scholarly journals SMB-1, a Novel Subclass B3 Metallo-β-Lactamase, Associated with ISCR1and a Class 1 Integron, from a Carbapenem-Resistant Serratia marcescens Clinical Isolate

2011 ◽  
Vol 55 (11) ◽  
pp. 5143-5149 ◽  
Author(s):  
Jun-ichi Wachino ◽  
Hiroyuki Yoshida ◽  
Kunikazu Yamane ◽  
Satowa Suzuki ◽  
Mari Matsui ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Clinical Isolate ◽  
Apple Orchard ◽  
Resistance Pattern ◽  
Binding Motif ◽  
Class 1 Integron ◽  
Uncultured Bacterium ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTA carbapenem-resistantSerratia marcescensstrain, 10mdr148, was identified in a Japanese hospital in 2010. The carbapenem resistance of this strain was attributed to the production of a novel metallo-β-lactamase (MBL), named SMB-1 (Serratiametallo-β-lactamase). SMB-1 possessed a zinc binding motif, H(Q)XHXDH (residues 116 to 121), H196, and H263 and was categorized as a member of subclass B3 MBL. SMB-1 has 75% amino acid identity with the most closely related MBL, AMO1, of uncultured bacterium, recently identified through the metagenomic analysis of apple orchard soil. The introduction ofblaSMB-1intoEscherichia coliconferred resistance to a variety of β-lactam antibiotics, penicillins, cephalosporins, and carbapenems, but not aztreonam, a resistance pattern consistent with those of other MBLs. SMB-1 demonstrated highkcatvalues of >500 s−1for carbapenems, resulting in the highest hydrolyzing efficiency (kcat/Km) among the agents tested. The hydrolyzing activity of SMB-1 was well inhibited by chelating agents. TheblaSMB-1gene was located on the chromosome ofS. marcescensstrain 10mdr148 and at the 3′ end of the ISCR1element in complex with a typical class 1 integron carryingaac(6′)-IbandcatB3gene cassettes. Downstream ofblaSMB-1, the second copy of the 3′conserved segment and ISCR1were found. To our knowledge, this is the first subclass B3 MBL gene associated with an ISCR1element identified in anEnterobacteriaceaeclinical isolate. A variety of antibiotic resistance genes embedded with ISCR1have been widely spread amongEnterobacteriaceaeclinical isolates, thus the further dissemination ofblaSMB-1mediated by ISCR1transposition activity may become a future concern.

scholarly journals Coproduction of KPC-2 and IMP-10 in Carbapenem-Resistant Serratia marcescens Isolates from an Outbreak in a Brazilian Teaching Hospital

2015 ◽  
Vol 53 (7) ◽  
pp. 2324-2328 ◽  
Author(s):  
Kesia Esther Silva ◽  
Rodrigo Cayô ◽  
Cecilia Godoy Carvalhaes ◽  
Flávia Patussi Correia Sacchi ◽  
Fernanda Rodrigues-Costa ◽  
...  
Keyword(s):  
Antimicrobial Agent ◽  
Serratia Marcescens ◽  
Teaching Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class 1

We describe an outbreak caused by KPC-2- and IMP-10-producingSerratia marcescensisolates in a Brazilian teaching hospital. Tigecycline was the only active antimicrobial agent tested. TheblaIMP-10gene was located in a new class 1 integron, namedIn990, carried by a nonconjugative plasmid, in contrast toblaKPC-2.

scholarly journals A Multispecies Cluster of VIM-1 Carbapenemase-Producing Enterobacterales Linked by a Novel, Highly Conjugative, and Broad-Host-Range IncA Plasmid Forebodes the Reemergence of VIM-1

