Evaluation of hematologic, biochemical, and blood gas variables in stored canine packed red blood cells, and the impact of storage time on blood recipients

2020 ◽  
Vol 49 (2) ◽  
pp. 198-206
Author(s):  
Renata R. Rodrigues ◽  
Caroline Y. Kayano ◽  
Vinícius P. Santos ◽  
Ludmila R. Moroz ◽  
Denise T. Fantoni ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Storage Time ◽  
Blood Gas ◽  
Packed Red Blood Cells ◽  
The Impact

Erythroplateletapheresis: A New Strategy in the Global Apheresis Program

1998 ◽  
Vol 21 (6_suppl) ◽  
pp. 17-19
Author(s):  
G. Menichella ◽  
M. Ciarli ◽  
R. Serafini ◽  
L. Pierelli ◽  
M. Vittori ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Plasma Volume ◽  
Blood Cells ◽  
Storage Time ◽  
Blood Donation ◽  
Mean Values ◽  
Packed Red Blood Cells ◽  
New Strategy ◽  
The Mean

Blood donation allowed by cell separators can offer higher performance and higher yield to guarantee better quality and pureness of collected products. New systems for the collection of platelet concentrate (PC) and packed red blood cells (PRBC) are currently available. The aim of our work was to test the possibility of preparing PC routinely from normal apheresis donors in a minimum amount of time while providing a second product. Over a 3-month period we performed 40 procedures using the Hemonetics MCS3P blood cell separator and the Dideco Excel. The mean values of platelet yield were 2.8 x 1011 (range 1.4-4.1) with the MCS3P and 3.49 x 1011 (range 2.9-3.9) with the Excel, in a plasma volume of 240 ml and 215 ml respectively; the PRBC units were added with SAG-Mannitol allowing a storage time of 42 days. Collection times were 71’ and 48’ respectively. Donor tolerance was analogous to phateletapheresis or plasmapheresis.

When Enough is Enough: Impact of Packed Red Blood Cells in Massive Transfusion Outcomes

2013 ◽  
Vol 79 (8) ◽  
pp. 810-814 ◽  
Author(s):  
Kira Long ◽  
Jiselle Bock Heaney ◽  
Eric R. Simms ◽  
Norman E. McSwain ◽  
Juan C. Duchesne
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Survival Benefit ◽  
Massive Transfusion ◽  
Fresh Frozen Plasma ◽  
Survival Advantage ◽  
Packed Red Blood Cells ◽  
Fresh Frozen ◽  
Level I ◽  
The Impact

Massive transfusion protocol (MTP) with fresh-frozen plasma and packed red blood cells (PRBCs) in a 1:1 ratio is one of the most common resuscitative strategies used in patients with severe hemorrhage. There are no studies to date that examine the best postoperative hematocrit range as a marker for survival after MTP. We hypothesize a postoperative hematocrit dose-dependent survival benefit in patients receiving MTP. This was a 53-month retrospective analysis of patients with intra-abdominal injuries requiring surgery and transfusion of 10 units PRBCs or more at a single Level I trauma center. Groups were defined by postoperative hematocrit (less than 21, 21 to 29, 29.1 to 39, and 39 or more). Kaplan-Meier (KM) survival probability was calculated. One hundred fifty patients requiring operative abdominal explorations and 10 units PRBCs or more were identified. There were no significant differences in demographics between groups. When comparing postoperative hematocrit groups, relative to a hematocrit of less than 21 per cent in KM survival analysis, an overall survival advantage was only evident in patients transfused to hematocrits 29.1 to 39 per cent ( P < 0.03; odds ratio [OR], 0.284; 95% confidence interval [CI], 0.089 to 0.914). This survival advantage was not seen in the other groups (21 to 29: OR, 0.352; 95% CI, 0.103 to 1.195 or 39% or greater: OR, 0.107; 95% CI, 0.010 to 1.121). This is the first study to examine the impact of postoperative hematocrit as an indicator of survival after MTP in the trauma patient. Transfusion to hematocrits between 29.1 and 39 per cent conveyed a survival benefit, whereas resuscitation to supraphysiologic hematocrits 39 per cent or greater conveyed no additional survival benefit. This study highlights the need for judicious PRBC administration during MTP and its potential impact on survival in patients with postoperative supraphysiologic hematocrits.

scholarly journals RELATIONSHIP BETWEEN TRANSFUSION OF PACKED RED BLOOD CELLS, PLASMA FREE HEMOGLOBIN AND STORAGE TIME IN PATIENTS WITH SEVERE TRAUMA

