Bio-Ferrography to Capture and Separate Polyethylene Wear Debris from Hip Simulator Fluid and Compared with Conventional Filter Method

2005 ◽  
Vol 128 (2) ◽  
pp. 436-441 ◽  
Author(s):  
Donna M. Meyer ◽  
Adam Tillinghast ◽  
Nevan C. Hanumara ◽  
Ana Franco
Keyword(s):  
Experimental Method ◽  
Bovine Serum ◽  
Wear Debris ◽  
Polyethylene Wear ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Filter Method ◽  
Acetabular Cup ◽  
Serum Samples ◽  
Uhmwpe Wear ◽  
Uhmwpe Particle

This paper describes an experimental method, bio-ferrography, to separate ultrahigh molecular weight polyethylene (UHMWPE) wear debris, generated in hip simulators, from bovine serum lubricating fluid. A total of 54 experiments were performed in which an enzyme digestion “cocktail” was developed and used to clean the bovine serum samples of extraneous sugars, proteins, and lipids that interfere with the UHMWPE particle separation. Erbium chloride was used to marginally magnetize particles in the fluid prior to passing through the ferrographic device. The particles were captured and separated from the fluid by traversing the treated serum across a magnetic gap of a bio-ferrograph. Morphology of the captured and separated wear debris was compared with particles from samples of fluid filtered through a paper sieve arrangement with pores of 0.05micrometers in diameter. The UHMWPE wear debris collected using the described experimental method, were found to be between 0.1 and 20micrometers in diameter with spherical and pill-shaped particles. The filtered UHMWPE particles were in the same size range as the debris separated using bio-ferrography. To show that the experimental method captured UHMWPE particles, the spectra of the chemical composition of UHMWPE from an acetabular cup insert of a hip implant and of UHMWPE particles separated using bio-ferrography were compared and found to be the same. To further demonstrate that polyethylene could be captured and separated through the experimental method, manufactured polyethylene microspheres in the diameter range of 3-45micrometers, were captured and separated using the bio-ferrographic process.

Polyethylene Wear Debris From Hip Simulator Fluid Captured and Separated Using Bio-Ferrography

2004 ◽  
Author(s):  
Donna M. Meyer ◽  
Adam Tillinghast ◽  
Nevan C. Hanumara ◽  
Ana Franco
Keyword(s):  
Experimental Method ◽  
Bovine Serum ◽  
Wear Debris ◽  
Polyethylene Wear ◽  
Acetabular Cup ◽  
Serum Samples ◽  
Uhmwpe Wear ◽  
Hip Simulator ◽  
Erbium Chloride ◽  
Uhmwpe Particle

This paper describes an experimental method, Bio-Ferrography, to separate ultra high molecular weight polyethylene (UHMWPE) wear debris, generated in hip simulators, from bovine serum lubricating fluid. A total of 54 experiments were performed in which an enzyme digestion “cocktail” was developed and used to clean the bovine serum samples of extraneous sugars, proteins and lipids that interfere with the UHMWPE particle separation. Erbium chloride was used to marginally magnetize particles in the fluid prior to passing through the ferrographic device. The particles were captured and separated from the fluid by traversing the treated serum across a magnetic gap of a bio-ferrograph. Morphology of the captured and separated wear debris was compared with particles from samples of fluid filtered through a paper sieve arrangement with pores of 0.05 micrometers in diameter. The UHMWPE wear debris collected using the described experimental method, were found to be between 0.1 and 20 micrometers in diameter with spherical and pill-shaped particles. The filtered UHMWPE particles were also in the same size range as the debris separated using bio-ferrography, 0.1 to 20 micrometers. To show that the experimental method captured UHMWPE particles, the spectra of the chemical composition of UHMWPE from an acetabular cup insert of a hip implant and of UHMWPE particles separated using Bio-Ferrography were compared and found to be the same. To further demonstrate that polyethylene could be captured and separated through the experimental method, manufactured polyethylene microspheres in the diameter range of 3 to 45 micrometers, were captured and separated using the bio-ferrographic process.

Ultra-High Molecular Weight Polyethylene Wear Debris Generated in Vivo and in Laboratory Tests; the Influence of Counterface Roughness

1996 ◽  
Vol 210 (1) ◽  
pp. 3-10 ◽  
Author(s):  
J L Hailey ◽  
E Ingham ◽  
M Stone ◽  
B M Wroblewski ◽  
J Fisher
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Wear Debris ◽  
Laboratory Tests ◽  
Polyethylene Wear ◽  
Uhmwpe Wear ◽  
Major Variable ◽  
Ultra High Molecular Weight ◽  
Counterface Roughness

The objective of this study was to investigate the effect of counterface roughness and lubricant on the morphology of ultra-high molecular weight polyethylene (UHMWPE) wear debris generated in laboratory wear tests, and to compare this with debris isolated from explanted tissue. Laboratory tests used UHMWPE pins sliding against stainless steel counterfaces. Both water and serum lubricants were used in conjunction with rough and smooth counterfaces. The lubricants and tissue from revision hip surgery were processed to digest the proteins and permit filtration. This involved denaturing the proteins with potassium hydroxide (KOH), sedimentation of any remaining proteins, and further digestion of these proteins with chromic acid. All fractions were then passed through a 0.2 μm membrane, and the debris examined using scanning electron microscopy. The laboratory studies showed that the major variable influencing debris morphology was counterface roughness. The rougher counter-faces produced larger numbers of smaller particles, with a size range extending below 1 μm. For smooth counterfaces there were fewer of these small particles, and evidence of larger platelets, greater than 10 μm in diameter. Analysis of the debris from explanted tissues showed a wide variation in the particle size distribution, ranging from below 1 μm up to several millimetres in size. Of major clinical significance in relation to osteolysis and loosening is roughening of the femoral components, which may lead to greater numbers of the sub-micron-sized particles.

