Self-checking pantograph-catenary arcing fluorescent detection system based on fiber sensing

2017 ◽  
Author(s):  
Yuming Dong ◽  
Yizhou Zhang ◽  
Liang Zhang ◽  
Peng Liu ◽  
Liangpei Chen
Keyword(s):  
Detection System ◽  
Fluorescent Detection ◽  
Fiber Sensing

Abstract 4336: Interrogating the Human Myocardial Interstitium during Myocardial Arrest and Reperfusion: Dynamic Changes in Cytokines and Protease Activity

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Robert E Stroud ◽  
Christine N Koval ◽  
Isabelle Gengler ◽  
Anne M Deschamps ◽  
John S Ikonomidis ◽  
...  
Keyword(s):  
Detection System ◽  
Ischemia Reperfusion ◽  
Dynamic Assessment ◽  
High Sensitivity ◽  
Fluorescent Detection ◽  
Suspension Array ◽  
Cytokine Analysis ◽  
And Function ◽  
Inflammatory Molecules ◽  
Pulmonary Bypass

Background. Cytokines, such as the interleukins (IL1β, IL2, IL6) and tumor necrosis factor (TNF) can modulate myocardial structure and function with ischemia/reperfusion (I/R) but dynamic assessment of these biological molecules within the human myocardial interstitium with I/R has not been performed, and the inter-relationship to matrix metalloproteinases activity (MMPact) remains unexplored. Accordingly, a fluorogenic microdialysis method was used to simultaneously measure myocardial interstitial cytokine levels and MMPact in patients during and following I/R. Methods . MMPact was measured in patients (n=13) undergoing cardio-pulmonary bypass (CPB) at baseline, during myocardial arrest and CPB (on-CPB), and immediately following reperfusion and separation from CPB (post-CPB) by a validated in-line microdialysis fluorescent detection system. Myocardial interstitial fluid was subjected to cytokine analysis by high sensitivity multiplex suspension array. Results . Interstitial MMPact increased by over 30% post-CPB and was accompanied by a specific change in cytokine profiles (Figure ). The classical pro-inflammatory molecules such as TNF and IL6 were either not detectable or unchanged, whereas IL1β and IL2 which can be proinflammatory, were increased. Conclusions. These unique results demonstrated that a dynamic cytokine signature occurs within the human myocardial interstitium following I/R and is temporally related to heightened MMP activity. Direct interrogation of the human myocardial interstitium may provide a unique insight into critical signaling pathways which may evoke adverse structural and functional events following I/R.

scholarly journals Development of a New Laparoscopic Detection System for Gastric Cancer Using Near-Infrared Light-Emitting Clips with Glass Phosphor

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 81
Author(s):  
Shunko Inada ◽  
Hayao Nakanishi ◽  
Masahiro Oda ◽  
Kensaku Mori ◽  
Akihiro Ito ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Laparoscopic Surgery ◽  
Near Infrared ◽  
Detection System ◽  
Fluorescent Detection ◽  
Infrared Light ◽  
Excitation Light ◽  
Light Emitting ◽  
Nir Light ◽  
Glass Phosphor

Laparoscopic surgery is now a standard treatment for gastric cancer. Currently, the location of the gastric cancer is identified during laparoscopic surgery via the preoperative endoscopic injection of charcoal ink around the primary tumor; however, the wide spread of injected charcoal ink can make it difficult to accurately visualize the specific site of the tumor. To precisely identify the locations of gastric tumors, we developed a fluorescent detection system comprising clips with glass phosphor (Yb3+, Nd3+ doped to Bi2O3-B2O3-based glasses, size: 2 mm × 1 mm × 3 mm) fixed in the stomach and a laparoscopic fluorescent detection system for clip-derived near-infrared (NIR) light (976 nm). We conducted two ex vivo experiments to evaluate the performance of this fluorescent detection system in an extirpated pig stomach and a freshly resected human stomach and were able to successfully detect NIR fluorescence emitted from the clip in the stomach through the stomach wall by the irradiation of excitation light (λ: 808 nm). These results suggest that the proposed combined NIR light-emitting clip and laparoscopic fluorescent detection system could be very useful in clinical practice for accurately identifying the location of a primary gastric tumor during laparoscopic surgery.

