BIOPESTICIDE POTENTIAL OF (7R)-TRANS, TRANS-NEPETALACTONE AND CIS-LACHNOPHYLLUM ESTER IN CONTROL OF MUSTARD APHID, LIPAPHIS ERYSIMI (KALT.)

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Vinod Kumar ◽  
Chandra Shekhar Mathela ◽  
Geeta Tewari ◽  
Ajay Kumar Pandey
Keyword(s):  
Essential Oils ◽  
Insecticidal Activity ◽  
2D Nmr ◽  
Positive Control ◽  
Lipaphis Erysimi ◽  
Acetylenic Compound ◽  
Mustard Aphid ◽  
Synthetic Pesticide ◽  
Natural Pesticides ◽  
Erigeron Annuus

(7R)-trans, trans-nepetalactone; a monoterpene iridoid (1) and acetylenic compound named cis-lachnophyllum ester (2) were isolated from the essential oils of Nepeta elliptica and Erigeron annuus, respectively and characterized using a combination of their spectral data  (1D-and 2D-NMR, MS, IR). Compound 1 and 2 were tested for biopesticide activity against mustard aphid, Lipaphis erysimi (Kalt.). Compound 1 exhibited high insecticidal activity towards L. erysimi with LC50 values of 2.18 and 2.73 mg/mL; LT50 values of 15.24 and 17.18 h. Compound 2 also displayed significant insecticidal activity having LC50 values of 0.85 and 4.70 mg/mL; LT50 values of 13.25 and 26.2 h. The activity of compounds 1 and 2 were comparable with synthetic pesticide, monocrotophos used as positive control and thus has potential as natural pesticides for use in economically important crops.

scholarly journals Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium

Toxins ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 598 ◽  
Author(s):  
Shixing Zhou ◽  
Caixia Wei ◽  
Chi Zhang ◽  
Caixia Han ◽  
Nigora Kuchkarova ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Insecticidal Activity ◽  
Petri Dish ◽  
Northwest China ◽  
Amaranthus Retroflexus ◽  
Diffusion Method ◽  
First Report ◽  
Ic50 Values ◽  
Natural Pesticides

The present investigation studied the chemical composition of the essential oils extracted from Dracocephalum integrifolium Bunge growing in three different localities in northwest China and evaluated the phytotoxic, antimicrobial and insecticidal activities of the essential oils as well as their major constituents, i.e., sabinene and eucalyptol. GC/MS analysis revealed the presence of 21–24 compounds in the essential oils, representing 94.17–97.71% of the entire oils. Monoterpenes were the most abundant substances, accounting for 85.30–93.61% of the oils; among them, sabinene (7.35–14.0%) and eucalyptol (53.56–76.11%) were dominant in all three oils, which occupied 67.56–83.46% of the total oils. In general, phytotoxic bioassays indicated that the IC50 values of the oils and their major constituents were below 2 μL/mL (1.739–1.886 mg/mL) against Amaranthus retroflexus and Poa annua. Disc diffusion method demonstrated that the oils and their major constituents possessed antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Saccharomyces cerevisiae, and Candida albicans, with MIC values ranging from 5–40 μL/mL (4.347–37.712 mg/mL). The oils, sabinene and eucalyptol also exhibited significant pesticidal activity, with the mortality rates of Aphis pomi reaching 100% after exposing to 10 μL oil/petri dish (8.694–9.428 mg/petri dish) for 24 h. To the best of our knowledge, this is the first report on the chemical composition, phytotoxic, antimicrobial and insecticidal activity of the essential oils extracted from D. integrifolium; it is noteworthy to mention that this is also the first report on the phytotoxicity of one of the major constituents, sabinene. Our results imply that D. integrifolium oils and sabinene have the potential value of being further exploited as natural pesticides.

scholarly journals Isolation of eudesmane type sesquiterpene ketone from Prangos heyniae H.Duman & M.F.Watson essential oil and mosquitocidal activity of the essential oils

