Candida parapsilosis complex infection in a cutaneous lesion from a dog: Spotlight on an opportunistic pathogen of potential increasing importance

2020 ◽  
Author(s):  
Niki Skeldon ◽  
Melanie Dobromylskyj ◽  
Debra Fews ◽  
William Hayden
Keyword(s):  
Candida Parapsilosis ◽  
Cutaneous Lesion ◽  
Opportunistic Pathogen ◽  
Candida Parapsilosis Complex

Exposure of Candida parapsilosis complex to agricultural azoles: An overview of the role of environmental determinants for the development of resistance

2019 ◽  
Vol 650 ◽  
pp. 1231-1238 ◽  
Author(s):  
Raimunda Sâmia Nogueira Brilhante ◽  
Lucas Pereira de Alencar ◽  
Silviane Praciano Bandeira ◽  
Jamille Alencar Sales ◽  
Antônio José de Jesus Evangelista ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Environmental Determinants ◽  
Development Of Resistance ◽  
Candida Parapsilosis Complex

scholarly journals Rapid Detection of Echinocandins Resistance by MALDI-TOF MS in Candida parapsilosis Complex

2020 ◽  
Vol 8 (1) ◽  
pp. 109
Author(s):  
Ana Emília M. Roberto ◽  
Danilo E. Xavier ◽  
Esteban E. Vidal ◽  
Cláudia Fernanda de L. Vidal ◽  
Rejane P. Neves ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Incubation Time ◽  
Candida Parapsilosis ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Reference Method ◽  
Sensu Stricto ◽  
Maldi Tof ◽  
Separation Parameter ◽  
Candida Parapsilosis Complex

Mass spectrometry by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) was used to identify and differentiate the pattern of susceptibility of clinical isolates of Candida parapsilosis complex. 17 C. parapsilosis sensu stricto, 2 C. orthopsilosis, and 1 C. metapsilosis strains were obtained from blood cultures, and three different inocula (103, 105, and 107 CFU/mL) were evaluated against three echinocandins at concentrations ranging from 0.03 to 16 µg/mL after incubation of 1 h, 2 h, and 3 h. Drug-free control was used. The spectra obtained at these concentrations were applied to generate composite correlation index (CCI) matrices for each yeast individually. After cross correlations and autocorrelations of each spectra with null (zero) and maximal (16) concentrations, the CCI was used as separation parameter among spectra. Incubation time and inoculum were critical factors to reach higher precision and reliability of this trial. With an incubation time of 3 h and inoculum of 107 CFU/mL, it was possible to determine the breakpoint of the clinical yeasts by MALDI-TOF that presented high agreement with the clinical laboratory standard institute (CLSI) reference method. Herein, we show that mass spectrometry using the MALDI-TOF technique is powerful when it exploits antifungal susceptibility testing assays.

scholarly journals Capability of Fluorescent Capillary Electrophoresis To Distinguish Species of the Candida parapsilosis Complex

2019 ◽  
Vol 57 (5) ◽  
Author(s):  
Iva Kotásková ◽  
Hana Obručova ◽  
Veronika Lýčková ◽  
Filip Růžička ◽  
Tomáš Freiberger
Keyword(s):  
Capillary Electrophoresis ◽  
Candida Parapsilosis ◽  
Candida Parapsilosis Complex

Qualitative and quantitative change of the tolerance to liposomal amphotericin B triggered by biofilm maturation in C. parapsilosis

2019 ◽  
Vol 58 (6) ◽  
pp. 827-834
Author(s):  
D Casagrande Pierantoni ◽  
L Roscini ◽  
L Corte ◽  
M Bernardo ◽  
M Bassetti ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Liposomal Amphotericin ◽  
Candida Parapsilosis ◽  
Opportunistic Pathogen ◽  
Hospital Environment ◽  
Liposomal Amphotericin B ◽  
Antibiofilm Activity ◽  
Planktonic Cells ◽  
Large Variability ◽  
Qualitative And Quantitative

