scholarly journals Taguchi optimization and scale up of xylanase from Bacillus licheniformis isolated from hot water geyser

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Girisha Malhotra ◽  
Shilpa S. Chapadgaonkar
Keyword(s):  
Process Parameters ◽  
Wheat Bran ◽  
Scale Up ◽  
Industrial Applications ◽  
Hot Water ◽  
Optimization Strategy ◽  
Taguchi Optimization ◽  
Xylanase Production ◽  
Alkali Tolerance ◽  
Optimized Conditions

Abstract Background Xylanase is one of the widely applied industrial enzymes with diverse applications. Thermostability and alkali tolerance are the two most desirable qualities for industrial applications of xylanase. In this paper, we reveal the statistical Taguchi optimization strategy for maximization of xylanase production. The important process parameters pH, temperature, concentration of wheat bran, and concentration of yeast extract were optimized using the Taguchi L8 orthogonal array where the 4 factors were considered at 2 levels (high and low). Results The optimized conditions given by model were obtained as follows: (i) pH 6, (ii) culture temperature 35 °C, (iii) concentration of xylan 2% w/v, (iv) concentration of wheat bran 2.5% w/v. The production was scaled upto 2.5 L bioreactor using optimized process parameters. A high xylanase titer of 400 U/ml could be achieved in less than 60 h of culture in the reactor. Conclusion Optimization was successful in achieving about threefold increase in the yield of xylanase. The optimized conditions resulted in a successful scale up and enhancement of xylanase production.

scholarly journals Dynamic modeling of the isoamyl acetate reactive distillation process

2017 ◽  
Vol 19 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Syed Sadiq Ali ◽  
Sk Safdar Hossain ◽  
Mohammad Asif
Keyword(s):  
Dynamic Modeling ◽  
Process Parameters ◽  
Reactive Distillation ◽  
Scale Up ◽  
Isoamyl Acetate ◽  
Industrial Applications ◽  
Process Conditions ◽  
Whole Process ◽  
Product Flow ◽  
The Stability

Abstract The cost-effectiveness of reactive distillation (RD) processes makes them highly attractive for industrial applications. However, their preliminary design and subsequent scale-up and operation are challenging. Specifically, the response of RD system during fluctuations in process parameters is of paramount importance to ensure the stability of the whole process. As a result of carrying out simulations using Aspen Plus, it is shown that the RD process for isoamyl acetate production was much more economical than conventional reactor distillation configuration under optimized process conditions due to lower utilities consumption, higher conversion and smaller sizes of condenser and reboiler. Rigorous dynamic modeling of RD system was performed to evaluate its sensitivity to disturbances in critical process parameters; the product flow was quite sensitive to disturbances. Even more sensitive was product composition when the disturbance in heat duties of condenser or reboiler led to a temperature decrease. However, positive disturbance in alcohol feed is of particular concern, which clearly made the system unstable.

scholarly journals Influence of High-Productivity Process Parameters on the Surface Quality and Residual Stress State of AISI 316L Components Produced by Directed Energy Deposition

2021 ◽  
Author(s):  
Gabriele Piscopo ◽  
Alessandro Salmi ◽  
Eleonora Atzeni
Keyword(s):  
Surface Roughness ◽  
Residual Stresses ◽  
Surface Quality ◽  
Process Parameters ◽  
Energy Deposition ◽  
Industrial Applications ◽  
High Productivity ◽  
Directed Energy Deposition ◽  
Productivity Process ◽  
Directed Energy

AbstractThe production of large components is one of the most powerful applications of laser powder-directed energy deposition (LP-DED) processes. High productivity could be achieved, when focusing on industrial applications, by selecting the proper process parameters. However, it is of crucial importance to understand the strategies that are necessary to increase productivity while maintaining the overall part quality and minimizing the need for post-processing. In this paper, an analysis of the dimensional deviations, surface roughness and subsurface residual stresses of samples produced by LP-DED is described as a function of the applied energy input. The aim of this work is to analyze the effects of high-productivity process parameters on the surface quality and the mechanical characteristics of the samples. The obtained results show that the analyzed process parameters affect the dimensional deviations and the residual stresses, but have a very little influence on surface roughness, which is instead dominated by the presence of unmelted particles.

scholarly journals Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

2015 ◽  
Vol 176 (1) ◽  
pp. 317-319 ◽  
Author(s):  
N. Pérez-Rodríguez ◽  
F. Oliveira ◽  
B. Pérez-Bibbins ◽  
I. Belo ◽  
A. Torrado Agrasar ◽  
...  
Keyword(s):  
Solid State ◽  
Filamentous Fungi ◽  
Solid State Fermentation ◽  
Scale Up ◽  
Horizontal Tube ◽  
Xylanase Production

Preparation of PPy Nanowires and its Amperometric Response to Nitrate

2007 ◽  
Vol 336-338 ◽  
pp. 2154-2156
Author(s):  
Xiu Ling Zhang ◽  
Ji Xiao Wang ◽  
Zhi Wang ◽  
Shi Chang Wang
Keyword(s):  
Process Parameters ◽  
Monomer Concentration ◽  
Modified Electrodes ◽  
Buffer Solution ◽  
Amperometric Response ◽  
Nitrate Ions ◽  
Pyrrole Monomer ◽  
Electrocatalytic Effect ◽  
Template Free ◽  
Optimized Conditions

