An Automated Histologic System for 3D Histomorphometry of the Mouse Knee

2011 ◽  
Author(s):  
Barbara J. Murienne ◽  
Michael J. Girard ◽  
Lise Loerup ◽  
Alexandra Boussommier-Calleja ◽  
Sandra J. Shefelbine ◽  
...  
Keyword(s):  
Joint Space ◽  
Bone Defects ◽  
Structural Features ◽  
Degenerative Joint Disease ◽  
Cartilage Volume ◽  
Joint Disease ◽  
Point Counting ◽  
Geometrical Properties ◽  
3 Dimensional ◽  
And Cartilage

Osteoarthritis (OA) is a degenerative joint disease that is a leading cause of adult pain and disability in Western countries1. Clinically, several structural features of the joint are important in diagnosis, prognosis and evaluation of treatment efficacy, e.g. cartilage volume, homogeneity and joint space narrowing2,3. In animal models of OA, structural features such as bone defects and cartilage changes are commonly investigated using histomorphometry, a technique that uses stereological point counting and manual tracing of regions of interest to extract 3-dimensional (3D) geometrical properties from 2D histology slides. This is time-consuming and subject to inter-observer variations, hence limiting precision 4,5. Therefore, a technique for rapidly imaging joint structures in 3D at high resolution, including articular cartilage and subchondral bone, is currently needed in pre-clinical OA research.

A retrospective study of canine hip dysplasia in 116 military working dogs. Part I: Angle measurements and orthopedic foundation for animals (OFA) grading

1996 ◽  
Vol 32 (5) ◽  
pp. 413-422 ◽  
Author(s):  
CM Banfield ◽  
JE Bartels ◽  
JA Hudson ◽  
JC Wright ◽  
JT Hathcock ◽  
...  
Keyword(s):  
Hip Dysplasia ◽  
Joint Space Width ◽  
Joint Space ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Working Dogs ◽  
Estimated Risk ◽  
Width Measurements ◽  
Military Working Dogs ◽  
Canine Hip Dysplasia

The progression of hip dysplasia was investigated in 116 military working dogs. Serial pelvic radiographs were graded for degree of dysplasia and degenerative joint disease (DJD). Norberg angles, angles of inclination, and joint space widths were measured. There was a significant correlation between the Norberg angle and the degree of dysplasia (p less than 0.0001). Angles of inclination and joint space width measurements did not demonstrate a correlation to canine hip dysplasia. Dysplastic dogs had a significant estimated risk for development of DJD compared to normal dogs (p less than 0.0001; odds ratio of 70.2). Dogs with normal hip conformation at 24 months of age or older did not develop moderate nor severe DJD.

scholarly journals Integrative multiomics analysis of Premolis semirufa caterpillar venom in the search for molecules leading to a joint disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Giselle Pidde ◽  
Milton Y. Nishiyama ◽  
Ursula Castro de Oliveira ◽  
Isadora M. Villas-Boas ◽  
Adriana F. Paes-Leme ◽  
...  
Keyword(s):  
Bone Structure ◽  
Meta Analysis ◽  
Joint Stiffness ◽  
Cartilage Degeneration ◽  
Joint Space ◽  
Joint Disease ◽  
Homologous Proteins ◽  
Pharmacological Targets ◽  
Human Proteins ◽  
And Cartilage

AbstractThe joint disease called pararamosis is an occupational disease caused by accidental contact with bristles of the caterpillar Premolis semirufa. The chronic inflammatory process narrows the joint space and causes alterations in bone structure and cartilage degeneration, leading to joint stiffness. Aiming to determine the bristle components that could be responsible for this peculiar envenomation, in this work we have examined the toxin composition of the caterpillar bristles extract and compared it with the differentially expressed genes (DEGs) in synovial biopsies of patients affected with rheumatoid arthritis (RA) and osteoarthritis (OA). Among the proteins identified, 129 presented an average of 63% homology with human proteins and shared important conserved domains. Among the human homologous proteins, we identified seven DEGs upregulated in synovial biopsies from RA or OA patients using meta-analysis. This approach allowed us to suggest possible toxins from the pararama bristles that could be responsible for starting the joint disease observed in pararamosis. Moreover, the study of pararamosis, in turn, may lead to the discovery of specific pharmacological targets related to the early stages of articular diseases.

scholarly journals A green-lipped mussel reduces pain behavior and chondrocyte inflammation and attenuated experimental osteoarthritis progression

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0259130
Author(s):  
JooYeon Jhun ◽  
Hyun Sik Na ◽  
Keun-Hyung Cho ◽  
Jiyoung Kim ◽  
Young-Mee Moon ◽  
...  
Keyword(s):  
Cartilage Damage ◽  
Cartilage Degeneration ◽  
Kinase Domain ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Omega 3 ◽  
Expression Levels ◽  
Oa Chondrocytes ◽  
And Cartilage

