Whole Cell Mechanical Property Characterization Based on Mechanical Properties of Its Cytoplasm and Bio Membrane

Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian

Analysis and investigation of the relation between different parts of biological cells such as biomembrane, cytoplasm and nucleus can help to better understand their behaviors and material properties. In this paper, first, the whole elastic properties of mouse oocyte and embryo cells have been computed by inverse finite element and Levenberg–Marquardt optimization algorithm and second, using the derived mechanical properties and the mechanical properties of its bio membrane from the literature, the mechanical properties of its cytoplasm has been characterized. It has been assumed that the cell behavior is as continues, isotropic, nonlinear and homogenous material for modeling. Matching the experimental forces with the forces from the finite element (FE) simulation by the Levenberg–Marquardt optimization algorithm, gives the nonlinear hyperelastic model parameters for the whole cell. Experimental data of mouse oocyte and embryo cells captured from the literatures.

Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian

In order to better understand the mechanical properties of biological cells, characterization and investigation of their material behavior is necessary. In this paper hyperelastic Neo-Hookean material is used to characterize the mechanical properties of mouse oocyte cell. It has been assumed that the cell behavior is continues, isotropic, nonlinear and homogenous material. Then, by matching the experimental data with finite element (FE) simulation result and using the Levenberg–Marquardt optimization algorithm, the nonlinear hyperelastic model parameters have been extracted. Experimental data of mouse oocyte captured from literatures. Advantage of the developed model is that it can be used to calculate accurate reaction force on surgical instrument or it can be used to compute deformation or force in virtual reality based medical simulations.


Author(s):  
Ali A. Abbasi ◽  
M. T. Ahmadian ◽  
Ali Alizadeh ◽  
S. Tarighi

Biological cell studies have many applications in biology, cell manipulation and diagnosis of diseases such as cancer and malaria. In this study, inverse finite element method (IFEM) combined with Levenberg-Marquardt optimization algorithm has been used to extract and characterize material properties of mouse oocyte and embryo cells at large deformations. Then, the simulation results have been validated using data from experimental works. In this study, it is assumed cell material is hyperelastic, isotropic, homogenous and axisymmetric. For inverse analysis, FEM model of cell injection experiment which implemented in Abaqus software has been coupled with Levenberg-Marquardt optimization algorithm written in Matlab; based on this coupling the optimum hyperelastic coefficients which give the best match between experimental and simulated forces are extracted. Results show that among different hyperelastic material models, Ogden material is well suitable for characterization of mouse oocyte cell and Mooney-Rivlin or polynomial are suitable for characterization of mouse embryo cell. Moreover the evaluated Poisson ratio of the cell is obtained to be equal to 0.5, which indicates the structural material of mouse oocyte and embryo, are compressible.


SPE Journal ◽  
2006 ◽  
Vol 11 (01) ◽  
pp. 5-17 ◽  
Author(s):  
Guohua Gao ◽  
Albert C. Reynolds

