scholarly journals Complex Class 1 Integron CarryingqnrB62andblaVIM-2in a Citrobacter freundii Clinical Isolate

2016 ◽  
Vol 60 (11) ◽  
pp. 6937-6940 ◽  
Author(s):  
Jae Jin Lee ◽  
Mi-Na Kim ◽  
Kwang Seung Park ◽  
Jung Hun Lee ◽  
Asad Mustafa Karim ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Efflux Pump ◽  
Gene Array ◽  
Complex Class ◽  
Repeat Region ◽  
Inverted Repeat Region ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Genetic Context

ABSTRACTThe coexistence ofqnrB62andblaVIM-2was detected in aCitrobacterclinical isolate. The reduced fluoroquinolone susceptibility is attributable toqnrB62, mutations of quinolone-resistance-determining regions, and an efflux pump or pumps. The genetic context surrounding chromosomalqnrB62was a novel complex class 1 integron (In1184::ISCR1::qnrB62) containing a unique gene array (blaVIM-2-aacA4′-8-gucD). An 18-nucleotide deletion at the 3′ end of thepspAgene [pspA(Δ18)], upstream ofqnrB62, and an inverted repeat region (IRR2) were detected in In1184::ISCR1::qnrB62, indicating past transposition events.

scholarly journals Molecular Epidemiology and Genetic Characteristics of VariousblaPERGenes in Shanghai, China

2016 ◽  
Vol 60 (6) ◽  
pp. 3849-3853 ◽  
Author(s):  
Lianyan Xie ◽  
Jun Wu ◽  
Fangfang Zhang ◽  
Lizhong Han ◽  
Xiaokui Guo ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Inverted Repeat ◽  
Transmission Mechanism ◽  
The Other ◽  
Complex Class ◽  
Class 1 Integron ◽  
Genetic Characteristics ◽  
Gram Negative ◽  
Content Type ◽  
Class 1

We describe the genetic characteristics and possible transmission mechanism ofblaPERin 25 clinical Gram-negative bacilli in Shanghai.blaPER, includingblaPER-1,blaPER-3, andblaPER-4, was located chromosomally or in different plasmids. Tn1213harboringblaPER-1was first identified in twoProteus mirabilisisolates in China. The otherblaPERvariants were preceded by an ISCR1element inside the complex class 1 integron associated with IS26, Tn21, Tn1696, and a miniature inverted-repeat transposable element.

scholarly journals Klebsiella pneumoniae Isolate from a New York City Hospital Belonging to Sequence Type 258 and CarryingblaKPC-2andblaVIM-4

2016 ◽  
Vol 60 (3) ◽  
pp. 1924-1927 ◽  
Author(s):  
Mariana Castanheira ◽  
Lalitagauri M. Deshpande ◽  
Janet C. Mills ◽  
Ronald N. Jones ◽  
Rosemary Soave ◽  
...  
Keyword(s):  
New York ◽  
New York City ◽  
Klebsiella Pneumoniae ◽  
Efflux Pump ◽  
Sequence Type ◽  
City Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Elevated Expression

Among 69 of 139 (49.6%) carbapenem-nonsusceptibleEnterobacteriaceaecarryingblaKPC, 1Klebsiella pneumoniaewas also positive forblaVIM. The isolate belonged to sequence type 258 (ST258) and carriedblaKPC-2on a copy ofTn4401a andblaVIM-4on a class 1 integron. Genes were located on distinct plasmids belonging to Inc types A/C and FII. Elevated expression of the efflux pump AcrAB-TolC (acrA, 15.3 times) and reduced expression of outer membrane protein genesompK35andompK37(0.16 and 0.081 times, respectively) associated with various amino acid alterations on OmpK37 were observed. The presence of two carbapenemases in ST258K. pneumoniaeis of great concern due to the ability of this organism to widely disseminate.

scholarly journals Emergence of Escherichia coli Sequence Type ST131 Carrying both theblaGES-5andblaCTX-M-15Genes

2011 ◽  
Vol 55 (6) ◽  
pp. 2974-2975 ◽  
Author(s):  
Juwon Kim ◽  
Seong Geun Hong ◽  
Il Kwon Bae ◽  
Ji Roung Kang ◽  
Seok Hoon Jeong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
High Level ◽  
Level Resistance