The green-lipped mussel (GLM) contains novel omega-3 polyunsaturated fatty acids, which exhibit anti-inflammatory and joint-protecting properties. Osteoarthritis (OA) is a degenerative joint disease characterized by a progressive loss of cartilage; oxidative stress plays a role in the pathogenesis of OA. The objectives of this study were to investigate the in vivo effects of the GLM on pain severity and cartilage degeneration using an experimental rat OA model, and to explore the mode of action of GLM. OA was induced in rats by intra-articular injection of monosodium iodoacetate (MIA) into the knee. Oral GLM was initiated on the day after 3dyas of MIA injection. Limb nociception was assessed by measuring the paw withdrawal latency and threshold. Samples were analyzed both macroscopically and histologically. Immunohistochemistry was used to investigate the expression of interleukin-1β (IL-1β), IL-6, nitrotyrosine, and inducible nitric oxide synthase (iNOS) in knee joints. Also, the GLM was applied to OA chondrocyte, and the expression on catabolic marker and necroptosis factor were evaluated by real-time polymerase chain reaction. Administration of the GLM improved pain levels by preventing cartilage damage and inflammation. GLM significantly attenuated the expression levels of mRNAs encoding matrix metalloproteinase-3 (MMP-3), MMP-13, and ADAMTS5 in IL-1β-stimulated human OA chondrocytes. GLM decreased the expression levels of the necroptosis mediators RIPK1, RIPK3, and the mixed lineage kinase domain-like protein (MLKL) in IL-1β-stimulated human OA chondrocytes. Thus, GLM reduced pain and cartilage degeneration in rats with experimentally induced OA. The chondroprotective properties of GLM included suppression of oxidative damage and inhibition of catabolic factors implicated in the pathogenesis of OA cartilage damage. We suggest that GLM may usefully treat human OA.

scholarly journals The Role of Extracellular Vesicles in the Pathogenesis, Diagnosis, and Treatment of Osteoarthritis

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4987
Author(s):  
Jianjing Lin ◽  
Li Wang ◽  
Jianhao Lin ◽  
Qiang Liu
Keyword(s):  
Extracellular Vesicles ◽  
Cartilage Regeneration ◽  
Degenerative Joint Disease ◽  
Diagnosis And Treatment ◽  
Joint Disease ◽  
Significant Progress ◽  
Cell Pretreatment ◽  
Cell Sources ◽  
And Cartilage

Osteoarthritis (OA) is a degenerative joint disease that affects the entire joint and has been a tremendous burden on the health care system worldwide. Although cell therapy has made significant progress in the treatment of OA and cartilage regeneration, there are still a series of problems. Recently, more and more evidence shows that extracellular vesicles (EVs) play an important role in the progression and treatment of OA. Here, we discuss that EVs from different cell sources not only participate in OA progression, but can also be used as effective tools for the diagnosis and treatment of OA. In addition, cell pretreatment strategies and EV tissue engineering play an increasingly prominent role in the field of OA treatment. This article will systematically review the latest developments in these areas. As stated above, it may provide new insights for improving OA and cartilage regeneration.

Arthritis of the Knee

2017 ◽  
Author(s):  
Vandana Sharma ◽  
Kevin Anuvat ◽  
Liju John ◽  
Matthew Davis
Keyword(s):  
Platelet Rich Plasma ◽  
Single Agent ◽  
Botulinum Toxin Type ◽  
Joint Space ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Sufficient Evidence ◽  
Osteoarthritis Of The Knee ◽  
Lifestyle Modifications ◽  
Antiinflammatory Drugs

Osteoarthritis (OA) of the knee is the most common form of degenerative joint disease, affecting 13.9% of adults age 25 years and older and 33.6% of those 65 years and older. Some predisposing risk factors include age, obesity, infection, repetitive use, occupation, and previous trauma. Mild OA usually presents as pain in the knee with difficulty bending or straightening the knee, swelling, weakness, instability, locking, and clicking or snapping of the knee. A diagnosis can be made clinically and confirmed by x-ray showing narrowing of the joint space in mild cases; however, bone changes, subchondral cysts, and formation of bony spurs can be seen in more advanced stages. Treatment of mild to moderate OA involves a multimodal approach that includes lifestyle modifications, physical therapy, and electrical stimulation with or without pharmacologic management. Interventional therapies such as intra-articular corticosteroid injections and viscosupplementation are usually reserved for severe symptoms. Pharmacologic modalities such as acetaminophen, nonsteroidal antiinflammatory drugs, weak opioids, and duloxetine can be used as a single agent or combination therapy. Novel approaches to the treatment of OA include nerve growth factor inhibitors, botulinum toxin type B injections, and intra-articular platelet-rich plasma but are not well supported due to a lack of sufficient evidence. Key words: arthritis, degenerative joint disease of the knee, diagnosis, etiology, guidelines, noninflammatory arthritis, osteoarthritis of the knee, treatment