2015 ◽  
Vol 3 (Suppl 1) ◽  
pp. A916
Author(s):  
S Chacón Alves ◽  
M Chico Fernández ◽  
C García Fuentes ◽  
A Del Pino Ramírez ◽  
N Zurera Plaza ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Storage Time ◽  
Severe Trauma ◽  
Free Hemoglobin ◽  
Packed Red Blood Cells ◽  
And Storage

scholarly journals An Insight into the Stages of Ion Leakage during Red Blood Cell Storage

2021 ◽  
Vol 22 (6) ◽  
pp. 2885
Author(s):  
Anna Zimna ◽  
Magdalena Kaczmarska ◽  
Ewa Szczesny-Malysiak ◽  
Aleksandra Wajda ◽  
Katarzyna Bulat ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Properties ◽  
Ion Leakage ◽  
Packed Red Blood Cells ◽  
Force Microscopy ◽  
Group Type ◽  
Singly Charged ◽  
The Impact

Packed red blood cells (pRBCs), the most commonly transfused blood product, are exposed to environmental disruptions during storage in blood banks. In this study, temporal sequence of changes in the ion exchange in pRBCs was analyzed. Standard techniques commonly used in electrolyte measurements were implemented. The relationship between ion exchange and red blood cells (RBCs) morphology was assessed with use of atomic force microscopy with reference to morphological parameters. Variations observed in the Na+, K+, Cl−, H+, HCO3−, and lactate ions concentration show a complete picture of singly-charged ion changes in pRBCs during storage. Correlation between the rate of ion changes and blood group type, regarding the limitations of our research, suggested, that group 0 is the most sensitive to the time-dependent ionic changes. Additionally, the impact of irreversible changes in ion exchange on the RBCs membrane was observed in nanoscale. Results demonstrate that the level of ion leakage that leads to destructive alterations in biochemical and morphological properties of pRBCs depend on the storage timepoint.

scholarly journals Does the Age of Packed Red Blood Cells, Donor Sex or Sex Mismatch Affect the Sublingual Microcirculation in Critically Ill Intensive Care Unit Patients? — A Secondary Interpretation of a Retrospective Analysis

2021 ◽  
Author(s):  
Demian Knobel ◽  
Jonas Scheuzger ◽  
Andreas Buser ◽  
Alexa Hollinger ◽  
Caroline E. Gebhard ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Red Blood Cells ◽  
Sofa Score ◽  
Blood Cells ◽  
Flow Index ◽  
Packed Red Blood Cells ◽  
Microcirculatory Flow ◽  
The Impact

Abstract BackgroundIn vitro studies have thoroughly documented the impact of storage lesions in packed red blood cells (pRBC) on erythrocyte oxygen carrying capacity due to older age of blood products. While studies have examined the effect of pRBC age on patient outcome, only few data exist on the microcirculation, their primary site of action.MethodsIn this secondary analysis, we examined the relationship between the age of pRBC and changes of microcirculatory flow (MCF) in 54 patients. Data from the Basel Bedside assessment Microcirculation Transfusion Limit study (Ba2MiTraL), investigating the effects of one pRBC on the sublingual MCF provided the basis of this study. ResultsMean change from pre-to post-transfusion proportion of perfused vessels (∆PPV) was +8.8% (IQR: -0.5 – 22.5), 5.5% (IQR: 0.1–10.1), and +4.7% (IQR: -2.1 – 6.5) after transfusion of fresh (≤ 14 days old), medium (15 to 34 days old), and old (≥ 35 days old) pRBC, respectively. Values for the microcirculatory flow index (MFI) were +0.22 (IQR: -0.1 – 0.6), +0.22 (IQR: 0.0 – 0.3), and +0.06 (IQR: -0.1 – 0.3) for the fresh, medium, and old pRBC age groups, respectively.Lower ∆PPV and transfusion of older blood were correlated with a higher Sequential Organ Failure Assessment (SOFA) score of patients upon admission to the intensive care unit (ICU) (p=0.01). However, regression models showed no overall significant correlation between pRBC age and ∆PPV (p=0.2). No correlation between donor’s sex or a mismatch between donor and recipient sex was found.ConclusionWe detected no significant correlation between age of pRBC and change in MCF between pre- and post-transfusion among all investigated patients. However, in patients with a higher SOFA score upon ICU admission, there might be a negative effect on the proportion of perfused microcirculatory vessels after transfusion of older blood.

