Investigation of RBC Remodeling With a Multiscale Model

2010 ◽  
Author(s):  
Qiang Zhu ◽  
Zhangli Peng ◽  
Robert J. Asaro
Keyword(s):  
Blood Cell ◽  
Lipid Bilayer ◽  
Structural Stability ◽  
Composite Membrane ◽  
Red Blood Cell ◽  
Normal State ◽  
Single Layer ◽  
Multiscale Model ◽  
The Other ◽  
Mechanical Loads

Erythrocyte (red blood cell, or RBC) possesses one of the simplest and best characterized molecular architectures among all cells. It contains cytosol enclosed inside a composite membrane consisting of a fluidic lipid bilayer reinforced by a single layer of protein skeleton pinned to it. In its normal state, this system demonstrates tremendous structural stability, manifested in its ability to sustain large dynamic deformations during circulation. On the other hand, it has been illustrated in experiments that triggered by mechanical loads structural remodeling may occur. A canonical example of this remodeling is vesiculation, referring to the partial separation of the lipid bilayer from the protein skeleton and the formation of vesicles that contain lipids only.

TRICONCAVE SOLUTION TO THE HELFRICH VARIATION PROBLEM FOR THE SHAPE OF LIPID BILAYER VESICLES IS FOUND BY "SURFACE EVOLVER"

2002 ◽  
Vol 16 (03) ◽  
pp. 511-517 ◽  
Author(s):  
YONG ZHANG ◽  
XIN ZHOU ◽  
JIANJUN ZHOU ◽  
ZHONG-CAN OU-YANG
Keyword(s):  
Blood Cell ◽  
Lipid Bilayer ◽  
Red Blood Cell ◽  
Physical Parameters ◽  
Spontaneous Curvature ◽  
Fresh Blood ◽  
Surface Evolver ◽  
Variation Problem ◽  
Lipid Bilayer Vesicles

We numerically show the existence of triconcave Red Blood Cell (RBC) according to Helfrich spontaneous curvature model. It suggests that the Helfrich spontaneous curvature model can well work in the case of non-axisymmetric vesicle. Some geometric and physical parameters are obtained to describe the triconcave RBC which has been observed in fresh blood under the circumstance of certain hemolytic anemias. Comparing with the normal RBC, we find that the differences between the interior and the exterior of the triconcave RBC are smaller than that of the normal RBC.

scholarly journals Molecular Modelling of Human Red Blood Cell Pyruvate Kinase: Structural Implications of a Novel G1091 to A Mutation Causing Severe Nonspherocytic Hemolytic Anemia

Blood ◽  
1997 ◽  
Vol 90 (12) ◽  
pp. 4987-4995 ◽  
Author(s):  
Wouter W. van Solinge ◽  
Rob J. Kraaijenhagen ◽  
Gert Rijksen ◽  
Richard van Wijk ◽  
Bjarne B. Stoffer ◽  
...  
Keyword(s):  
Blood Cell ◽  
Pyruvate Kinase ◽  
Hemolytic Anemia ◽  
Red Blood Cell ◽  
Molecular Modelling ◽  
Human Liver ◽  
The Other ◽  
Salt Bridges ◽  
Compound Heterozygotes ◽  
Allosteric Properties

Abstract We present a novel G1091 to A mutation in the human liver and red blood cell (RBC) pyruvate kinase (PK) gene causing severe hemolytic anemia. In two families, three children were severely PK-deficient compound heterozygotes exhibiting the G1091 to A mutation and a common G1529 to A mutation on the other allele. In one family, the mother, a G1091 to A heterozygote, later had a second baby with a new husband, also a G1091 to A carrier. The baby was homozygous for the G1091 to A mutation and died 6 weeks after birth from severe hemolysis. Both mutant alleles were expressed at the RNA level. The G1091 to A mutation results in the substitution of a conserved glycine by an aspartate in domain A of RBC PK, whereas the G1529 to A mutation leads to the substitution of a conserved arginine residue with glutamine in the C-domain. Molecular modelling of human RBC PK, based on the crystal structure of cat muscle PK, shows that both mutations are located outside the catalytic site at the interface of domains A and C. The mutations are likely to disrupt the critical conformation of the interface by introducing alternative salt bridges. In this way the Gly364 to Asp and Arg510 to Gln substitutions may cause PK deficiency by influencing the allosteric properties of the enzyme.

