Genetic Variation Analysis of Korean Lotus ( Nelumbo nucifera ) using Randomly Amplified Polymorphic DNA ( RAPD ) Markers

1998 ◽  
Vol 28 (4) ◽  
pp. 343-355 ◽  
Author(s):  
Hyun Kim ◽  
Mi Jang Song ◽  
Ki Joomg Kim ◽  
Chong Woo Lee ◽  
Won Gil Chang ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Rapd Markers ◽  
Nelumbo Nucifera ◽  
Variation Analysis ◽  
Randomly Amplified Polymorphic Dna

Genetic diversity and relationships among Egyptian Galium (Rubiaceae) and related species using ISSR and RAPD markers

Biologia ◽  
2014 ◽  
Vol 69 (3) ◽  
Author(s):  
Kadry Abdel Khalik ◽  
Magdy Abd El-Twab ◽  
Rasha Galal
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Rapd Markers ◽  
Phylogenetic Analyses ◽  
Randomly Amplified Polymorphic Dna ◽  
Inter Simple Sequence Repeats ◽  
Close Relationship ◽  
Rapd And Issr ◽  
Simple Sequence ◽  
Issr Primers

AbstractGenetic diversity and phylogenetic analyses of 24 species, representing nine sections of the genus Galium (Rubiaceae), have been made using the Inter Simple Sequence Repeats (ISSR), Randomly Amplified Polymorphic DNA (RAPD), and combined ISSR and RAPD markers. Four ISSR primers and three RAPD primers generated 250 polymorphic amplified fragments. The results of this study showed that the level of genetic variation in Galium is relatively high. RAPD markers revealed a higher level of polymorphism (158 bands) than ISSR (92 bands). Clustering of genotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. Six clades can be recognized within Galium, which mostly corroborate, but also partly contradict, traditional groupings. UPGMA-based dendrogram showed a close relationship between members of section Leiogalium with G. verum and G. humifusum (sect. Galium), and G. angustifolium (sect. Lophogalium). Principal coordinated analysis, however, showed some minor differences with UPGMA-based dendrograms. The more apomorphic groups of Galium form the section Leiogalium clade including the perennial sections Galium, Lophogalium, Jubogalium, Hylaea and Leptogalium as well as the annual section Kolgyda. The remaining taxa of Galium are monophyletic.

scholarly journals Genetic Similarity of Commerson’s Anchovy across Segara Anakan Cilacap Assessed Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Jurnal Biota ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42-50
Author(s):  
Muhammad Khoerol Anam ◽  
Adi Amurwanto ◽  
Kusbiyanto Kusbiyanto ◽  
Hendro Pramono ◽  
M Husein Sastranegara ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Genetic Differences ◽  
Population Genetic Study ◽  
Randomly Amplified Polymorphic Dna ◽  
High Genetic Diversity ◽  
Impressive Result

Segara Anakan areas can be divided into three different regions according to their salinity. Salinity differences suggested that Commerson’s anchovy population in that area can be divided into three subpopulations due to genetic differences. Genetic differences among subpopulation can be assessed through a population genetic study using random amplified polymorphic DNA. This study aims to evaluate the genetic variation and differences of Commerson's anchovy (Stolephorus commersonnii) collected at three different water salinities in Segara Anakan estuary Cilacap Indonesia. Total genomic DNA was isolated using the Chelex method. Genetic diversity and differences were assessed using RAPD markers and were analyzed statistically using an analysis of molecular variance, as implemented in Arlequin software.  The results showed that high genetic diversity was observed within the subpopulations. However, no significant genetic differences were observed among subpopulations which indicate genetic similarity. A high number of offspring are likely to cause high genetic variation within subpopulations.  Adult and larvae migration is the cause of genetics similarity across Segara Anakan. Another impressive result is that water salinity did not affect the genetic characteristic of Commerson,s anchovy. Genetic similarity of Commerson’s anchovy indicates that Segara Anakan forms a single genetic conservation unit.

Genetic evidence that butternut canker was recently introduced into North America

1999 ◽  
Vol 77 (6) ◽  
pp. 783-785 ◽  
Author(s):  
Glenn R Furnier ◽  
Allison M Stolz ◽  
Raka M Mustaphi ◽  
Michael E Ostry
Keyword(s):  
Genetic Variation ◽  
North America ◽  
Rapd Markers ◽  
Genetic Evidence ◽  
Sexual Stage ◽  
Randomly Amplified Polymorphic Dna ◽  
Native Range ◽  
Canker Disease ◽  
Juglans Cinerea ◽  
Butternut Canker

Butternut (Juglans cinerea) is seriously threatened by a canker disease caused by Sirococcus clavigignenti-juglandacearum, a fungus with no known sexual stage. This pathogen was first reported in 1967 and is now found throughout the native range of butternut, suggesting that it was introduced into North America. We used randomly amplified polymorphic DNA (RAPD) markers to examine genetic variation in S. clavigignenti-juglandacearum over a large portion of the native range of butternut. The 55 RAPD fragments were all found in all 86 isolates of the fungus. This complete monomorphism is consistent with the fungus having been introduced into North America as a single isolate and suggests that if resistant butternut genotypes are found, the pathogen will not likely be able to rapidly evolve genotypes that can attack them.Key words: Sirococcus clavigignenti-juglandacearum, Juglans cinerea, RAPD, randomly amplified polymorphic DNA.

