~ 673

Author(s):

Kenneth Manning ◽

Mahmut Tör ◽

Mervin Poole ◽

Yiguo Hong ◽

Andrew J Thompson ◽

...

Keyword(s):

Transcription Factor ◽

Fruit Ripening ◽

Tomato Fruit ◽

Gene Encoding ◽

Naturally Occurring ◽

Tomato Fruit Ripening

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Regulation of Postharvest Tomato Fruit Ripening by Endogenous Salicylic Acid

Frontiers in Plant Science ◽

10.3389/fpls.2021.663943 ◽

2021 ◽

Vol 12 ◽

Author(s):

Chunoti Changwal ◽

Tushita Shukla ◽

Zakir Hussain ◽

Neera Singh ◽

Abhijit Kar ◽

...

Keyword(s):

Lipid Peroxidation ◽

Salicylic Acid ◽

Carboxylic Acid ◽

Fruit Ripening ◽

Tomato Fruit ◽

Stability Index ◽

Acid Oxidase ◽

Tomato Fruit Ripening ◽

Lycopene Content

Exogenous application of salicylic acid (SA) has been known for delaying ripening in many fruit and vegetables. But the function of endogenous SA in relation to postharvest fruit performance is still unexplored. To understand the role of endogenous SA in postharvest fruit ripening of tomato, 33 tomato lines were examined for their endogenous SA content, membrane stability index (MSI), and shelf life (SL) at turning and red stages of tomato fruit ripening. Six tomato lines having contrasting shelf lives from these categories were subjected further for ethylene (ET) evolution, 1-aminocyclopropane-1-carboxylic acid synthase (ACS), 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), polygalacturonase (PG), pectin methyl esterase (PME), antioxidant assays and lipid peroxidation. It was found that high endogenous SA has a direct association with low ET evolution, which leads to the high SL of fruit. High lycopene content was also found to be correlated with high SA. Total antioxidants, PG, and PME decreased and lipid peroxidation increased from turning to red stage of tomato fruit development. Furthermore, these lines were subjected to expression analysis for SA biosynthesis enzymes viz. Solanum lycopersicum Isochorismate Synthase (SlICS) and SlPAL. Real-time PCR data revealed that high SL lines have high SlPAL4 expression and low SL lines have high SlPAL6 expression. Based on the results obtained in this study, it was concluded that endogenous SA regulates ET evolution and SL with the aid of the antioxidative defense system, and SlPAL4 and SlPAL6 genes play significant but opposite roles during fruit ripening.

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Molecular and functional characterization of the SBP-box transcription factor SPL-CNR in tomato fruit ripening and cell death

Journal of Experimental Botany ◽

10.1093/jxb/eraa067 ◽

2020 ◽

Vol 71 (10) ◽

pp. 2995-3011 ◽

Cited By ~ 1

Author(s):

Tongfei Lai ◽

Xiaohong Wang ◽

Bishun Ye ◽

Mingfei Jin ◽

Weiwei Chen ◽

...

Keyword(s):

Cell Death ◽

Transcription Factor ◽

Nuclear Localization ◽

Fruit Ripening ◽

Tomato Fruit ◽

Functional Characterization ◽

Binding Activity ◽

Multifunctional Protein ◽

Tomato Fruit Ripening ◽

Tf Gene

Abstract SlSPL-CNR, an SBP-box transcription factor (TF) gene residing at the epimutant Colourless non-ripening (Cnr) locus, is involved in tomato ripening. This epimutant provides a unique model to investigate the (epi)genetic basis of fruit ripening. Here we report that SlSPL-CNR is a nucleus-localized protein with a distinct monopartite nuclear localization signal (NLS). It consists of four consecutive residues ‘ 30KRKR33’ at the N-terminus of the protein. Mutation of the NLS abolishes SlSPL-CNR’s ability to localize in the nucleus. SlSPL-CNR comprises two zinc-finger motifs (ZFMs) within the C-terminal SBP-box domain. Both ZFMs contribute to zinc-binding activity. SlSPL-CNR can induce cell death in tomato and tobacco, dependent on its nuclear localization. However, the two ZFMs have differential impacts on SlSPL-CNR’s induction of severe necrosis or mild necrotic ringspot. NLS and ZFM mutants cannot complement Cnr fruits to ripen. SlSPL-CNR interacts with SlSnRK1. Virus-induced SlSnRK1 silencing leads to reduction in expression of ripening-related genes and inhibits ripening in tomato. We conclude that SlSPL-CNR is a multifunctional protein that consists of a distinct monopartite NLS, binds to zinc, and interacts with SlSnRK1 to affect cell death and tomato fruit ripening.

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