scholarly journals Mobilizable IncQ-Related Plasmid Carrying a New Quinolone Resistance Gene, qnrS2, Isolated from the Bacterial Community of a Wastewater Treatment Plant

2006 ◽  
Vol 50 (9) ◽  
pp. 3075-3080 ◽  
Author(s):  
Gabriele Bönemann ◽  
Michael Stiens ◽  
Alfred Pühler ◽  
Andreas Schlüter
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Activated Sludge ◽  
Resistance Gene ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
The United States ◽  
Replication Initiation ◽  
Quinolone Resistance ◽  
Selection Strategy

ABSTRACT Plasmid-encoded quinolone resistance was previously reported for different bacteria isolated from patients not only in the United States and Asia but also in Europe. Here we describe the isolation, by applying a new selection strategy, of the quinolone resistance plasmid pGNB2 from an activated sludge bacterial community of a wastewater treatment plant in Germany. The hypersensitive Escherichia coli strain KAM3 carrying a mutation in the multidrug efflux system genes acrAB was transformed with total plasmid DNA preparations isolated from activated sludge bacteria and subsequently selected on medium containing the fluoroquinolone norfloxacin. This approach resulted in the isolation of plasmid pGNB2 conferring decreased susceptibility to nalidixic acid and to different fluoroquinolones. Analysis of the pGNB2 nucleotide sequence revealed that it is 8,469 bp in size and has a G+C content of 58.2%. The plasmid backbone is composed of a replication initiation module (repA-repC) belonging to the IncQ-family and a two-component mobilization module that confers horizontal mobility to the plasmid. In addition, plasmid pGNB2 carries an accessory module consisting of a transposon Tn1721 remnant and the quinolone resistance gene, qnrS2, that is 92% identical to the qnrS gene located on plasmid pAH0376 from Shigella flexneri 2b. QnrS2 belongs to the pentapeptide repeat protein family and is predicted to protect DNA-gyrase activity against quinolones. This is not only the first report on a completely sequenced plasmid mediating quinolone resistance isolated from an environmental sample but also on the first qnrS-like gene detected in Europe.

scholarly journals IncP-1β Plasmid pGNB1 Isolated from a Bacterial Community from a Wastewater Treatment Plant Mediates Decolorization of Triphenylmethane Dyes

2007 ◽  
Vol 73 (20) ◽  
pp. 6345-6350 ◽  
Author(s):  
Andreas Schlüter ◽  
Irene Krahn ◽  
Florian Kollin ◽  
Gabriele Bönemann ◽  
Michael Stiens ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Community ◽  
Crystal Violet ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Replication Initiation ◽  
Host Bacterium ◽  
Triphenylmethane Dyes ◽  
Reductase Gene ◽  
Triphenylmethane Reductase

ABSTRACT Plasmid pGNB1 was isolated from bacteria residing in the activated sludge compartment of a wastewater treatment plant by using a transformation-based approach. This 60-kb plasmid confers resistance to the triphenylmethane dye crystal violet and enables its host bacterium to decolorize crystal violet. Partial sequencing of pGNB1 revealed that its backbone is very similar to that of previously sequenced IncP-1β plasmids. The two accessory regions of the plasmid, one located downstream of the replication initiation gene trfA and the other located between the conjugative transfer modules Tra and Trb, were completely sequenced. Accessory region L1 contains a transposon related to Tn5501 and a gene encoding a Cupin 2 conserved barrel protein with an unknown function. The triphenylmethane reductase gene tmr and a truncated dihydrolipoamide dehydrogenase gene that is flanked by IS1071 and another putative insertion element were identified in accessory region L2. Subcloning of the pGNB1 tmr gene demonstrated that this gene is responsible for the observed crystal violet resistance phenotype and mediates decolorization of the triphenylmethane dyes crystal violet, malachite green, and basic fuchsin. Plasmid pGNB1 and the associated phenotype are transferable to the α-proteobacterium Sinorhizobium meliloti and the γ-proteobacterium Escherichia coli. This is the first report of a promiscuous IncP-1β plasmid isolated from the bacterial community from a wastewater treatment plant that harbors a triphenylmethane reductase gene. The pGNB1-encoded enzyme activity is discussed with respect to bioremediation of sewage polluted with triphenylmethane dyes.