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Gabriele Arcari ◽  
Federica Maria Di Lella ◽  
Giulia Bibbolino ◽  
Fabio Mengoni ◽  
Marzia Beccaccioli ◽  
...  
Keyword(s):  
Host Range ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Gene Cassette ◽  
University Hospital ◽  
Broad Host Range ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACT In this study, we investigated VIM-1-producing Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii, and Enterobacter cloacae strains, isolated in 2019 during a period of active surveillance of carbapenem-resistant Enterobacterales in a large university hospital in Italy. VIM-1-producing strains colonized the gut of patients, with up to three different VIM-1-positive bacterial species isolated from a single rectal swab, but also caused bloodstream infection in one colonized patient. In the multispecies cluster, blaVIM-1 was identified in a 5-gene cassette class 1 integron, associated with several genetic determinants, including the blaSHV-12, qnrS1, and mph(A) genes, located on a highly conjugative and broad-host-range IncA plasmid. The characteristics and origin of this IncA plasmid were studied.

scholarly journals Complex Class 1 Integron CarryingqnrB62andblaVIM-2in a Citrobacter freundii Clinical Isolate

2016 ◽  
Vol 60 (11) ◽  
pp. 6937-6940 ◽  
Author(s):  
Jae Jin Lee ◽  
Mi-Na Kim ◽  
Kwang Seung Park ◽  
Jung Hun Lee ◽  
Asad Mustafa Karim ◽  
...  
Keyword(s):  
Clinical Isolate ◽  
Efflux Pump ◽  
Gene Array ◽  
Complex Class ◽  
Repeat Region ◽  
Inverted Repeat Region ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Genetic Context

ABSTRACTThe coexistence ofqnrB62andblaVIM-2was detected in aCitrobacterclinical isolate. The reduced fluoroquinolone susceptibility is attributable toqnrB62, mutations of quinolone-resistance-determining regions, and an efflux pump or pumps. The genetic context surrounding chromosomalqnrB62was a novel complex class 1 integron (In1184::ISCR1::qnrB62) containing a unique gene array (blaVIM-2-aacA4′-8-gucD). An 18-nucleotide deletion at the 3′ end of thepspAgene [pspA(Δ18)], upstream ofqnrB62, and an inverted repeat region (IRR2) were detected in In1184::ISCR1::qnrB62, indicating past transposition events.

scholarly journals Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

2012 ◽  
Vol 56 (5) ◽  
pp. 2746-2749 ◽  
Author(s):  
Sanda Sardelic ◽  
Branka Bedenic ◽  
Céline Colinon-Dupuich ◽  
Stjepan Orhanovic ◽  
Zrinka Bosnjak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Coastal Region ◽  
Large Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Variable Part ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Sequence Types

ABSTRACTOne hundred sixty-nine nonreplicate imipenem-resistantPseudomonas aeruginosastrains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried theblaVIM-2gene located in a novel class 1 integron composed in its variable part of theblaVIM-2-blaoxa-10-ΔqacF-aacA4genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.

scholarly journals Isolation of an Integron-Borne blaVIM-4 Type Metallo-β-Lactamase Gene from a Carbapenem-Resistant Pseudomonas aeruginosa Clinical Isolate in Hungary

2004 ◽  
Vol 48 (9) ◽  
pp. 3576-3578 ◽  
Author(s):  
Balázs Libisch ◽  
Mária Gacs ◽  
Károly Csiszár ◽  
Mónika Muzslay ◽  
László Rókusz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Structural Similarity ◽  
Class 1 Integron ◽  
Carbapenem Resistant ◽  
High Structural Similarity ◽  
Class 1 ◽  
Lactamase Gene

ABSTRACT The first integron-borne metallo-β-lactamase gene was isolated in Hungary. The bla VIM-4 gene is located on a class 1 integron that also carries a novel bla OXA-like gene. The integron is harbored by a serotype O12 Pseudomonas aeruginosa strain and shows high structural similarity to integrons isolated in Greece and Poland.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals Emergence of Escherichia coli Sequence Type ST131 Carrying both theblaGES-5andblaCTX-M-15Genes

2011 ◽  
Vol 55 (6) ◽  
pp. 2974-2975 ◽  
Author(s):  
Juwon Kim ◽  
Seong Geun Hong ◽  
Il Kwon Bae ◽  
Ji Roung Kang ◽  
Seok Hoon Jeong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Isolate ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
High Level ◽  
Level Resistance