scholarly journals Time Dependent Assessment of Morphological Changes: Leukodepleted Packed Red Blood Cells Stored in SAGM

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Ibrahim Mustafa ◽  
Asma Al Marwani ◽  
Khuloud Mamdouh Nasr ◽  
Noora Abdulla Kano ◽  
Tameem Hadwan
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Storage Time ◽  
Morphological Changes ◽  
Storage Period ◽  
Osmotic Fragility ◽  
Prolonged Storage ◽  
Morphological Alterations ◽  
Packed Red Blood Cells ◽  
Stored Blood

Usually packed red blood cells (pRBCs) require specific conditions in storage procedures to ensure the maximum shelf life of up to 42 days in 2–6°C. However, molecular and biochemical consequences can affect the stored blood cells; these changes are collectively labeled as storage lesions. In this study, the effect of prolonged storage was assessed through investigating morphological changes and evaluating oxidative stress. Samples from leukodepleted pRBC in SAGM stored at 4°C for 42 days were withdrawn aseptically on day 0, day 14, day 28, and day 42. Morphological changes were observed using scanning electron microscopy and correlated with osmotic fragility and hematocrit. Oxidative injury was studied through assessing MDA level as a marker for lipid peroxidation. Osmotic fragility test showed that extended storage time caused increase in the osmotic fragility. The hematocrit increased by 6.6% from day 0 to day 42. The last 2 weeks show alteration in the morphology with the appearance of echinocytes and spherocytes. Storage lesions and morphological alterations appeared to affect RBCs during the storage period. Further studies should be performed to develop strategies that will aid in the improvement of stored pRBC quality and efficacy.

scholarly journals Transfusion of Packed Red Blood Cells is Not Associated with Improved Central Venous Oxygen Saturation or Organ Function in Patients with Septic Shock

2012 ◽  
Vol 43 (4) ◽  
pp. 593-598 ◽  
Author(s):  
Brian M. Fuller ◽  
Mithil Gajera ◽  
Christa Schorr ◽  
David Gerber ◽  
R. Phillip Dellinger ◽  
...  
Keyword(s):  
Septic Shock ◽  
Oxygen Saturation ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Central Venous Oxygen Saturation ◽  
Organ Function ◽  
Central Venous ◽  
Packed Red Blood Cells ◽  
Venous Oxygen Saturation

scholarly journals Blood Donations and Transfusions during the COVID-19 Pandemic in Spain: Impact According to Autonomous Communities and Hospitals

2021 ◽  
Vol 18 (7) ◽  
pp. 3480
Author(s):  
José Antonio García-Erce ◽  
Íñigo Romón-Alonso ◽  
Carlos Jericó ◽  
José María Domingo-Morera ◽  
José Luis Arroyo-Rodríguez ◽  
...  
Keyword(s):  
Blood Cells ◽  
Blood Components ◽  
Health Ministry ◽  
State Of Emergency ◽  
Blood Donations ◽  
Autonomous Communities ◽  
Packed Red Blood Cells ◽  
Average Decrease ◽  
A Prospective Study ◽  
The Impact

Worldwide, the COVID-19 pandemic has caused a decline in blood donations, between 30% and 70% in some of the most affected countries. In Spain, during the initial eight weeks after the State of Emergency was decreed on 14 March 2020, in the weekly reports of the Health Ministry, an average decrease of 20% was observed between 11 and week 25 compared with the 2018 donation. We aimed to investigate the impact of the COVID-19 pandemic on blood donations and blood distribution in four autonomous communities, and to explore the evolution of the consumption of blood components (BCs) in ten hospitals of six autonomous communities. We performed a prospective study of grouped cohorts on the donation and distribution of blood in four regional transfusion centers in four autonomous communities in Spain, and a retrospective study of the consumption of blood components in ten hospitals in six autonomous communities. Regarding donations, there was no significant decrease in donations, with differences between autonomous communities, which started between 1 and 15 March 2020 (−11%). The increase in donations in phase II (from 26 May 2020) stands out. Regarding consumption, there was a significant reduction in the consumption of packed red blood cells (RBCs) (24.5%), plasma (45.3%), and platelets (25.3%) in the central period (16 March–10 May). The reduction in the consumption of RBCs was significant in the period from 1–15 March. Conclusions: The COVID-19 pandemic has affected the donation and consumption of BCs.

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