2018 ◽  
Vol 16 (1) ◽  
pp. 453-467 ◽  
Author(s):  
Gülmira Özek ◽  
Erdal Bedir ◽  
Nurhayat Tabanca ◽  
Abbas Ali ◽  
Ikhlas A. Khan ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
2D Nmr ◽  
Positive Control ◽  
System Structure ◽  
Gas Chromatography Mass Spectrometry ◽  
Flame Ionization ◽  
Fraction Collector ◽  
Mosquitocidal Activity ◽  
One Step

AbstractIn the present work, an endemic species Prangos heyniae collected in four locations from Turkey was subjected to hydrodistillation in Clevenger type apparatus to obtain the essential oils (EO1-4). The gas-chromatography/mass spectrometry (GC/MS) and gas-chromatography-flame ionization detector (GC/FID) analyses showed that the EOs were rich in sesquiterpenes, germacrene D (10.3-12.1%), β-bisabolene (14.4%), kessane (26.9%), germacrene B (8.2%), elemol (3.4-46.9%), β-bisabolenal (1.4-70.7%), β-bisabolenol (8.4%) and an eudesmane type sesquiterpene (1) (16.1%) with [M+218]. This unidentified compound (1) was isolated in a rapid one-step manner with >95.0% purity using Preparative Capillary Gas Chromatography (PCGC) with an HP Innowax column connected to a Preparative Fraction Collector (PFC) system. Structure determination was accomplished from 1D- and 2D-NMR spectroscopic data which determined a new eudesmane type sesquiterpene, 3,7(11)-eudesmadien-2-one (1). Using a biting deterrent bioassay, the mean proportion not biting (PNB) values of the P. heyniae EO1-4 were 0.88 for EO1 and 0.80 for EO2 which were similar to the positive control DEET (N,N-diethyl-3-methylbenzamide). The EO3 and EO4 had lower PNB values of 0.64 and 0.44, respectively. P. heyniae EO1-4 showed good larvicidal activity at 125 and 62.5 ppm whereas EO1-3 were slightly less effective at the dose of 31.25 ppm and EO4 was not active at 31.25 ppm against 1st instar Aedes aegypti.

scholarly journals Bio-Efficacy Of Some Plant Leaf Extracts Against Mustard Aphid, Lipaphis Erysimi Kalt. On Indian Mustard, Brassica Juncea

2010 ◽  
Vol 50 (2) ◽  
pp. 130-132 ◽  
Author(s):  
Arshad Ali ◽  
Parvez Rizvi ◽  
Farmanur Khan
Keyword(s):  
Brassica Juncea ◽  
Insecticidal Activity ◽  
Indian Mustard ◽  
Lipaphis Erysimi ◽  
Calotropis Procera ◽  
Leaf Extracts ◽  
Argemone Mexicana ◽  
Mustard Aphid ◽  
Plant Leaf ◽  
Tagetes Minuta

Bio-Efficacy Of Some Plant Leaf Extracts Against Mustard Aphid,Lipaphis ErysimiKalt. On Indian Mustard,Brassica JunceaThe bio-efficacy of four plant leaf extractsviz., apple of sodom,Calotropis proceraAiton; Mexican poppy,Argemone mexicanaLinnaeus; Mexican marigold,Tagetes minutaLinnaeus and Indian neem,Azadirachta indicawere tested against mustard aphid,Lipaphis erysimion Indian mustard,Brassica juncea. The highest per cent aphid reduction during first, second and third spray were 28.79, 40.52 and 59.32 at 1 : 10 g/ml; 34.70, 44.49 and 66.14 at 1 : 5 g/ml and 53.88, 64.84 and 100.00 at 1 : 2.5 g/ml with Indian neem. However, Mexican marigold was also effective at highest concentration (1 : 2.5 g/ml) and reduced 96.38 per centL. erysimi. All the treatments of plant leaf extracts showed insecticidal activity, but Indian neem followed by Mexican marigold reduced the aphid population to a great extent.