Rehabilitation and Upgrading of the Beni Suef City Wastewater Treatment Plant

1990 ◽  
Vol 22 (7-8) ◽  
pp. 131-138
Author(s):  
Ahmed Fadel
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Primary Treatment ◽  
High Rate ◽  
Shock Load ◽  
Economic Evaluations ◽  
Activated Sludge Process ◽  
Trickling Filter

Many of Egypt's cities have existing treatment plants under operation that have been constructed before 1970. Almost all of these treatment plants now need rehabilitation and upgrading to extend their services for a longer period. One of these plants is the Beni Suef City Wastewater Treatment Plant. The Beni Suef WWTP was constructed in 1956. It has primary treatment followed by secondary treatment employing intermediate rate trickling filters. The BOD, COD, and SS concentration levels are relatively high. They are approximately 800, 1100, and 600 mg/litre, respectively. The Beni Suef city required the determination of the level of work needed for the rehabilitation and upgrading of the existing 200 l/s plant and to extend its capacity to 440 l/s at year 2000 A description of the existing units, their deficiencies and operation problems, and the required rehabilitation are presented and discussed in this paper. Major problems facing the upgrading were the lack of space for expansion and the shortage of funds. It was, therefore, necessary to study several alternative solutions and methods of treatment. The choice of alternatives was from one of the following schemes: a) changing the filter medium, its mode of operation and increasing the number of units, b) changing the trickling filter to high rate and combining it with the activated sludge process, for operation by one of several possible combinations such as: trickling filter-solids contact, roughing filter-activated sludge, and trickling filter-activated sludge process, c) dividing the flow into two parts, the first part to be treated using the existing system and the second part to be treated by activated sludge process, and d) expanding the existing system by increasing the numbers of the different process units. The selection of the alternative was based on technical, operational and economic evaluations. The different alternatives were compared on the basis of system costs, shock load handling, treatment plant operation and predicted effluent quality. The flow schemes for the alternatives are presented. The methodology of selecting the best alternative is discussed. From the study it was concluded that the first alternative is the most reliable from the point of view of costs, handling shock load, and operation.

Operational Results of the Wolfsburg Wastewater Treatment Plant

1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Removal ◽  
Total Nitrogen Content ◽  
Activated Sludge Process

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.

Management of toxicity effects in a large wastewater treatment plant

1997 ◽  
Vol 36 (2-3) ◽  
pp. 1-8 ◽  
Author(s):  
P. Grau ◽  
B. P. Da-Rin
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Severe Case ◽  
Treatment Efficiency ◽  
Filamentous Bulking ◽  
Biological Phenomena ◽  
Inhibition Of Nitrification ◽  
Toxicity Effects

An unusually severe case of toxicity accompanied by activated sludge filamentous bulking was observed at the wastewater treatment plant Sao Paulo-Barueri. Treatment efficiency of the plant, operated without major problems for more than five years before, was significantly hindered for almost six months. Occurrence of toxic shocks was confirmed partly directly but mostly indirectly by inhibition of nitrification and biological phenomena related to toxicity. Several measures adopted, including the recycled activated sludge chlorination, are described in the paper.