Influence of gelatin and bovine serum lubricants on ultra-high molecular weight polyethylene wear debris generated in in vitro simulations

2000 ◽  
Vol 214 (5) ◽  
pp. 513-518 ◽  
Author(s):  
J Bell ◽  
A A Besong ◽  
J L Tipper ◽  
E Ingham ◽  
B M Wroblewski ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Bovine Serum ◽  
Wear Debris ◽  
Polyethylene Wear ◽  
In Vitro Tests ◽  
Suitable Alternative ◽  
Boundary Lubricant ◽  
Ultra High Molecular Weight

Ultra-high molecular weight polyethylene (UHMWPE) wear debris induced osteolysis has a major role in the late aseptic loosening and ultimate failure of total hip replacements (THR). Clinically relevant in vitro simulations of wear are essential to predict the osteolytic potential of bearing surfaces in artificial hip joints. Newborn calf or bovine serum has been accepted as a boundary lubricant for such in vitro tests, but its biological stability has been questioned. This study compared the wear factors, number of wear particles and levels of microbial contamination produced in bovine serum and a gelatin-based lubricant. The wear factors produced by the two lubricants were not significantly different, however the wear debris morphology produced was substantially different. The bovine serum became contaminated with micro-organisms within 28 h, whereas the protein-based lubricant remained uncontaminated. The results showed that bovine serum was not a stable boundary lubricant. They also showed that although the wear factors for the two solutions were not significantly different, the protein-based lubricant was not a suitable alternative to bovine serum because the wear debris produced was not clinically relevant.

scholarly journals Polyethylene Particles from a Hip Simulator Cause 45Ca Release from Cultured Bone

2001 ◽  
Vol 9 (2) ◽  
pp. 23-30 ◽  
Author(s):  
Kate Y Wang ◽  
J Geoffrey Horne ◽  
Peter A Devane ◽  
John H Miller
Keyword(s):  
Bone Resorption ◽  
Wear Debris ◽  
Polyethylene Wear ◽  
Dominant Factor ◽  
Dependent Manner ◽  
Calvarial Bone ◽  
Uhmwpe Wear ◽  
Hip Simulator ◽  
Short Term Exposure

Periprosthetic osteolysis is a dominant factor in the success or failure of total hip prostheses. Polyethylene wear debris has been implicated in the process of bone resorption and subsequent implant loosening. The present study is the first to examine the effect of ultra high molecular weight polyethylene (UHMWPE) wear debris produced by a hip simulator on calvarial bone resorption in vitro. 45Ca release was measured in cultured mouse calvarial bone samples. Although short-term exposure to UHMWPE particles (2 h) decreased 45Ca release, longer-term exposure for 1–2 days increased release in a dose-dependent manner. After one-day exposure to 7.5 × 106 particles per mL, 18% more 45Ca was released from cultured calvarial bone than from control samples. It was concluded that UHMWPE wear particles either directly or indirectly stimulated osteoclasts to activate bone resorption. Polyethylene wear debris contributes to the osteolytic process at the bone-implant interface.

scholarly journals Titanium-Nitride Coating of Orthopaedic Implants: A Review of the Literature

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ruud P. van Hove ◽  
Inger N. Sierevelt ◽  
Barend J. van Royen ◽  
Peter A. Nolte
Keyword(s):  
Titanium Nitride ◽  
Polyethylene Wear ◽  
Ultrahigh Molecular Weight Polyethylene ◽  
Coating Process ◽  
Orthopaedic Implant ◽  
Cohesive Failure ◽  
Orthopaedic Implants ◽  
Tin Coating ◽  
Titanium Nitride Coating ◽  
English Medical Literature

Surfaces of medical implants can be enhanced with the favorable properties of titanium-nitride (TiN). In a review of English medical literature, the effects of TiN-coating on orthopaedic implant material in preclinical studies were identified and the influence of these effects on the clinical outcome of TiN-coated orthopaedic implants was explored. The TiN-coating has a positive effect on the biocompatibility and tribological properties of implant surfaces; however, there are several reports of third body wear due to delamination, increased ultrahigh molecular weight polyethylene wear, and cohesive failure of the TiN-coating. This might be due to the coating process. The TiN-coating process should be optimized and standardized for titanium alloy articulating surfaces. The clinical benefit of TiN-coating of CoCrMo knee implant surfaces should be further investigated.

Can centrifugation affect the morphology of polyethylene wear debris?

Wear ◽  
2008 ◽  
Vol 265 (11-12) ◽  
pp. 1914-1917 ◽  
Author(s):  
E. Zolotarevova ◽  
Z. Fejfarkova ◽  
G. Entlicher ◽  
M. Lapcikova ◽  
M. Slouf ◽  
...  
Keyword(s):  
Wear Debris ◽  
Polyethylene Wear
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