scholarly journals Molecular Characterization ofblaNDM-1in a Sequence Type 235 Pseudomonas aeruginosa Isolate from France

2013 ◽  
Vol 57 (7) ◽  
pp. 3408-3411 ◽  
Author(s):  
Frédéric Janvier ◽  
Katy Jeannot ◽  
Sophie Tessé ◽  
Marjorie Robert-Nicoud ◽  
Hervé Delacour ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Insertion Sequence ◽  
Variable Region ◽  
Sequence Type ◽  
Common Region ◽  
Class 1 Integron ◽  
Genetic Studies ◽  
Content Type ◽  
Class 1 ◽  
Previous Hospitalization

ABSTRACTAn NDM-1 carbapenemase-producingPseudomonas aeruginosaisolate was recovered from a patient hospitalized in France after a previous hospitalization in Serbia. Genetic studies revealed that theblaNDM-1gene was surrounded by insertion sequence ISAba125and a truncated bleomycin resistance gene. ThisblaNDM-1region was a part of the variable region of a new complex class 1 integron bearing IS common region 1 (ISCR1). The presence of ISPa7upstream of this integron suggests insertion in a chromosomally located Tn402-like structure.

scholarly journals Early Strains of Multidrug-Resistant Salmonella enterica Serovar Kentucky Sequence Type 198 from Southeast Asia Harbor Salmonella Genomic Island 1-J Variants with a Novel Insertion Sequence

2012 ◽  
Vol 56 (10) ◽  
pp. 5096-5102 ◽  
Author(s):  
Simon Le Hello ◽  
François-Xavier Weill ◽  
Véronique Guibert ◽  
Karine Praud ◽  
Axel Cloeckaert ◽  
...  
Keyword(s):  
Southeast Asia ◽  
Salmonella Enterica ◽  
Insertion Sequence ◽  
Genomic Island ◽  
Far East ◽  
Gene Clusters ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACTSalmonellagenomic island 1 (SGI1) is a 43-kb integrative mobilizable element that harbors a great diversity of multidrug resistance gene clusters described in numerousSalmonella entericaserovars and also inProteus mirabilis. The majority of SGI1 variants contain an In104-derivative complex class 1 integron inserted between resolvase generesand open reading frame (ORF) S044 in SGI1. Recently, the international spread of ciprofloxacin-resistantS. entericaserovar Kentucky sequence type 198 (ST198) containing SGI1-K variants has been reported. A retrospective study was undertaken to characterize ST198S. Kentucky strains isolated before the spread of the epidemic ST198-SGI1-K population in Africa and the Middle East. Here, we characterized 12 ST198S. Kentucky strains isolated between 1969 and 1999, mainly from humans returning from Southeast Asia (n= 10 strains) or Israel (n= 1 strain) or from meat in Egypt (n= 1 strain). All these ST198S. Kentucky strains did not belong to the XbaI pulsotype X1 associated with the African epidemic clone but to pulsotype X2. SGI1-J subgroup variants containing different complex integrons with a partial transposition module and inserted within ORF S023 of SGI1 were detected in six strains. The SGI1-J4 variant containing a partially deleted class 1 integron and thus showing a narrow resistance phenotype to sulfonamides was identified in two epidemiologically unrelated strains from Indonesia. The four remaining strains harbored a novel SGI1-J variant, named SGI1-J6, which containedaadA2,floR2,tetR(G)-tetA(G), andsul1resistance genes within its complex integron. Moreover, in all theseS. Kentucky isolates, a novel insertion sequence related to the IS630family and named ISSen5was found inserted upstream of the SGI1 complex integron in ORF S023. Thus, two subpopulations ofS. Kentucky ST198 independently and exclusively acquired the SGI1 during the 1980s and 1990s. Unlike the ST198-X1 African epidemic subpopulation, the ST198-X2 subpopulation mainly from Asia harbors variants of the SGI1-J subgroup that are encountered mainly in the Far East, as previously described forS. entericaserovars Emek and Virchow.

scholarly journals First Report ofblaIMP-14on a Plasmid Harboring Multiple Drug Resistance Genes in Escherichia coli Sequence Type 131