Automatic control for DO and pH in the activated sludge process in a coke wastewater treatment plant

1998 ◽  
Vol 37 (12) ◽  
pp. 141-148 ◽  
Author(s):  
B. K. Lee ◽  
S. W. Sung ◽  
H. D. Chun ◽  
J. K. Koo
Keyword(s):  
Wastewater Treatment ◽  
Control System ◽  
Activated Sludge ◽  
Automatic Control ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Pi Controller ◽  
Activated Sludge Process ◽  
Set Point ◽  
Coke Wastewater

The objective of this study is to develop an automatic control system for dissolved oxygen (DO) and pH of the activated sludge process in a coke wastewater treatment plant. A discrete type autotuned proportional-integral (PI) controller using an auto-regressive exogenous (ARX) model as a process model was developed to maintain the DO concentration in aerators by controlling the speed of surface aerators. Also a nonlinear pH controller using the titration curve was used to control the pH of influent wastewater. This control system was tested in a pilot scale plant. During this pilot plant experiment, there was small deviation of pH and the electric power consumption of surface aerators was reduced up to 70% with respect to the full operation when the DO set point was 2 mg/l. For real plant operation with this system, the discrete PI controller showed good tracking for set point change. The electricity saving was more than 40% of the electricity consumption when considering surface aerators. As a result of maintaining the DO constantly at the set point by the automatic control system, the fluctuation of effluent quality was decreased and overall improvement of the effluent water quality was achieved.

Savings with upgraded performance through improved activated sludge denitrification in the combined activated sludge–biofilter system of the Southpest Wastewater Treatment Plant

2008 ◽  
Vol 57 (8) ◽  
pp. 1287-1293 ◽  
Author(s):  
A. Jobbágy ◽  
G. M. Tardy ◽  
Gy. Palkó ◽  
A. Benáková ◽  
O. Krhutková ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Biological Treatment ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Process Efficiency ◽  
Initial Value ◽  
Initial Profile ◽  
Biological Treatment System

The purpose of the experiments was to increase the rate of activated sludge denitrification in the combined biological treatment system of the Southpest Wastewater Treatment Plant in order to gain savings in cost and energy and improve process efficiency. Initial profile measurements revealed excess denitrification capacity of the preclarified wastewater. As a consequence, flow of nitrification filter effluent recirculated to the anoxic activated sludge basins was increased from 23,000 m3 d−1 to 42,288 m3 d−1 at an average preclarified influent flow of 64,843 m3 d−1, Both simulation studies and microbiological investigations suggested that activated sludge nitrification, achieved despite the low SRT (2–3 days), was initiated by the backseeding from the nitrification filters and facilitated by the decreased oxygen demand of the influent organics used for denitrification. With the improved activated sludge denitrification, methanol demand could be decreased to about half of the initial value. With the increased efficiency of the activated sludge pre-denitrification, plant effluent COD levels decreased from 40–70 mg l−1 to < 30–45 mg l−1 due to the decreased likelihood of methanol overdosing in the denitrification filter

Evaluating the treatment performance of a full scale Activated Sludge Plant in Islamabad, Pakistan

2012 ◽  
Vol 7 (1) ◽  
Author(s):  
S. S. Fatima ◽  
S. Jamal Khan
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Suspended Solids ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Full Scale ◽  
Operational Parameter ◽  
Mixed Liquor ◽  
Treatment Performance

In this study, the performance of wastewater treatment plant located at sector I-9 Islamabad, Pakistan, was evaluated. This full scale domestic wastewater treatment plant is based on conventional activated sludge process. The parameters which were monitored regularly included total suspended solids (TSS), mixed liquor suspended solids (MLSS), mixed liquor volatile suspended solids (MLVSS), biological oxygen demand (BOD), and chemical oxygen demand (COD). It was found that the biological degradation efficiency of the plant was below the desired levels in terms of COD and BOD. Also the plant operators were not maintaining consistent sludge retention time (SRT). Abrupt discharge of MLSS through the Surplus Activated sludge (SAS) pump was the main reason for the low MLSS in the aeration tank and consequently low treatment performance. In this study the SRT was optimized based on desired MLSS concentration between 3,000–3,500 mg/L and required performance in terms of BOD, COD and TSS. This study revealed that SRT is a very important operational parameter and its knowledge and correct implementation by the plant operators should be mandatory.

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