scholarly journals Insecticidal Pregnane Glycosides from the Root Barks of Periploca sepium

2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101
Author(s):  
Renfeng Li ◽  
Ximei Zhao ◽  
Baojun Shi ◽  
Shaopeng Wei ◽  
Jiwen Zhang ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
2D Nmr ◽  
Positive Control ◽  
Botanical Pesticides ◽  
Natural Sources ◽  
Lead Compounds ◽  
Pregnane Glycoside ◽  
Periploca Sepium ◽  
Insecticidal Activities

To explore novel lead compounds for botanical pesticides from natural sources, a new pregnane glycoside (periplocoside) P2 as well as its isomer, a known pregnane glycoside P1, were isolated from the root barks of Periploca sepium using a bioactivity-guided method, and their structures were confirmed by 1D NMR, 2D NMR, IR, ESI-MSn and HRMS. Their insecticidal activities were evaluated against 3rd instar larvae of M. separata, and the results indicated that P2 exhibited excellent insecticidal activity with LC50 values of 2.9 and 2.2 mg/mL after 24 and 48 h, respectively, much lower than those of the positive control celangulin-V (24.7 and 21.0 mg/mL after 24 and 48 h, respectively). This work demonstrated that pregnane glycosides from Periploca sepium could be promising lead compounds for developing botanical pesticides urgently needed in agriculture.

scholarly journals Insecticidal Activity of Vegetable Oils against Mustard aphid, Lipaphis erysimi Kalt., under Field Condition

2006 ◽  
Vol 55 (5) ◽  
pp. 227-231 ◽  
Author(s):  
Dheeraj MISHRA ◽  
A.K. SHUKLA ◽  
A.K. DUBEY ◽  
A.K. DIXIT ◽  
K. SINGH
Keyword(s):  
Vegetable Oils ◽  
Insecticidal Activity ◽  
Field Condition ◽  
Lipaphis Erysimi ◽  
Mustard Aphid

Evaluation of antioxidant and antimicrobial activity of Ziziphora ‎clinopodiodes essential oil eluted at different times

2020 ◽  
Vol 16 ◽  
Author(s):  
Mojgan Alizadeh ◽  
Akram Arianfar ◽  
Ameneh Mohammadi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antioxidant Activities ◽  
Positive Control ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Citronellic Acid ◽  
Antimicrobial And Antioxidant Activity

Objective: Ziziphora clinopodioides is an edible medicinal plant belongs to the Labiatae family that widespread all over Iran. It used as culinary and also in cold and cough treatments in Iran. The aim of present work was to evaluate the effect of different timeframes during the hydrodistillation on essential oil composition, antimicrobial and antioxidant activity. Materials and Methods: The essential oil of Z. clinopodiodes was extracted via hydrodistillation with Clevenger apparatus. The fractions of essential oil were captured at 6 times from the beginning of the distillation: (10, 20, 60, 120, 180 and 240 min). The fractions of essential oil were analyzed by GC/MS and their antibacterial, antifungal and antioxidant activities were studied by Disk - well diffusion and DPPH methods respectively. Results: Six distillation times and whole essential oil were captured during the hydrodistillation. Essential oil yield dropped off significantly during distillation progressed (1.0% for 10 min and 0.025 for 240 min). 1,8 Cineol, Isomenthone, Pulegone, Piperitenone and Citronellic acid were major compounds in fractions and they were affected by distillation times. Pulegone was major compound in all of essential oils. In antioxidant activity assay, whole essential oil was stronger than was stronger than positive control and fractions of essential oil, because of higher levels of Isomenthone, Piperitenone and Citronellic acid. Strongest antimicrobial activity against S. aureus, E. coli and C. albicans was observed from 10 min fraction. Conclusion: Our results indicated that distillation time can create essential oils with specific properties and we can achieve to more efficient essential oil in short times.

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