ABSTRACTEscherichia coliclinical isolate BD07372 of sequence type ST131 recovered from a bed sore specimen exhibited high-level resistance to ceftazidime and cefotaxime but exhibited susceptibility to imipenem and meropenem. The isolate harbored two β-lactamase genes, theblaCTX-M-15gene carried by an ∼250-kbp plasmid carrying the FIA and FIC replicons and theblaGES-5gene carried by a class 1 integron in the chromosome.

scholarly journals SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate

2011 ◽  
Vol 55 (11) ◽  
pp. 5143-5149 ◽  
Author(s):  
Jun-ichi Wachino ◽  
Hiroyuki Yoshida ◽  
Kunikazu Yamane ◽  
Satowa Suzuki ◽  
Mari Matsui ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolate ◽  
Apple Orchard ◽  
Resistance Pattern ◽  
Binding Motif ◽  
Class 1 Integron ◽  
Uncultured Bacterium ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTA carbapenem-resistantSerratia marcescensstrain, 10mdr148, was identified in a Japanese hospital in 2010. The carbapenem resistance of this strain was attributed to the production of a novel metallo-β-lactamase (MBL), named SMB-1 (Serratiametallo-β-lactamase). SMB-1 possessed a zinc binding motif, H(Q)XHXDH (residues 116 to 121), H196, and H263 and was categorized as a member of subclass B3 MBL. SMB-1 has 75% amino acid identity with the most closely related MBL, AMO1, of uncultured bacterium, recently identified through the metagenomic analysis of apple orchard soil. The introduction ofblaSMB-1intoEscherichia coliconferred resistance to a variety of β-lactam antibiotics, penicillins, cephalosporins, and carbapenems, but not aztreonam, a resistance pattern consistent with those of other MBLs. SMB-1 demonstrated highkcatvalues of >500 s−1for carbapenems, resulting in the highest hydrolyzing efficiency (kcat/Km) among the agents tested. The hydrolyzing activity of SMB-1 was well inhibited by chelating agents. TheblaSMB-1gene was located on the chromosome ofS. marcescensstrain 10mdr148 and at the 3′ end of the ISCR1element in complex with a typical class 1 integron carryingaac(6′)-IbandcatB3gene cassettes. Downstream ofblaSMB-1, the second copy of the 3′conserved segment and ISCR1were found. To our knowledge, this is the first subclass B3 MBL gene associated with an ISCR1element identified in anEnterobacteriaceaeclinical isolate. A variety of antibiotic resistance genes embedded with ISCR1have been widely spread amongEnterobacteriaceaeclinical isolates, thus the further dissemination ofblaSMB-1mediated by ISCR1transposition activity may become a future concern.

scholarly journals PGI2 Is a Novel SGI1-Relative Multidrug-Resistant Genomic Island Characterized in Proteus mirabilis

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Chang-Wei Lei ◽  
Yan-Peng Chen ◽  
Ling-Han Kong ◽  
Jin-Xin Zeng ◽  
Yong-Xiang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Proteus Mirabilis ◽  
Genomic Island ◽  
Multidrug Resistant ◽  
Complex Class ◽  
Helper Plasmid ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACT A novel 61,578-bp genomic island named Proteus genomic island 2 (PGI2) was characterized in Proteus mirabilis of swine origin in China. The 23.85-kb backbone of PGI2 is related to those of Salmonella genomic island 1 and Acinetobacter genomic island 1. The multidrug resistance (MDR) region of PGI2 is a complex class 1 integron containing 14 different resistance genes. PGI2 was conjugally mobilized in trans to Escherichia coli in the presence of a conjugative IncC helper plasmid.

scholarly journals Novel Complex Class 1 Integron Bearing an ISCR1 Element in an Escherichia coli Isolate Carrying the blaCTX-M-14 Gene

2007 ◽  
Vol 51 (8) ◽  
pp. 3017-3019 ◽  
Author(s):  
Il Kwon Bae ◽  
You-Nae Lee ◽  
Wee Gyo Lee ◽  
Sang Hee Lee ◽  
Seok Hoon Jeong
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Complex Class ◽  
Class 1 Integron ◽  
Coli Isolate ◽  
Gene Cassettes ◽  
Class 1 ◽  
Novel Complex ◽  
M 14

