Fluorescence detection of protoporphyrin IX in living cells: a comparative study on single- and two-photon excitation

2008 ◽  
Vol 13 (2) ◽  
pp. 024014 ◽  
Author(s):  
Sijia Lu ◽  
Ji-Yao Chen ◽  
Yu Zhang ◽  
Jiong Ma ◽  
Pei-Nan Wang ◽  
...  
Keyword(s):  
Comparative Study ◽  
Fluorescence Detection ◽  
Protoporphyrin Ix ◽  
Living Cells ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals Two-photon excitation in living cells induced by low-power cw laser beams

1996 ◽  
Author(s):  
Karsten Koenig ◽  
Tatiana B. Krasieva ◽  
Yagang Liu ◽  
Michael W. Berns ◽  
Bruce J. Tromberg
Keyword(s):  
Low Power ◽  
Living Cells ◽  
Laser Beams ◽  
Two Photon ◽  
Cw Laser ◽  
Photon Excitation ◽  
Two Photon Excitation

scholarly journals A novel method for observing proteins in vivo using a small fluorescent label and multiphoton imaging

2005 ◽  
Vol 390 (3) ◽  
pp. 787-790 ◽  
Author(s):  
Stanley W. Botchway ◽  
Ignasi Barba ◽  
Randolf Jordan ◽  
Rebecca Harmston ◽  
Peter M. Haggie ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Fluorescence Emission ◽  
Yeast Cells ◽  
Fluorescent Label ◽  
Multiphoton Imaging ◽  
Two Photon ◽  
Photon Excitation ◽  
Novel Method ◽  
Two Photon Excitation

A novel method for the fluorescence detection of proteins in cells is described in the present study. Proteins are labelled by the selective biosynthetic incorporation of 5-hydroxytryptophan and the label is detected via selective two-photon excitation of the hydroxyindole and detection of its fluorescence emission at 340 nm. The method is demonstrated in this paper with images of a labelled protein in yeast cells.

Determination of acceptor-to-donor cross section ratio for two-photon excitation in living cells

2016 ◽  
Author(s):  
Zexian Hou ◽  
Yuhua Wang ◽  
Liqin Zheng ◽  
Tongsheng Chen ◽  
Hongqin Yang ◽  
...  
Keyword(s):  
Cross Section ◽  
Living Cells ◽  
Cross Section Ratio ◽  
Two Photon ◽  
Section Ratio ◽  
Photon Excitation ◽  
Two Photon Excitation

Real-time monitoring of intracellular nitric oxide using a long-wavelength-emitting probe via one-photon or two-photon excitation

2019 ◽  
Vol 7 (11) ◽  
pp. 3246-3252 ◽  
Author(s):  
Changquan Tang ◽  
Mingxue Wang ◽  
Xiaoying Shang ◽  
Xueyuan Chen ◽  
Deguang Huang ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Real Time ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Long Wavelength ◽  
Two Photon ◽  
Turn On ◽  
Photon Excitation ◽  
Endogenous Nitric Oxide ◽  
Two Photon Excitation

A long-wavelength-emitting fluorescent turn-on probe is developed for monitoring exogenous or endogenous nitric oxide in living cells.

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