scholarly journals Detection of Clostridium tyrobutyricum using cultivation and biochemical methods and polymerase chain reaction

2007 ◽  
Vol 55 (5) ◽  
pp. 23-28
Author(s):  
Radka Burdychová ◽  
Pavla Sládková
Keyword(s):  
Butyric Acid ◽  
Raw Milk ◽  
Rapid Screening ◽  
Clostridium Tyrobutyricum ◽  
Acid Fermentation ◽  
Cultivation Technique ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Polymyxine B ◽  
Spore Forming Bacteria

Anaerobic spore-forming bacteria of the genusClostridiumare commonly present in raw milk and some milk products. Their spores can survive pasteurization and can provoke so called late blowing defect in cheese caused by butyric acid fermentation. The only species of the genusClostridiumthat is able to provoke late blowing isClostridium tyrobutyricum.In this work, two cultivation methods for detection of butyric acid producing clostridia in raw and pasteurized milk and in cheese samples were compared. The results show that tube method is suitable for route identification (in concentration 102CFU/ml or /g) of clostridia in milk and cheese. The standard cultivation technique is suitable for more sensitive identification (10 CFU/ml or /g). All presumptive colonies grown anaerobically on selective RCM agar with polymyxine B (500 μg/ml) were classified to be of speciesClostridium tyrobutyricumusing PCR only. The confirmation using API tests were different in 50 % cases. The results show, that described PCR method is suitable for rapid screening of the presence ofClostridium tyrobutyricumin milk and cheese. PCR from one colony is possible to use for the analysis.

Isolation and detection of Clostridium tyrobutyricum cells in semi-soft and hard cheeses using the polymerase chain reaction

1997 ◽  
Vol 64 (2) ◽  
pp. 311-314 ◽  
Author(s):  
LIEVE HERMAN ◽  
JAN DE BLOCK ◽  
ROLAND VAN RENTERGHEM
Keyword(s):  
Polymerase Chain Reaction ◽  
Butyric Acid ◽  
Causative Agent ◽  
Anaerobic Bacterium ◽  
Clostridium Tyrobutyricum ◽  
Chain Reaction ◽  
Acid Fermentation ◽  
Polymerase Chain ◽  
Considerable Loss ◽  
Butyric Acid Fermentation

Butyric acid fermentation in brine salted, semi-soft and hard cheeses (late blowing) can create considerable loss of product. Clostridium tyrobutyricum, a Gram-positive, sporeforming, anaerobic bacterium, has been identified as the causative agent (Klijn et al. 1995).

scholarly journals Butyric Acid Fermentation in Xylose and Glucose by Clostridium tyrobutyricum

BioResources ◽  
2017 ◽  
Vol 12 (2) ◽  
Author(s):  
Guanghong Luo ◽  
Ling Zhang ◽  
Tianren Chen ◽  
Wenqiao Yuan ◽  
Yingxi Geng
Keyword(s):  
Butyric Acid ◽  
Clostridium Tyrobutyricum ◽  
Acid Fermentation ◽  
Butyric Acid Fermentation

Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses

2009 ◽  
Vol 100 (13) ◽  
pp. 3403-3409 ◽  
Author(s):  
Ling Jiang ◽  
Jufang Wang ◽  
Shizhong Liang ◽  
Xiaoning Wang ◽  
Peilin Cen ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Clostridium Tyrobutyricum ◽  
Acid Fermentation ◽  
Cane Molasses ◽  
Fibrous Bed Bioreactor ◽  
Butyric Acid Fermentation

scholarly journals Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran

2020 ◽  
Vol 8 (3) ◽  
pp. 76-79
Author(s):  
Mahtab Hamidpour ◽  
Saman Mahdavi
Keyword(s):  
Bacillus Cereus ◽  
Bacterial Contamination ◽  
Dairy Product ◽  
Foodborne Pathogen ◽  
Food Poisoning ◽  
Raw Milk ◽  
Specific Primers ◽  
Different Types ◽  
Pcr Method ◽  
Polymerase Chain

Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.

scholarly journals Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs

2021 ◽  
Author(s):  
Manjusha Lekshmi ◽  
Sanath H. Kumar ◽  
Kooloth Valappil Rajendran ◽  
Binaya Bhusan Nayak
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Rapid Screening ◽  
Bivalve Molluscs ◽  
Chain Reaction ◽  
Reverse Transcription Pcr ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Rna Polymerase Gene ◽  
Single Set

Abstract Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII.

Investigation of duck plague virus in hoar areas of Bangladesh

2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  
Keyword(s):  
Cytopathic Effect ◽  
Clinical Sample ◽  
Chorioallantoic Membrane ◽  
Clinical Samples ◽  
Isolation And Identification ◽  
Duck Plague Virus ◽  
Pcr Method ◽  
Polymerase Chain ◽  
The Individual ◽  
Free Ranging

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78

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