scholarly journals OPTIMIZATION OF PROCESS PARAMETERS FOR CULTIVATION OF BACTERIOPHAGES PROMISING FOR BIOLOGICAL CONTROL OF PHYTOPATHOGENIC BACTERIA OF GENUS PSEUDOMONAS

2021 ◽  
Author(s):  
T.A. Pilipchuk ◽  
A.D. Gerasimovich ◽  
I.N. Ananyeva ◽  
E.I. Kolomiets
Keyword(s):  
Biological Control ◽  
Pseudomonas Syringae ◽  
Process Parameters ◽  
Fish Meal ◽  
Aeration Rate ◽  
Lytic Activity ◽  
Agitation Rate ◽  
Phytopathogenic Bacteria ◽  
Optimization Of Process Parameters ◽  
Optimized Conditions

Cultural parameters of bacteriophages screened for lytic activity toward host bacterial strain P. helmanticensis BIM В-582 D were investigated. The following conditions were shown to be optimal for cultivation of indicator culture and it’s lysis by Pseudomonas phages BIM ВV-45 D, BIM ВV-46 D, BIM ВV-47 D, BIM ВV-50 D, BIM ВV-53 D, BIM ВV-61 D in laboratory fermenters ANKUM-3M: nutrient medium – fish meal hydrolysate broth, temperature – 28°C, stirrer agitation rate – 160 rpm, aeration rate – 1 l air/ l medium per min. Biopreparation produced under optimized conditions and composed of six afore-mentioned phages display high lytic activity both in regard to the host strain and to phytopathogenic bacteria Pseudomonas syringae.

Optimization of Process Parameters for Production of Pectinase using Bacillus Subtilis MF447840.1

2019 ◽  
Vol 13 (1) ◽  
pp. 69-73 ◽  
Author(s):  
Ram Balak Mahto ◽  
Mukesh Yadav ◽  
Soumya Sasmal ◽  
Biswnath Bhunia
Keyword(s):  
Bacillus Subtilis ◽  
Process Parameters ◽  
Volume Ratio ◽  
Optimum Process ◽  
Agitation Rate ◽  
Fermentation Condition ◽  
Optimization Of Process Parameters ◽  
Food And Beverage ◽  
Pectinase Production ◽  
Sterile Product

Background: Pectinase enzyme has immense industrial prospects in the food and beverage industries. </P><P> Objective: In our investigation, we find out the optimum process parameters suitable for better pectinase generation by Bacillus subtilis MF447840.1 using submerged fermentation. </P><P> Method: 2% (OD600 nm = 0.2) of pure Bacillus subtilis MF447840.1 bacterial culture was inoculated in sterile product production media. The production media components used for this study were 1 g/l of pectin, 2 g/l of (NH4)2SO4, 1 g/l of NaCl, 0.25 g/l of K2HPO4, 0.25 g/l of KH2PO4 and 1 g/l of MgSO4 for pectinase generation. We reviewed all recent patents on pectinase production and utilization. The various process parameters were observed by changing one variable time method. </P><P> Results: The optimum fermentation condition of different parameters was noticed to be 5% inoculums, 25% volume ratio, temperature (37°C), pH (7.4) and agitation rate (120 rpm) following 4 days incubation. </P><P> Conclusion: Maximum pectinase generation was noticed as 345 ± 12.35 U following 4 days incubation.

scholarly journals BACTERIOPHAGE PREPARATION ENGINEERING FOR BIOCONTROL OF PSEUDOMONAS SYRINGAE IN CROP SCIENCE

2020 ◽  
pp. 130-137
Author(s):  
D. А. Vasiliev ◽  
◽  
А.К. Bekkalieva ◽  
N. А. Feoktistova ◽  
Е. V. Suldina ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Biocontrol Agent ◽  
Molecular Genetic ◽  
Living Cells ◽  
Lytic Activity ◽  
Cultivated Plants ◽  
Phage Lysate ◽  
Genetic Characteristics ◽  
Phytopathogenic Bacteria ◽  
Crop Science

Phytopathogenic bacteria Pseudomonas syringae cause diseases of many cultivated plants, causing tumor neoformation, rot, chlorosis, necrosis, etc . The advanced biological mean to control bacteriosis in crop science is bacteriophages. In this work full biological characteristic of 8 bacteriophages is shown, active according to Pseudomonas syringae. The studied phages formed similar negative colonies- clear, rounded, in diameter of 5-9 mm. Lytic actifity of phages Pseudomonas syringae by Appelman from 10-4 to 10-8; by Gratia from 1,0±0,1×106 to 2,0±0,1×109 (BFU/ml). Bacteriophages Ps.s-7 UlGAU, Ps.s-13 UlGAU and Ps.s-27 UlGAU did not change lytic activity when storing in fridge during 12 months. Lytic activity of phages Ps.s-1 UlGAU, Ps.s-8, Ps.s-15 UlGAU, Ps.s-30 UlGAU, Ps.s-77 UlGAU in the same conditions fell within 1-2 orders. Spectrum of lytic activity of phages varied from 21,4% (Ps.s-13 UlGAU) to 85,7% (Ps.s-7 UlGAU, Ps.s-27 UlGAU). The study of phage specificity on 15 species og heterologous cultures showed that phages are specific for Pseudomonas syringae. Phages are moderately stable to heating and lose activity during 30 –minute temperature effect above 62°С. The optimal way to relieve phage lysate from living cells of Pseudomonas syringae was thrichloromethane at a ratio of 10:1and temporal exposition 45 minutes. On the basis of obtained data we determined capacity of each bacteriophage for the use as biocontrol agent. For further research for the study of molecular genetic characteristics we selected advanced strains of phages Pseudomonas syringae - Ps.s-7 UlGAU и Ps.s-27 UlGAU.

Recent trends in approaches for optimization of process parameters for the production of microbial cellulase from wastes

2021 ◽  
Author(s):  
Dibyajit Lahiri ◽  
Moupriya Nag ◽  
Dipro Mukherjee ◽  
Sayantani Garai ◽  
Ritwik Banerjee ◽  
...  
Keyword(s):  
Process Parameters ◽  
Optimization Of Process Parameters ◽  
Recent Trends

scholarly journals Taguchi optimization and scale up of xylanase from Bacillus licheniformis isolated from hot water geyser

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Girisha Malhotra ◽  
Shilpa S. Chapadgaonkar
Keyword(s):  
Process Parameters ◽  
Wheat Bran ◽  
Scale Up ◽  
Industrial Applications ◽  
Hot Water ◽  
Optimization Strategy ◽  
Taguchi Optimization ◽  
Xylanase Production ◽  
Alkali Tolerance ◽  
Optimized Conditions

Abstract Background Xylanase is one of the widely applied industrial enzymes with diverse applications. Thermostability and alkali tolerance are the two most desirable qualities for industrial applications of xylanase. In this paper, we reveal the statistical Taguchi optimization strategy for maximization of xylanase production. The important process parameters pH, temperature, concentration of wheat bran, and concentration of yeast extract were optimized using the Taguchi L8 orthogonal array where the 4 factors were considered at 2 levels (high and low). Results The optimized conditions given by model were obtained as follows: (i) pH 6, (ii) culture temperature 35 °C, (iii) concentration of xylan 2% w/v, (iv) concentration of wheat bran 2.5% w/v. The production was scaled upto 2.5 L bioreactor using optimized process parameters. A high xylanase titer of 400 U/ml could be achieved in less than 60 h of culture in the reactor. Conclusion Optimization was successful in achieving about threefold increase in the yield of xylanase. The optimized conditions resulted in a successful scale up and enhancement of xylanase production.

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