Application of Hyperelastic Models in Mechanical Properties Prediction of Mouse Oocyte and Embryo Cells at Large Deformations

2016 ◽  
Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian ◽  
Ali Alizadeh ◽  
S. Tarighi
Keyword(s):  
Optimization Algorithm ◽  
Large Deformations ◽  
Poisson Ratio ◽  
Embryo Cell ◽  
Mouse Oocyte ◽  
Cell Manipulation ◽  
Levenberg Marquardt ◽  
Embryo Cells ◽  
Mechanical Properties Prediction

Biological cell studies have many applications in biology, cell manipulation and diagnosis of diseases such as cancer and malaria. In this study, inverse finite element method (IFEM) combined with Levenberg-Marquardt optimization algorithm has been used to extract and characterize material properties of mouse oocyte and embryo cells at large deformations. Then, the simulation results have been validated using data from experimental works. In this study, it is assumed cell material is hyperelastic, isotropic, homogenous and axisymmetric. For inverse analysis, FEM model of cell injection experiment which implemented in Abaqus software has been coupled with Levenberg-Marquardt optimization algorithm written in Matlab; based on this coupling the optimum hyperelastic coefficients which give the best match between experimental and simulated forces are extracted. Results show that among different hyperelastic material models, Ogden material is well suitable for characterization of mouse oocyte cell and Mooney-Rivlin or polynomial are suitable for characterization of mouse embryo cell. Moreover the evaluated Poisson ratio of the cell is obtained to be equal to 0.5, which indicates the structural material of mouse oocyte and embryo, are compressible.

Whole Cell Mechanical Property Characterization Based on Mechanical Properties of Its Cytoplasm and Bio Membrane

2012 ◽  
Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian
Keyword(s):  
Mechanical Properties ◽  
Finite Element ◽  
Optimization Algorithm ◽  
Mouse Oocyte ◽  
Model Parameters ◽  
Whole Cell ◽  
Levenberg Marquardt ◽  
Embryo Cells ◽  
Hyperelastic Model ◽  
Property Characterization

Analysis and investigation of the relation between different parts of biological cells such as biomembrane, cytoplasm and nucleus can help to better understand their behaviors and material properties. In this paper, first, the whole elastic properties of mouse oocyte and embryo cells have been computed by inverse finite element and Levenberg–Marquardt optimization algorithm and second, using the derived mechanical properties and the mechanical properties of its bio membrane from the literature, the mechanical properties of its cytoplasm has been characterized. It has been assumed that the cell behavior is as continues, isotropic, nonlinear and homogenous material for modeling. Matching the experimental forces with the forces from the finite element (FE) simulation by the Levenberg–Marquardt optimization algorithm, gives the nonlinear hyperelastic model parameters for the whole cell. Experimental data of mouse oocyte and embryo cells captured from the literatures.

Deformation Characterization of Mouse Oocyte Cell Using Inverse Finite Element and Levenberg–Marquardt Optimization Algorithm in Needle Injection Experiment

2012 ◽  
Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian
Keyword(s):  
Experimental Data ◽  
Mechanical Properties ◽  
Finite Element ◽  
Optimization Algorithm ◽  
Reaction Force ◽  
Mouse Oocyte ◽  
Material Behavior ◽  
Model Parameters ◽  
Levenberg Marquardt ◽  
Hyperelastic Model

In order to better understand the mechanical properties of biological cells, characterization and investigation of their material behavior is necessary. In this paper hyperelastic Neo-Hookean material is used to characterize the mechanical properties of mouse oocyte cell. It has been assumed that the cell behavior is continues, isotropic, nonlinear and homogenous material. Then, by matching the experimental data with finite element (FE) simulation result and using the Levenberg–Marquardt optimization algorithm, the nonlinear hyperelastic model parameters have been extracted. Experimental data of mouse oocyte captured from literatures. Advantage of the developed model is that it can be used to calculate accurate reaction force on surgical instrument or it can be used to compute deformation or force in virtual reality based medical simulations.

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

Transformation of hamster embryo cells by chymotrypsin-treated and untreated polyoma virus: characterization of transformants

1985 ◽  
Vol 31 (4) ◽  
pp. 356-360
Author(s):  
Vera Chlumecky ◽  
Donald C. Stranks ◽  
John S. Colter
Keyword(s):  
Common Species ◽  
Soft Agar ◽  
T Antigen ◽  
Polyoma Virus ◽  
Large T Antigen ◽  
T Antigens ◽  
Hamster Embryo Cells ◽  
Embryo Cells

The ability of chymotrypsin-treated (chymo+) and untreated (chymo−) polyoma virus to transform cultured hamster embryo fibroblasts was examined. The data show that exposure to this protease reduces the ability of the virus to transform non-permissive cells to essentially the same extent as it reduces its ability to replicate in permissive cells. Twenty-five lines of transformed cells were established from colonies growing in soft agar, and after 20 in vitro passages, cells of all lines were characterized with respect to their ability to form colonies in soft agar and their tumorigenicity in hamsters. While the studies showed that there are striking differences among the lines with respect to colony-forming ability, and real, though less striking differences in tumorigenicity, they failed to reveal any obvious differences between the groups of cell lines transformed by chymo− and chymo+ polyoma virus. Of 13 lines examined, all were found to express both middle and small polyoma T antigens, none express significant levels of large T antigen, and 11 express some form of what is probably a truncated large T antigen, the most common species having a molecular weight of 67 000.

scholarly journals Characterization of the Mercapturic Acid Pathway, an Important Phase II Biotransformation Route, in a Zebrafish Embryo Cell Line

2020 ◽  
Vol 33 (11) ◽  
pp. 2863-2871
Author(s):  
Alena Tierbach ◽  
Ksenia J. Groh ◽  
René Schoenenberger ◽  
Kristin Schirmer ◽  
Marc J.-F. Suter
Keyword(s):  
Cell Line ◽  
Phase Ii ◽  
Zebrafish Embryo ◽  
Embryo Cell ◽  
Mercapturic Acid ◽  
Mercapturic Acid Pathway ◽  
Acid Pathway

Early seed development in the annual and perennial Secale taxa

1985 ◽  
Vol 27 (2) ◽  
pp. 134-142 ◽  
Author(s):  
J. P. Gustafson ◽  
A. J. Lukaszewski
Keyword(s):  
Seed Development ◽  
Dna Content ◽  
Endosperm Development ◽  
Early Embryo ◽  
Embryo Cell ◽  
Developmental Patterns ◽  
Positive Correlation ◽  
Embryo Cells ◽  
Telomeric Heterochromatin ◽  
Early Seed Development

The early embryo and endosperm development patterns of five annual taxa and three perennial taxa of the genus Secale were analyzed. The results showed that there was considerable variation in the speed of early embryo and endosperm development within the genus Secale, and that the developmental patterns of the annual and perennial taxa overlapped. Comparisons indicated that DNA content per se did not have any influence on the speed of early embryo development or aberrant endosperm nucleus production in either the annual or perennial taxa. However, comparisons between the percent telomeric heterochromatin and the number of embryo cells produced showed a significant positive correlation in the annual taxa, and a nonsignificant correlation in the perennial taxa. There was a positive correlation between the number of aberrant endosperm nuclei and percent telomeric heterochromatin in the annual taxa, while the perennial taxa showed a nonsignificant but negative correlation. The results suggest that percent telomeric heterochromatin has a different effect on early seed development in the annual taxa than in the perennial taxa.Key words: Secale, heterochromatin, DNA content, embryo cell cycle.

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