Summary For large scale history matching problems, where it is not feasible to compute individual sensitivity coefficients, the limited memory Broyden-Fletcher-Goldfarb-Shanno (LBFGS) is an efficient optimization algorithm, (Zhang and Reynolds, 2002; Zhang, 2002). However, computational experiments reveal that application of the original implementation of LBFGS may encounter the following problems:converge to a model which gives an unacceptable match of production data;generate a bad search direction that either leads to false convergence or a restart with the steepest descent direction which radically reduces the convergence rate;exhibit overshooting and undershooting, i.e., converge to a vector of model parameters which contains some abnormally high or low values of model parameters which are physically unreasonable. Overshooting and undershooting can occur even though all history matching problems are formulated in a Bayesian framework with a prior model providing regularization. We show that the rate of convergence and the robustness of the algorithm can be significantly improved by:a more robust line search algorithm motivated by the theoretical result that the Wolfe conditions should be satisfied;an application of a data damping procedure at early iterations orenforcing constraints on the model parameters. Computational experiments also indicate thata simple rescaling of model parameters prior to application of the optimization algorithm can improve the convergence properties of the algorithm although the scaling procedure used can not be theoretically validated. Introduction Minimization of a smooth objective function is customarily done using a gradient based optimization algorithm such as the Gauss- Newton (GN) method or Levenberg-Marquardt (LM) algorithm. The standard implementations of these algorithms (Tan and Kalogerakis, 1991; Wu et al., 1999; Li et al., 2003), however, require the computation of all sensitivity coefficients in order to formulate the Hessian matrix. We are interested in history matching problems where the number of data to be matched ranges from a few hundred to several thousand and the number of reservoir variables or model parameters to be estimated or simulated ranges from a few hundred to a hundred thousand or more. For the larger problems in this range, the computer resources required to compute all sensitivity coefficients would prohibit the use of the standard Gauss- Newton and Levenberg-Marquardt algorithms. Even for the smallest problems in this range, computation of all sensitivity coefficients may not be feasible as the resulting GN and LM algorithms may require the equivalent of several hundred simulation runs. The relative computational efficiency of GN, LM, nonlinear conjugate gradient and quasi-Newton methods have been discussed in some detail by Zhang and Reynolds (2002) and Zhang (2002).


Author(s):  
Mohammad Motalab ◽  
Munshi Basit ◽  
Jeffrey C. Suhling ◽  
Pradeep Lall

Traditional finite element based predictions for solder joint reliability during thermal cycling accelerated life testing are based on solder constitutive equations (e.g. Anand viscoplastic model) and failure models (e.g. energy dissipation per cycle model) that do not evolve with material aging. Thus, there will be significant errors in the calculations with lead free SAC alloys that illustrate dramatic aging phenomena. In this study, we have developed a revised set of Anand viscoplastic stress-strain relations for solder that include material parameters that evolve with the thermal history of the solder material. The effects of aging on the nine Anand model parameters have been examined by performing stress-strain tests on SAC305 samples that were aged for various durations (0–6 months) at temperature of 100 C. The stress-strain data were measured at three strain rates (.001, .0001, and .00001 1/sec) and five temperatures (25, 50, 75, 100, and 125 C). The mechanical tests have been performed using both water quenched (WQ) and reflowed (RF) samples (two unique specimen microstructures). In the case of the water quenched samples, there is rapid microstructural transitioning during the brief time that occurs between placing molten solder into the glass tubes and immersing the tubes in water bath. On the other hand, the reflowed samples are first cooled by water quenching, and then sent through a reflow oven to re-melt the solder in the tubes and subject them to a desired temperature profile matching that used in PCB assembly. As expected, the observed mechanical properties of water quenched samples were better (higher in magnitude) than the corresponding mechanical properties of the reflowed samples. Although the differences in elastic modulus between the water quenched and reflowed samples are small, significant differences are present for the yield and ultimate tensile stresses (for each aging condition). For both the water quenched and reflowed specimens, significant degradation of the mechanical properties has been observed with aging. Using the measured stress-strain and creep data, mathematical expressions have been developed for the evolution of the Anand model parameter with aging time. Our results show that 2 of the 9 constants remain essentially constant during aging, while the other 7 show large changes (30–70%) with up to 6 months of aging. The revised Anand constitutive equations for solder with aging effects have also been incorporated into commercial finite element codes (ANSYS and ABAQUS).


Author(s):  
Larry D. Carbary ◽  
Jon H. Kimberlain ◽  
John C. Oliva

Hyperelastic material model parameters have been developed to capture the behavior of silicone based construction sealants. Modern commercially available finite element analysis software makes it quite accessible to develop hyperelastic material models, automating the process of curve-fitting experimental lab data to specific hyperelastic formulations. However, the process of selecting a particular hyperelastic model from those supported is not straightforward. Here, a series of lab experiments are employed to guide the selection of the hyperelastic model that best describes various structural silicone glazings. A total of 10 different sealants are characterized with discussion of variations among the models. Comparisons of the best performing hyperelastic models for the different sealants are presented. Finally, an application is described in which these hyperelastic models have begun to be implemented in practice.