Studies in Red Blood Cell Preservation 1. Effect of the Other Formed Elements

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 85-89 ◽  
Author(s):  
T. J. Greenwalt ◽  
C. Zehner Sostok ◽  
U. J. Dumaswala
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Cell Preservation

Studies in Red Blood Cell Preservation - 1. Effect of the Other Formed Elements

Vox Sanguinis ◽  
1990 ◽  
Vol 58 (2) ◽  
pp. 85-89
Author(s):  
C. Zehner Sostok ◽  
U.J. Dumaswala ◽  
T.J. Greenwalt
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Cell Preservation

Predicting red blood cell response to mechanical loads

Scilight ◽  
2021 ◽  
Vol 2021 (47) ◽  
pp. 471101
Author(s):  
Anne Cockshott
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Response ◽  
Mechanical Loads

scholarly journals IV. On the structure of the red blood-corpuscle of oviparous vertebrata

1869 ◽  
Vol 17 ◽  
pp. 346-350
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
The Other ◽  
Red Cell ◽  
Blood Corpuscle ◽  
Fundamental Distinction ◽  
Long Time ◽  
History Of

The red blood-cell has been perhaps more frequently and fully examined than any other animal structure; certainly none has evoked such various and even contradictory opinions of its nature. But without attempting here any history of these, it may be shortly said that amongst the conclusions now, and for a long time past, generally accepted, a chief one is that a fundamental distinction exists between the red corpuscle of Mammalia and that of the other vertebrate classes—that the red cell of the oviparous vertebrata possesses a nucleus which is not to be found in the corpuscle of the other class. This great distinction between the classes has of late years been over and over again laid down in the strongest and most unqualified terms. But I venture to ask for a still further examination of this important subject.

Presentation patterns and prognosis of 109 isolated venous injuries in 99 patients

2019 ◽  
Vol 34 (10) ◽  
pp. 698-706 ◽  
Author(s):  
Stavros K Kakkos ◽  
Ioannis A Tsolakis ◽  
George Markopoulos ◽  
Ioannis Maroulis ◽  
Efstratios Koletsis ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Odds Ratio ◽  
The Other ◽  
Outcome Predictors ◽  
Iatrogenic Injuries ◽  
Body Retention ◽  
All Cause Mortality ◽  
External Causes ◽  
Related Mortality

Objectives To identify outcome predictors of isolated venous injuries (VIs). Methods Retrospective analysis of prospectively collected information. Results A total of 99 patients with 109 isolated VI were included. All-cause mortality was 18/99 (18%) and mortality related to the VI was 10/99 (10%). On multivariate analysis, independent predictors of all-cause mortality included age (odds ratio – OR – 1.06, p = 0.042), external cause – trauma and foreign body retention – of VI (OR 34.62, p = 0.002) and the number of red blood cell units transfused intraoperatively (OR 2.10, p < 0.001), while independent predictors of VI-related mortality included external cause of VI (OR 47.60, p = 0.001) and the number of red blood cell units transfused intraoperatively (OR 1.72, p = 0.003). Conclusions VIs due to external causes have a high mortality rate. On the other hand, VIs due to internal causes (iatrogenic injuries during a surgical procedure) are managed promptly and have a very low mortality related to the VI.

Constitutive Modeling of the Finite Deformation Behavior of Membranes Possessing a Triangulated Network Microstructure

2005 ◽  
Vol 73 (4) ◽  
pp. 536-543 ◽  
Author(s):  
M. Arslan ◽  
M. C. Boyce
Keyword(s):  
Blood Cell ◽  
Mechanical Behavior ◽  
Lipid Bilayer ◽  
Red Blood Cell ◽  
Finite Deformation ◽  
Constitutive Modeling ◽  
Strain Energy ◽  
Shear Loading ◽  
Stress Strain ◽  
Spectrin Network

The mechanical behavior of the membrane of the red blood cell is governed by two primary microstructural features: the lipid bilayer and the underlying spectrin network. The lipid bilayer is analogous to a two-dimensional fluid in that it resists changes to its surface area, yet poses little resistance to shear. A skeletal network of spectrin molecules is cross-linked to the lipid bilayer and provides the shear stiffness of the membrane. Here, a general continuum level constitutive model of the large stretch behavior of the red blood cell membrane that directly incorporates the microstructure of the spectrin network is developed. The triangulated structure of the spectrin network is used to identify a representative volume element (RVE) for the model. A strain energy density function is constructed using the RVE together with various representations of the underlying molecular chain force-extension behaviors where the chain extensions are kinematically determined by the macroscopic deformation gradient. Expressions for the nonlinear finite deformation stress-strain behavior of the membrane are obtained by proper differentiation of the strain energy function. The stress-strain behaviors of the membrane when subjected to tensile and simple shear loading in different directions are obtained, demonstrating the capabilities of the proposed microstructurally detailed constitutive modeling approach in capturing the small to large strain nonlinear, anisotropic mechanical behavior. The sources of nonlinearity and evolving anisotropy are delineated by simultaneous monitoring of the evolution in microstructure including chain extensions, forces and orientations as a function of macroscopic stretch. The model captures the effect of pretension on the mechanical response where pretension is found to increase the initial modulus and decrease the limiting extensibility of the networked membrane.

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