Abstract Candida parapsilosis is an emerging opportunistic pathogen present in both clinical and natural environment, with a strong frequency of biofilm forming strains. While the drugs active against biofilm are rare, liposomal amphotericin B is credited with an antibiofilm activity in some opportunistic species of the genus Candida. Using freshly isolated strains from hospital environment, in this paper we could show the prevalence of biofilm forming vs. nonbiofilm forming strains. The former displayed a large variability in terms of biofilm biomass and metabolic activity. Liposomal amphotericin B minimum inhibitory concentration (MIC) of planktonic cells was below the breakpoint, whereas the sessile cells MIC (SMIC) was 1 or 2 orders of magnitude above the planktonic MIC. When the drug was applied to freshly attached cells, that is, biofilm in formation, the MIC (called SDMIC) was even below the MIC value. All resistance metrics (MIC, SMIC, and SDMIC) were quite variable although no correlation could be detected between them and the metrics used to quantify biofilm activity and biomass production. These findings demonstrate that young biofilm cells are even more susceptible than planktonic cells and that early treatments with this drug can be beneficial in cases of prosthesis implantation or especially when there is the necessity of a CVC reimplantation during a sepsis.

scholarly journals Species distribution, virulence factors, and antifungal susceptibility among Candida parapsilosis complex isolates from clinical specimens at Siriraj Hospital, Thailand, from 2011 to 2015

2017 ◽  
Vol 56 (4) ◽  
pp. 426-433 ◽  
Author(s):  
Sujiraphong Pharkjaksu ◽  
Piriyaporn Chongtrakool ◽  
Kamol Suwannakarn ◽  
Popchai Ngamskulrungroj
Keyword(s):  
Virulence Factors ◽  
Species Distribution ◽  
Candida Parapsilosis ◽  
Antifungal Susceptibility ◽  
Clinical Specimens ◽  
Siriraj Hospital ◽  
Candida Parapsilosis Complex

scholarly journals Identification and typing of the Candida parapsilosis complex: MALDI-TOF MS vs. AFLP

2014 ◽  
Vol 52 (2) ◽  
pp. 123-130 ◽  
Author(s):  
E. D. Carolis ◽  
L. A. M. Hensgens ◽  
A. Vella ◽  
B. Posteraro ◽  
M. Sanguinetti ◽  
...  
Keyword(s):  
Candida Parapsilosis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Candida Parapsilosis Complex ◽  
Tof Ms

scholarly journals Comparison of four molecular approaches to identify Candida parapsilosis complex species

2017 ◽  
Vol 112 (3) ◽  
pp. 214-219 ◽  
Author(s):  
Leonardo Silva Barbedo ◽  
Maria Helena Galdino Figueiredo-Carvalho ◽  
Mauro de Medeiros Muniz ◽  
Rosely Maria Zancopé-Oliveira
Keyword(s):  
Candida Parapsilosis ◽  
Complex Species ◽  
Molecular Approaches ◽  
Candida Parapsilosis Complex

scholarly journals Analysis of Inteins in the Candida parapsilosis Complex for Simple and Accurate Species Identification

2013 ◽  
Vol 51 (9) ◽  
pp. 2830-2836 ◽  
Author(s):  
T. H. R. Prandini ◽  
R. C. Theodoro ◽  
A. C. M. O. Bruder-Nascimento ◽  
C. M. Scheel ◽  
E. Bagagli
Keyword(s):  
Species Identification ◽  
Candida Parapsilosis ◽  
Candida Parapsilosis Complex

scholarly journals Corynebacterium striatum infecting a malignant cutaneous lesion: the emergence of an opportunistic pathogen

2009 ◽  
Vol 51 (2) ◽  
pp. 115-116 ◽  
Author(s):  
Silvana Vargas Superti ◽  
Daniela de Souza Martins ◽  
Juliana Caierão ◽  
Fabiana Soares ◽  
Taísa Prochnow ◽  
...  
Keyword(s):  
Soft Tissue ◽  
Dna Sequencing ◽  
Soft Tissue Infection ◽  
Cutaneous Lesion ◽  
Opportunistic Pathogen ◽  
Human Pathogen ◽  
Pathogen Identification ◽  
Neoplastic Lesion ◽  
Corynebacterium Striatum

We described a case of a 27-year old male patient with skin and soft tissue infection of a neoplastic lesion caused by Corynebacterium striatum, an organism which has been rarely described as a human pathogen. Identification was confirmed by DNA sequencing. Successful treatment with penicillin was achieved. The role of the C. striatum as an emerging opportunistic pathogen is discussed.

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