2020 ◽  
Vol 30 (2) ◽  
pp. 1-14
Author(s):  
Klaudia Śliwa-Wieczorek ◽  
Bogusław Zając ◽  
Tomasz Kozik

AbstractThe article presents testing of the mechanical properties of SIKA® polymer adhesives of the type PBM, PMM, PM, and PSM in the aspect of an attempt to determine the parameters of the Mooney-Rivlin hyperelastic model. The article contains a literature review on developed models for hyperelastic materials as well as a description of the author’s own results obtained in monaxial tensile and monaxial compression tests conducted on oars and cylindrical samples, respectively. Furthermore, the results of modeling of Mooney-Rivlin hyperelastic model parameters are shown in relation to the value of average parameters for polymers after both a week and a month-and-a-half of ripening.


2020 ◽  
Vol 14 (3) ◽  
pp. 7141-7151 ◽  
Author(s):  
R. Omar ◽  
M. N. Abdul Rani ◽  
M. A. Yunus

Efficient and accurate finite element (FE) modelling of bolted joints is essential for increasing confidence in the investigation of structural vibrations. However, modelling of bolted joints for the investigation is often found to be very challenging. This paper proposes an appropriate FE representation of bolted joints for the prediction of the dynamic behaviour of a bolted joint structure. Two different FE models of the bolted joint structure with two different FE element connectors, which are CBEAM and CBUSH, representing the bolted joints are developed. Modal updating is used to correlate the two FE models with the experimental model. The dynamic behaviour of the two FE models is compared with experimental modal analysis to evaluate and determine the most appropriate FE model of the bolted joint structure. The comparison reveals that the CBUSH element connectors based FE model has a greater capability in representing the bolted joints with 86 percent accuracy and greater efficiency in updating the model parameters. The proposed modelling technique will be useful in the modelling of a complex structure with a large number of bolted joints.


Symmetry ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 39
Author(s):  
Łukasz Warguła ◽  
Dominik Wojtkowiak ◽  
Mateusz Kukla ◽  
Krzysztof Talaśka

This article presents the results of experimental research on the mechanical properties of pine wood (Pinus L. Sp. Pl. 1000. 1753). In the course of the research process, stress-strain curves were determined for cases of tensile, compression and shear of standardized shapes samples. The collected data set was used to determine several material constants such as: modulus of elasticity, shear modulus or yield point. The aim of the research was to determine the material properties necessary to develop the model used in the finite element analysis (FEM), which demonstrates the symmetrical nature of the stress distribution in the sample. This model will be used to analyze the process of grinding wood base materials in terms of the peak cutting force estimation and the tool geometry influence determination. The main purpose of the developed model will be to determine the maximum stress value necessary to estimate the destructive force for the tested wood sample. The tests were carried out for timber of around 8.74% and 19.9% moisture content (MC). Significant differences were found between the mechanical properties of wood depending on moisture content and the direction of the applied force depending on the arrangement of wood fibers. Unlike other studies in the literature, this one relates to all three stress states (tensile, compression and shear) in all significant directions (anatomical). To verify the usability of the determined mechanical parameters of wood, all three strength tests (tensile, compression and shear) were mapped in the FEM analysis. The accuracy of the model in determining the maximum destructive force of the material is equal to the average 8% (for tensile testing 14%, compression 2.5%, shear 6.5%), while the average coverage of the FEM characteristic with the results of the strength test in the field of elastic-plastic deformations with the adopted ±15% error overlap on average by about 77%. The analyses were performed in the ABAQUS/Standard 2020 program in the field of elastic-plastic deformations. Research with the use of numerical models after extension with a damage model will enable the design of energy-saving and durable grinding machines.


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