2016 ◽  
Vol 60 (8) ◽  
pp. 5068-5071 ◽  
Author(s):  
Nicole Stoesser ◽  
Anna E. Sheppard ◽  
Gisele Peirano ◽  
Robert P. Sebra ◽  
Tarah Lynch ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Multiple Drug Resistance ◽  
Carbapenem Resistance ◽  
Sequence Type ◽  
Multiple Drug ◽  
Class 1 Integron ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Class 1

ABSTRACTTheblaIMP-14carbapenem resistance gene has largely previously been observed inPseudomonas aeruginosaandAcinetobacterspp. As part of global surveillance and sequencing of carbapenem-resistantEscherichia coli, we identified a sequence type 131 strain harboringblaIMP-14within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C2plasmid. The emergence ofblaIMP-14in this context in the ST131 lineage is of potential clinical concern.

scholarly journals Dissemination of High-Risk Clones of Extensively Drug-Resistant Pseudomonas aeruginosa in Colombia

2015 ◽  
Vol 59 (4) ◽  
pp. 2421-2425 ◽  
Author(s):  
Adriana Correa ◽  
Rosa del Campo ◽  
Marcela Perenguez ◽  
Victor M. Blanco ◽  
Mercedes Rodríguez-Baños ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
High Risk ◽  
Sequence Type ◽  
Single Locus ◽  
Drug Resistant ◽  
Class 1 Integron ◽  
Content Type ◽  
Extensively Drug Resistant ◽  
Single Locus Variant ◽  
Class 1

ABSTRACTThe ability ofPseudomonas aeruginosato develop resistance to most antimicrobials represents an important clinical threat worldwide. We report the dissemination in several Colombian hospitals of two predominant lineages of extensively drug-resistant (XDR) carbapenemase-producingP. aeruginosastrains. These lineages belong to the high-risk clones sequence type 111 (ST111) and ST235 and harborblaVIM-2on a class 1 integron andblaKPC-2on a Tn4401transposon, respectively. Additionally,P. aeruginosaST1492, a novel single-locus variant of ST111, was identified. Clonal dissemination and the presence of mobile genetic elements likely explain the successful spread of XDRP. aeruginosastrains in Colombia.

scholarly journals Emergence of Pseudomonas aeruginosa with class 1 integron carrying blaVIM-2 and blaVIM-4 in the University Clinical Hospital of Bialystok (northeastern Poland)

2017 ◽  
Vol 71 (1) ◽  
pp. 0-0 ◽  
Author(s):  
Anna Michalska-Falkowska ◽  
Paweł Tomasz Sacha ◽  
Henryk Grześ ◽  
Tomasz Hauschild ◽  
Piotr Wieczorek ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Resistance ◽  
Genetic Relatedness ◽  
Housekeeping Genes ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Clinical Hospital ◽  
Class 1 ◽  
Severe Infections ◽  
The University

The effectiveness of carbapenems, considered as last-resort antimicrobials in severe infections, becomes compromised by bacterial resistance. The production of metallo-β-lactamases (MBLs) is the most significant threat to carbapenems activity among Pseudomonas aeruginosa. The aim of this study was to assess the presence and type of MBLs genes in carbapenem-resistant P. aeruginosa clinical strains, to identify the location of MBLs genes and to determine genetic relatedness between MBL-producers using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST).The first identified MBL-positive (with blaVIM genes) P. aeruginosa strains were isolated from patients hospitalized in the University Clinical Hospital of Bialystok in the period from September 2012 to December 2013. Variants of MBLs genes and variable integron regions were characterized by PCR and sequencing. PFGE was performed after digesting of bacterial genomes by XbaI enzyme. By MLST seven housekeeping genes were analyzed for the determination of sequence type (ST). Three strains carried the blaVIM-2 gene and one harbored the blaVIM-4 gene. The blaVIM genes resided within class 1 integrons. PCR mapping of integrons revealed the presence of four different cassette arrays. Genetic relatedness analysis by PFGE classified VIM-positive strains into four unrelated pulsotypes (A–D). MLST demonstrated the presence of four (ST 111, ST27, and ST17) different sequence type including one previously undescribed new type of ST 2342. Antimicrobial susceptibility testing showed that VIM-positive strains were resistant to carbapenems, cephalosporins, aminoglycosides, and quinolones, intermediate to aztreonam, and susceptible only to colistin. Integrons mapping, PFGE, and MLST results may point to different origin of these strains and independent introduction into hospitalized patients.