scholarly journals Genetic Analyses of Heliconia Species and Cultivars with Randomly Amplified Polymorphic DNA (RAPD) Markers

1998 ◽  
Vol 123 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Prakash P. Kumar ◽  
Janice C.K. Yau ◽  
Chong Jin Goh
Keyword(s):  
Genetic Variation ◽  
Phylogenetic Tree ◽  
Dna Markers ◽  
Genetic Background ◽  
Rapd Markers ◽  
Cultivar Identification ◽  
Polyacrylamide Gels ◽  
Randomly Amplified Polymorphic Dna ◽  
Genetic Analyses ◽  
Morphological Differences

Many Heliconia species are polymorphic with a large number of cultivars. Cultivar identification has been primarily based on morphological differences of the flowers and inflorescences. A protocol was developed to extract DNA from Heliconia leaves and to analyze genetic variation using RAPD. The percentage genetic similarities among Heliconia species, cultivars, and hybrids were determined. Data from 11 primers indicated that the RAPD technique is useful in distinguishing species and cultivars of Heliconia. Using a single 10-mer primer (OPA 18), we were able to generate distinct RAPD profiles for 16 cultivars of H. psittacorum L.f. grown at the Jurong BirdPark, Singapore, a Heliconia Society International depository. Hence, the characteristic profiles generated by RAPD may be used as additional DNA markers for classifying different species and cultivars of Heliconia. The phylogenetic tree derived from the RAPD data showed that all the 16 cultivars examined are closely related to each other, thus providing the first genetic evidence that this large group of cultivars has a common genetic background. Moreover, two triploid cultivars of H. psittacorum—`Iris' and `Petra'—showed identical RAPD profiles with 10 different primers in agarose and polyacrylamide gels, suggesting that they are of the same genotype.

scholarly journals 090 Identification and Genetic Relatedness among Pecan Cultivars Detected by Randomly Amplified Polymorphic DNA (RAPD) Analysis

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 404B-404
Author(s):  
Patrick J. Conner ◽  
Bruce W. Wood
Keyword(s):  
Cluster Analysis ◽  
Genetic Variation ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Genetic Relatedness ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Genetic Distances ◽  
Carya Illinoinensis ◽  
Randomly Amplified Polymorphic Dna

Genetic variation among pecan [Carya illinoinensis (Wangenh.) K. Koch] cultivars was studied using randomly amplified polymorphic DNA (RAPD) markers. Using a combination of primers, a unique fingerprint was produced for each of the pecan genotypes studied. The genetic relatedness between 44 cultivars was estimated using more than 100 RAPD markers. Genetic distances based on the simple matching coefficient varied from 0.91 to 0.59. The phenetic dendogram developed from cluster analysis showed relatively weak grouping association. However, cultivars with known pedigrees usually grouped with at least one of the parents and genetic similarity estimates appear to agree with known genetic relationships. Using RAPD information in determining genetic relationships among pecan cultivars with unknown or questionable pedigrees and the integration of that knowledge into the breeding program is discussed.

scholarly journals Population genetic variation analysis of bitter gourd wilt caused by Fusarium oxysporum f. sp. momordicae in China using inter simple sequence repeats (ISSR) molecular markers

2021 ◽  
Author(s):  
Rui Zang ◽  
Ying Zhao ◽  
Kangdi Guo ◽  
Kunqi Hong ◽  
Huijun Xi ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Genetic Differentiation ◽  
Fusarium Oxysporum ◽  
Diversity Index ◽  
Gene Diversity ◽  
Pcr Amplification ◽  
Bitter Gourd ◽  
Diseased Plant ◽  
Variation Analysis ◽  
Group I

AbstractBitter gourd wilt caused by Fusarium oxysporum f. sp. momordicae (FOM) is a devastating crop disease in China. A total of 173 isolates characteristic of typical Fusarium oxysporum with abundant microconidia and macroconidia on white or ruby colonies were obtained from diseased plant tissues. BLASTn analysis of the rDNA-ITS of the isolates showed 99% identity with F. oxysporum species. Among the tested isolates, three were infectious toward tower gourd and five were pathogenic to bottle gourd. However, all of the isolates were pathogenic to bitter gourd. For genetic differences analysis, 40 ISSR primers were screened and 11 primers were used for ISSR-PCR amplification. In total, 127 loci were detected, of which 76 were polymorphic at a rate of 59.84%. POPGENE analysis showed that Nei’s gene diversity index (H) and Shannon’s information index (I) were 0.09 and 0.15, respectively, which indicated that the genetic diversity of the 173 isolates was low. The coefficient of gene differentiation (Gst = 0.33 > 0.15) indicated that genetic differentiation was mainly among populations. The strength of gene flow (Nm = 1.01 > 1.0) was weak, indicating that the population differentiation caused by gene drift was blocked to some degree. The dendrogram based on ISSR markers showed that the nine geographical populations were clustered into two groups at the threshold of genetic similarity coefficient of 0.96. The Shandong and Henan populations were clustered into Group I, while the Guangdong, Hainan, Guangxi, Fujian, Jiangxi, and Hubei populations constituted Group II. Results of the genetic variation analysis showed that the Hunan and Guangxi populations had the highest degree of genetic differentiation, while the Hubei population had the lowest genetic differentiation. Our findings enrich the knowledge of the genetic variation characteristics of FOM populations with the goal of developing effective disease-management programs and resistance breeding programs.

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