ABSTRACTEscherichia coliclinical isolate BD07372 of sequence type ST131 recovered from a bed sore specimen exhibited high-level resistance to ceftazidime and cefotaxime but exhibited susceptibility to imipenem and meropenem. The isolate harbored two β-lactamase genes, theblaCTX-M-15gene carried by an ∼250-kbp plasmid carrying the FIA and FIC replicons and theblaGES-5gene carried by a class 1 integron in the chromosome.

scholarly journals OXA-198, an Acquired Carbapenem-Hydrolyzing Class D β-Lactamase from Pseudomonas aeruginosa

2011 ◽  
Vol 55 (10) ◽  
pp. 4828-4833 ◽  
Author(s):  
Farid El Garch ◽  
Pierre Bogaerts ◽  
Carine Bebrone ◽  
Moreno Galleni ◽  
Youri Glupczynski
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Carbapenem Resistance ◽  
Amino Acid Identity ◽  
Ventilator Associated Pneumonia ◽  
Class 1 Integron ◽  
Content Type ◽  
New Class ◽  
Carbapenem Resistant ◽  
Class D ◽  
Class 1

ABSTRACTA carbapenem-resistantPseudomonas aeruginosastrain (PA41437) susceptible to expanded-spectrum cephalosporins was recovered from several consecutive lower-respiratory-tract specimens of a patient who developed a ventilator-associated pneumonia while hospitalized in an intensive care unit. Cloning experiments identified OXA-198, a new class D β-lactamase which was weakly related (less than 45% amino acid identity) to other class D β-lactamases. Expression inEscherichia coliTOP10 and inP. aeruginosaPAO1 led to transformants that were resistant to ticarcillin and showed reduced susceptibility to carbapenems and cefepime. TheblaOXA-198gene was harbored by a class 1 integron carried by a ca. 46-kb nontypeable plasmid. This study describes a novel class D β-lactamase involved in carbapenem resistance inP. aeruginosa.

scholarly journals First Report ofblaIMP-8in Raoultella planticola

2013 ◽  
Vol 58 (1) ◽  
pp. 593-595 ◽  
Author(s):  
Sung-Pin Tseng ◽  
Jann-Tay Wang ◽  
Chih-Yuan Liang ◽  
Pei-Shan Lee ◽  
Yee-Chun Chen ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Protein Loss ◽  
Class 1 Integron ◽  
Raoultella Planticola ◽  
Content Type ◽  
First Report ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTwo carbapenem-resistantRaoultella planticolaclinical isolates were isolated from patients with pneumonia and Port-A catheter-related bacteremia, respectively, in Taiwan. These isolates remained susceptible to fluoroquinolone, aminoglycoside, and colistin. Though the two isolates had the same antibiogram, plasmidic carbapenemaseblaIMP-8, class 1 integron cassette (dfrA12-orfF-aadA2), andqnrB2, they had different pulsed-field gel electrophoresis patterns, plasmid sizes, and outer membrane protein loss profiles. To our knowledge, this is the first report ofblaIMP-8found inR. planticola. Interestingly,blaIMP-8is the most common carbapenemase found inKlebsiella pneumoniaein Taiwan. In the literature, carbapenemase genes inR. planticolain each country were also found in carbapenem-resistantEnterobacteriaceaein the same country.

scholarly journals Clinical Carbapenem-Resistant Acinetobacter baylyi Strain CoharboringblaSIM-1andblaOXA-23from China

2011 ◽  
Vol 55 (11) ◽  
pp. 5347-5349 ◽  
Author(s):  
Zhihui Zhou ◽  
Xiaoxing Du ◽  
Li Wang ◽  
Qing Yang ◽  
Yiqi Fu ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Acinetobacter Baumannii ◽  
Clonal Complex ◽  
Gene Cassette ◽  
Large Plasmid ◽  
Acinetobacter Baylyi ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTblaSIM-1andblaOXA-23were codetected in clinical carbapenem-resistantAcinetobacter baylyistrain NB09A30. Both of carbapenemase genes were located on a large plasmid (ca. 360 kb).blaSIM-1was found as a gene cassette inserted into a class 1 integron identical to that determined inAcinetobactersp. isolates from South Korea. The genetic structure ofblaOXA-23in NB09A30 was different from that in the prevalentAcinetobacter baumanniiof clonal complex 92 (CC92) from the same hospital.

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