Integrated semiconductor fluorescent detection system for biochip and biomedical applications

2002 ◽  
Author(s):  
Evan P. Thrush ◽  
Ofer Levi ◽  
Ke Wang ◽  
Mark A. Wistey ◽  
James S. Harris, Jr. ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Detection System ◽  
Fluorescent Detection

DNA Hybridization Enhancement in Microarrays Using AC-Electrothermal Flow

2008 ◽  
Author(s):  
F. Bottausci ◽  
T. Neumann ◽  
M. A. Mader ◽  
I. Mezic ◽  
L. Jaeger ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Reaction Rate ◽  
Detection System ◽  
Experimental Results ◽  
Fluorescent Detection ◽  
Chip Surface ◽  
Diffusion Limited ◽  
On Chip ◽  
Electrothermal Flow ◽  
Hybridization Reaction

We present numerical and experimental results on an innovative method to enhance the hybridization process in the field of microarray based analytics. Hybridization reaction based on biochip approaches take several hours due to the fact that these reactions are diffusion limited. To monitor the binding of fluorescent labeled analyte molecules to the probe molecules on the chip surface we use a home made fluorescent detection system that allows continues monitoring of the reaction on chip. Mixing is the key to overcome the diffusion barrier and increase reaction rate. We present a novel mixing device that increases reaction time The AC-electrokinetic signal produces an electrothermal flow that enhances the DNA binding. We show an optimization of the AC-signal and the electrodes design based on numerical simulations to obtain highest efficiency in mixing of analyte solutions and we report experimental results on dephosphorylation experiment.

scholarly journals A new fluorescent detection system for identifying variant hemoglobins after gel electrophoresis using immunobinding with monoclonal antibodies.

1986 ◽  
Vol 34 (5) ◽  
pp. 585-591 ◽  
Author(s):  
R E Smith
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Gel Electrophoresis ◽  
Detection System ◽  
Great Sensitivity ◽  
Fluorescent Detection ◽  
Fluorogenic Substrate ◽  
Allelic Variants ◽  
Resolution System ◽  
Entire Procedure

This paper describes a low-resolution system for identifying variant hemoglobins with great sensitivity and specificity. After electrophoresis of the hemoglobin sample in a gel, fixation is used to entrap the hemoglobin. The gel is dried, incubated with a monoclonal antibody against the desired hemoglobin, then incubated with a second antibody against the first antibody which is conjugated with the enzyme beta-d-galactosidase. An enzyme overlay membrane containing a fluorogenic substrate is then placed on the gel surface, incubated, and removed, yielding an immunofluorescent print. The entire procedure takes only two hours, and by virtue of fluorescent detection gives sharper band resolution and greater sensitivity than conventional dye methods. The system clearly distinguishes SS sickle-cell hemoglobin from heterozygous and "S-like" hemoglobins. The technique therefore holds promise as a powerful probe for allelic variants.

scholarly journals Detection of Mycoplasma genitalium in female cervical samples by Multitarget Real-Time PCR

2007 ◽  
Vol 7 (2) ◽  
pp. 109-112
Author(s):  
Sabina Mahmutović-Vranić ◽  
Edina Bešlagić ◽  
Justin Hardick ◽  
Maida Šlaković ◽  
Charlotte A. Gaydos
Keyword(s):  
Real Time ◽  
Large Scale ◽  
Detection System ◽  
Mycoplasma Genitalium ◽  
Positive Control ◽  
Positive Sample ◽  
Fluorescent Detection ◽  
Cross Point ◽  
Sequence Detection ◽  
Urogenital Infections

Mycoplasma genitalum (MG) is associated with variety of urogenital infections such as non-gonococcal urethritis (NGU), endometritis and cervicitis. The objective of this study was to demonstrate and evaluate a research polymerase chain reaction (PCR) assay, for the detection of MG in cervical samples of a tested population of women attending gynecology clinics in Bosnia and Herzegovina. The Multitarget Real-Time (MTRT) PCR, utilizing the ABI 7900HT, the sequence detection system, was performed for the detection of MG. Cervical samples (N=97) from females were divided into three types of patient groups: Group 1: patients who had known abnormal clinical cytology reports (N=34); Group 2: patients who reported a history of genitourinary infections (N=22); and Group 3: patients not in either groups 1 or 2 (N=41). Overall, 14,43% (14/97) of those tested were positive for MG. A positive sample was defined as having a cycle threshold cross point (Ct) < 40,0 with a fluorescent detection comparable to the low positive control utilized during the run. This study validated the use of MTRT PCR as a reliable method for the detection of MG in clinical specimens and should facilitate large-scale screening for this organism.

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