Apportionment of Impairment from Meniscal Tears and Knee Arthritis

2013 ◽  
Vol 18 (5) ◽  
pp. 1-10 ◽  
Author(s):  
Charles N. Brooks ◽  
James B. Talmage
Keyword(s):  
Medial Meniscus ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Detailed Knowledge ◽  
Meniscal Tears ◽  
Knee Arthritis ◽  
Degenerative Arthritis ◽  
Study Results ◽  
Physical Findings ◽  
Causation Analysis

Abstract Meniscal tears and osteoarthritis (osteoarthrosis, degenerative arthritis, or degenerative joint disease) are two of the most common conditions involving the knee. This article includes definitions of apportionment and causes; presents a case report of initial and recurrent tears of the medial meniscus plus osteoarthritis (OA) in the medial compartment of the knee; and addresses questions regarding apportionment. The authors, experienced impairment raters who are knowledgeable regarding the AMA Guides to the Evaluation of Permanent Impairment (AMA Guides), show that, when instructions on impairment rating are incomplete, unclear, or inconsistent, interrater reliability diminishes (different physicians may derive different impairment estimates). Accurate apportionment of impairment is a demanding task that requires detailed knowledge of causation for the conditions in question; the mechanisms of injury or extent of exposures; prior and current symptoms, functional status, physical findings, and clinical study results; and use of the appropriate edition of the AMA Guides. Sometimes the available data are incomplete, requiring the rating physician to make assumptions. However, if those assumptions are reasonable and consistent with the medical literature and facts of the case, if the causation analysis is plausible, and if the examiner follows impairment rating instructions in the AMA Guides (or at least uses a rational and hence defensible method when instructions are suboptimal), the resulting apportionment should be credible.

3. Degenerative joint disease

2000 ◽  
Vol 81 (3B) ◽  
pp. s67-s72
Author(s):  
Victoria A. Brander ◽  
Darryl L. Kaelin ◽  
Terry H. Oh ◽  
Peter A.C. Lim
Keyword(s):  
Degenerative Joint Disease ◽  
Joint Disease

Osteotomy for Correction of Premature Growth Plate Closure in 24 Dogs

1994 ◽  
Vol 07 (03) ◽  
pp. 129-135 ◽  
Author(s):  
C.W. Miller ◽  
P.W. Morgan
Keyword(s):  
Corrective Osteotomy ◽  
Functional Results ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Angular Deformity ◽  
Dynamic Correction ◽  
Age At Surgery ◽  
Limb Deformities ◽  
The Mean ◽  
A Prospective Study

SummaryTwenty-four dogs (27 limbs) were evaluated after surgery for correction of forelimb angular limb deformities. Partial ulnar ostectomies or definitive corrective osteotomies were performed depending upon the age of the dog. According to owner assessment nine of fourteen limbs were considered functionally good, or excellent, after partial ulnar ostectomies. Younger dogs appeared to have better functional results after dynamic correction with the mean age at surgery of dogs with good to excellent results being 6.5 months contrasted to the mean age at surgery of dogs with fair to poor results being 9.75 months. Ten of fourteen limbs were considered functionally good or excellent after definitive corrective osteotomy. One dog had definitive osteotomy after partial ulnar ostectomy in order to further correct a residual angular deformity. However, 58% of the limbs with radiographic follow-up had signs of degenerative joint disease (DJD). There were not significant differences between neither degree of angulation remaining after surgery and the functional result nor the degree of angulation remaining after surgery and the development of DJD. A prospective study is warranted to more objectively assess the efficacy of surgical correction of angular limb deformities in dogs.Twenty-four dogs were evaluated after surgery for correction of forelimb angular limb deformities. The results are described.

Anticoagulant Properties of Three Mucopolysaccharides Used in Rheumatology

1983 ◽  
Vol 50 (03) ◽  
pp. 652-655 ◽  
Author(s):  
F Bauer ◽  
P Schulz ◽  
G Reber ◽  
C A Bouvier
Keyword(s):  
Factor Xa ◽  
Degenerative Joint Disease ◽  
Joint Disease ◽  
Thrombin Time ◽  
Coagulation Tests ◽  
Amplification System ◽  
Relative Potencies ◽  
In Vivo Administration

SummaryThree mucopolysaccharides (MPS) used in the treatment of degenerative joint disease were compared to heparin to establish their relative potencies on 3 coagulation tests, the aPTT, the antifactor X a activity and the dilute thrombin time. One of the compounds, Arteparon®, was one fourth as potent as heparin on the aPTT, but had little or no influence on the 2 other tests. Further in vitro studies suggested that Arteparon® acted at a higher level than factor Xa generation in the intrinsic amplification system and that its effect was independent of antithrombin III. In vivo administration of Arteparon® confirmed its anticoagulant properties, which raises the question of the clinical use of this MPS.

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