Polypyrrole(PPy) nanowires were prepared by template-free method electrochemically. The effect of process parameters on the morphology of PPy was investigated. The electrocatalytic effect of PPy nanowire modified electrodes was characterized by its amperometetric response toward nitrate ions. The experimental results indicated that the process parameters of the modified electrodes such as concentration of pyrrole monomer, concentration of support electrolyte (LiClO4), and the pH of buffer solution have significant effects on the diameter the length and smooth of PPy nanowires, and thus have obviously effects on electroreduction current density of nitrate ions. Under the optimized conditions, the PPy nanowire modified electrodes have high electrocatalytic activity toward nitrate electroreduction.

Application of the Continuous Rheoconversion Process (CRP) to Low Temperature HPDC-Part I: Microstructure

2006 ◽  
Vol 116-117 ◽  
pp. 402-405 ◽  
Author(s):  
Qin Yue Pan ◽  
Stuart Wiesner ◽  
Diran Apelian
Keyword(s):  
Low Temperature ◽  
Scale Up ◽  
Microstructural Characterization ◽  
Industrial Applications ◽  
Primary Phase ◽  
Heat Extraction ◽  
Initial Stage ◽  
Fraction Solid ◽  
Die Castings

The continuous rheoconversion process (CRP) is a novel slurry-on-demand process that was developed at MPI/WPI in 2002. The process is based on a passive liquid mixing technique in which the nucleation and growth of the primary phase are controlled using a specially designed “reactor”. The reactor provides heat extraction, copious nucleation, and forced convection during the initial stage of solidification, thus leading to the formation of globular structures. This paper presents our recent work on the scale-up of the CRP for industrial applications. Specifically, we demonstrate an important application of the CRP to low temperature (low fraction solid) HPDC. In Part I of this paper, we present salient results on microstructural characterization of CRP processed castings vs. conventional die castings.

Comparison between Modelled Influence of GMAW Parameters and Corresponding Mechanical Properties of Group 1 and 2 According to ISO/TR 15608 Steel T Joints

2021 ◽  
Vol 890 ◽  
pp. 17-24
Author(s):  
Aurel Valentin Bîrdeanu ◽  
Alin Constantin Murariu ◽  
Horia Florin Daşcău ◽  
Iuliana Duma
Keyword(s):  
Process Parameters ◽  
High Performance ◽  
Hsla Steel ◽  
Welding Process ◽  
High Strength ◽  
Industrial Applications ◽  
Steel Plates ◽  
Experimental Program ◽  
Welding Parameters ◽  
T Joints

Reproducibility in respect to welded structures realization is one of the main requirements for a wide variety of industrial applications. One of the international tendencies regarding the use of the steel is the replacing, in critical areas, of structural steels with high performance steel, e.g. with HSLA steels. The paper presents the results of a factorial designed experimental program focused on determining mathematical correlations between the GMAW process parameters for T joints of 4mm thick steel plates of structural (S235JR+AR according to SR EN 10025-2) and hot-rolled, high-strength low-alloy (HSLA) steel plates (S420MC according to EN 10025-4), respectively. A comparison between the obtained mathematical correlations that connect the welding parameters and the main mechanical characteristics is presented. The correlations can be used for applying the optimal combination of welding process parameters for realizing the T-joints of welded products.

scholarly journals Cross-Linking of Wheat Bran Arabinoxylan by Fungal Laccases Yields Firm Gels

Processes ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 36 ◽  
Author(s):  
Sanaz Khalighi ◽  
Ralf G. Berger ◽  
Franziska Ersoy
Keyword(s):  
Wheat Bran ◽  
High Water ◽  
Industrial Applications ◽  
Citrus Pectin ◽  
Food Markets ◽  
Pleurotus Pulmonarius ◽  
Gelatin Gels ◽  
Funalia Trogii ◽  
Kosher Food ◽  
Water Holding

The native extractable arabinoxylans (AX) from wheat bran were cross-linked by the commercial laccase C (LccC) and self-produced laccases from Funalia trogii (LccFtr) and Pleurotus pulmonarius (LccPpu) (0.04 U/µg FA, each). Dynamic oscillation measurements of the 6% AX gels demonstrated a storage modulus of 9.4 kPa for LccC, 9.8 kPa for LccFtr, and 10.0 kPa for LccPpu. A loss factor ≤ 0.6 was recorded in the range from 20 to 80 Hz for all three laccases, and remained constant for four weeks of storage, when LccFtr and LccPpu were used. Arabinoxylan gel characteristics, including high water holding capacity, swelling ratio in saliva, and heat resistance indicated a covalently cross-linked network. Neither the mediator compounds caffeic acid and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), nor citrus pectin, enhanced the elastic properties of the gels. Using laccases as an oxidant provided gels with a solid and stable texture, comparable in firmness to traditional gelatin gels. Thus, AX gels can be presented in the vegan, halal, and kosher food markets. They may also find use in pharmaceutical and other industrial applications.

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