Preliminary in-vivo imaging evaluation of patient-specific scatter-corrected digital chest tomosynthesis

Intraportal injection is regarded as the current standard procedure of hepatocyte transplantation (HTx). In islet transplantation, which shares many aspects with HTx, recent studies have clarified that instant blood-mediated inflammatory reaction (IBMIR), characterized by strong innate immune responses, can cause poor engraftment, so other transplant sites to avoid such a reaction have been established. Although IBMIR was reported to occur in HTx, few reports have evaluated alternative transplant sites for HTx. In this study, we sought to determine the optimum transplant site for HTx. Rat hepatocytes (1.0 × 107) were transplanted at the 9 transplant sites (intraportal (IPO), intrasplenic (IS), liver parenchyma, subcutaneous, intraperitoneal, renal subcapsular, muscle, inguinal subcutaneous white adipose tissue, and omentum) of analbuminemic rats. The serum albumin levels, immunohistochemical staining (albumin, TUNEL, and BrdU), and in vivo imaging of the grafts were evaluated. The serum albumin levels of the IPO group were significantly higher than those of the other groups ( p < .0001). The BrdU-positive hepatocyte ratio of liver in the IS group (0.9% ± 0.2%) was comparable to that of the IPO group (0.9% ± 0.3%) and tended to be higher than that of the spleen in the IS group (0.5% ± 0.1%, p = .16). Considering the in vivo imaging evaluation and the influence of splenectomy, the graft function in the IS group may be almost entirely achieved by hepatocytes that have migrated to the liver. The present study clearly showed that the intraportal injection procedure is more efficient than other procedures for performing HTx

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Carotid Bifurcation With Tandem Stenosis—A Patient-Specific Case Study Combined in vivo Imaging, in vitro Histology and in silico Simulation

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2019.00349 ◽

2019 ◽

Vol 7 ◽

Cited By ~ 2

Author(s):

Jiaqiu Wang ◽

Phani Kumari Paritala ◽

Jessica Benitez Mendieta ◽

Yuantong Gu ◽

Owen Christopher Raffel ◽

...

Keyword(s):

In Vivo Imaging ◽

In Silico ◽

Carotid Bifurcation ◽

Patient Specific ◽

Tandem Stenosis ◽

In Silico Simulation

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Analysis and Algorithmic Generation of Hepatic Vascular Systems

International Journal of Hepatology ◽

10.1155/2012/357687 ◽

2012 ◽

Vol 2012 ◽

pp. 1-17 ◽

Cited By ~ 23

Author(s):

Lars Ole Schwen ◽

Tobias Preusser

Keyword(s):

In Vivo Imaging ◽

Geometric Model ◽

Statistical Testing ◽

Ct Scans ◽

Patient Specific ◽

Vascular Tree ◽

Data Set ◽

Tree Data ◽

Vascular Trees

A proper geometric model of the vascular systems in the liver is crucial for modeling blood flow, the connection between the organ and the rest of the organism. In vivo imaging does not provide sufficient details, so an algorithmic concept for extending measured vascular tree data is needed such that geometrically realistic structures can be generated. We develop a quantification of similarity in terms of different geometric features. This involves topological Strahler ordering of the vascular trees, statistical testing, and averaging. Invariant features are identified in human clinical in vivo CT scans. Results of the existing “Constrained Constructive Optimization” algorithm are compared to real vascular tree data. To improve bifurcation angles in the algorithmic results, we implement a postprocessing step calibrated to the measured features. This framework is finally applied to generate realistic additional details in a patient-specific hepatic vascular tree data set.

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Formulation and in vivo imaging evaluation of colonic targeting tablets prepared by a simple dry powder coating technique

Journal of Pharmaceutical Investigation ◽

10.1007/s40005-019-00463-x ◽

2019 ◽

Vol 50 (4) ◽

pp. 383-398 ◽

Cited By ~ 3

Author(s):

Nguyen-Thach Tung ◽

Canh-Hung Nguyen ◽

Van-Duong Nguyen ◽

Thi-Hong-Thuy Nguyen ◽

Van-Lam Nguyen ◽

...

Keyword(s):

In Vivo Imaging ◽

Powder Coating ◽

Dry Powder ◽

Imaging Evaluation ◽

Coating Technique ◽

Dry Powder Coating

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Before In Vivo Imaging: Evaluation of Fluorescent Probes Using Fluorescence Microscopy, Multiplate Reader, and Cytotoxicity Assays

In Vivo Fluorescence Imaging - Methods in Molecular Biology ◽

10.1007/978-1-4939-3721-9_1 ◽

2016 ◽

pp. 1-13

Author(s):

Shaojuan Zhang

Keyword(s):

Fluorescence Microscopy ◽

Fluorescent Probes ◽

In Vivo Imaging ◽

Imaging Evaluation ◽

Cytotoxicity Assays

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Fluorescent proteins for in vivo imaging, where's the biliverdin?

Biochemical Society Transactions ◽

10.1042/bst20200444 ◽

2020 ◽

Vol 48 (6) ◽

pp. 2657-2667

Author(s):

Felipe Montecinos-Franjola ◽

John Y. Lin ◽

Erik A. Rodriguez

Keyword(s):

Hydrogen Peroxide ◽

In Vivo Imaging ◽

Near Infrared ◽

Fluorescent Protein ◽

Fluorescent Proteins ◽

Fluorescent Imaging ◽

Infrared Light ◽

Red Fluorescent Protein ◽

Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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