ABSTRACT This work identifies an ISCR1-related bla CTX-M-14 gene, which has never been reported before, from a clinical isolate of Escherichia coli. The bla CTX-M-14 gene was preceded by an ISCR1 element that was followed by a class 1 integron containing three different insert gene cassettes, i.e., dfrA12, orfF, and aadA2.

scholarly journals Complete Nucleotide Sequence of a Conjugative Plasmid CarryingblaPER-1

2015 ◽  
Vol 59 (6) ◽  
pp. 3582-3584 ◽  
Author(s):  
Ruichao Li ◽  
Marcus Ho Yin Wong ◽  
Yuanjie Zhou ◽  
Edward Wai-chi Chan ◽  
Sheng Chen
Keyword(s):  
Multidrug Resistance ◽  
Nucleotide Sequence ◽  
Complete Nucleotide Sequence ◽  
Vibrio Parahaemolyticus ◽  
Conjugative Plasmid ◽  
Complex Class ◽  
Class 1 Integron ◽  
Content Type ◽  
Transmissible Plasmid ◽  
Class 1

ABSTRACTThe nucleotide sequence of a self-transmissible plasmid pVPH1 harboringblaPER-1fromVibrio parahaemolyticuswas determined. pVPH1 was 183,730 bp in size and shared a backbone similar to pAQU1 and pAQU2, differing mainly in an ∼40-kb multidrug resistance (MDR) region. A complex class 1 integron was identified together with ISCR1andblaPER-1(ISCR1-blaPER-1-gst-abct-qacEΔ1-sul1), which was shown to form a circular intermediate playing an important role in the dissemination ofblaPER-1.

Carriage of qnrA1 and qnrB2, blaCTX-M15, and complex class 1 integron in a clinical multiresistant Citrobacter freundii isolate

2010 ◽  
Vol 67 (2) ◽  
pp. 188-190 ◽  
Author(s):  
Sónia Ferreira ◽  
Ana Paradela ◽  
Jorge Velez ◽  
Elmano Ramalheira ◽  
Timothy R. Walsh ◽  
...  
Keyword(s):  
Citrobacter Freundii ◽  
Complex Class ◽  
Class 1 Integron ◽  
Class 1

scholarly journals Mobile Genetic Elements Related to the Diffusion of Plasmid-Mediated AmpC β-Lactamases or Carbapenemases from Enterobacteriaceae: Findings from a Multicenter Study in Spain

2015 ◽  
Vol 59 (9) ◽  
pp. 5260-5266 ◽  
Author(s):  
L. Zamorano ◽  
E. Miró ◽  
C. Juan ◽  
L. Gómez ◽  
G. Bou ◽  
...  
Keyword(s):  
Southern Hybridization ◽  
Mobile Genetic Elements ◽  
Pulsed Field Gel Electrophoresis ◽  
Content Type ◽  
Genetic Elements ◽  
Chromosomal Origin ◽  
Class 1 ◽  
Rapid Dissemination ◽  
Large Plasmids ◽  
Genetic Context

ABSTRACTWe examined the genetic context of 74 acquiredampCgenes and 17 carbapenemase genes from 85 of 640Enterobacteriaceaeisolates collected in 2009. Using S1 pulsed-field gel electrophoresis and Southern hybridization, 37 of 74blaAmpCgenes were located on large plasmids of different sizes belonging to six incompatibility groups. We used sequencing and PCR mapping to investigate the regions flanking the acquiredampCgenes. TheblaCMY-2-like genes were associated with ISEcp1; the surroundingblaDHAgenes were similar toKlebsiella pneumoniaeplasmid pTN60013 associated with IS26and thepspandsapoperons; and theblaACC-1genes were associated with IS26elements inserted into ISEcp1. All of the carbapenemase genes (blaVIM-1,blaIMP-22, andblaIMP-28) were located in class 1 integrons. Therefore, although plasmids are the main cause of the rapid dissemination ofampCgenes amongEnterobacteriaceae, we need to be aware that other mobile genetic elements, such as insertion sequences, transposons, or integrons, can be involved in the mobilization of these genes of chromosomal origin. Additionally, three new integrons (In846 to In848) are described in this study.

Sign in / Sign up

Export Citation Format

Share Document