Characterization of VIM-4 Producing Clinical Pseudomonas aeruginosa Isolates from Western Algeria: Sequence Type and Class 1 Integron Description

2020 ◽  
Vol 26 (12) ◽  
pp. 1437-1441
Author(s):  
Fatma Zohra Zaidi ◽  
Radia Dali-Yahia ◽  
Karima Zenati ◽  
Leila Yazi ◽  
Manon Lounes ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Class 1 ◽  
Western Algeria

scholarly journals Complete Genome Sequences of Two Pseudomonas aeruginosa Strains Isolated from Children with Bacteremia

2017 ◽  
Vol 5 (35) ◽  
Author(s):  
Luis F. Espinosa-Camacho ◽  
Gabriela Delgado ◽  
Guadalupe Miranda-Novales ◽  
Gloria Soberón-Chávez ◽  
Luis D. Alcaraz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Real Time ◽  
Mexico City ◽  
Single Molecule ◽  
Complete Genome ◽  
Genome Sequences ◽  
Class 1 Integron ◽  
Content Type ◽  
Pacbio Rs Ii ◽  
Class 1

ABSTRACT Two Pseudomonas aeruginosa strains isolated from children with bacteremia in Mexico City were sequenced using PacBio RS-II single-molecule real-time (SMRT) technology. The strains consist of a 7.0- to 7.4-Mb chromosome, with a high content of mobile elements, and variation in the genetic content of class 1 integron In1409.

scholarly journals Characterization of Metallo-β-Lactamase VIM-27, an A57S Mutant of VIM-1 Associated with Klebsiella pneumoniae ST147

2011 ◽  
Vol 55 (7) ◽  
pp. 3570-3572 ◽  
Author(s):  
C. C. Papagiannitsis ◽  
S. D. Kotsakis ◽  
E. Petinaki ◽  
A. C. Vatopoulos ◽  
E. Tzelepi ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Kinetic Parameters ◽  
Sequence Type ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1

ABSTRACTVIM-27 metallo-β-lactamase, an Ala57→ Ser variant of VIM-1, was identified in threeKlebsiella pneumoniaeisolates belonging to sequence type 147.blaVIM-27was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

scholarly journals Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

2012 ◽  
Vol 56 (5) ◽  
pp. 2746-2749 ◽  
Author(s):  
Sanda Sardelic ◽  
Branka Bedenic ◽  
Céline Colinon-Dupuich ◽  
Stjepan Orhanovic ◽  
Zrinka Bosnjak ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Coastal Region ◽  
Large Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Variable Part ◽  
Carbapenem Resistant ◽  
Class 1 ◽  
Sequence Types

ABSTRACTOne hundred sixty-nine nonreplicate imipenem-resistantPseudomonas aeruginosastrains isolated in a large hospital on the coastal region of Croatia were studied. The most active antibiotics were colistin and amikacin. Most of the isolates were multiresistant. The most prevalent serotype was O12, followed by O11. Six strains carried theblaVIM-2gene located in a novel class 1 integron composed in its variable part of theblaVIM-2-blaoxa-10-ΔqacF-aacA4genes. Metallo-β-lactamase-producing strains belonged to sequence types ST235 and ST111.

scholarly journals Klebsiella pneumoniae Isolate from a New York City Hospital Belonging to Sequence Type 258 and CarryingblaKPC-2andblaVIM-4

2016 ◽  
Vol 60 (3) ◽  
pp. 1924-1927 ◽  
Author(s):  
Mariana Castanheira ◽  
Lalitagauri M. Deshpande ◽  
Janet C. Mills ◽  
Ronald N. Jones ◽  
Rosemary Soave ◽  
...  
Keyword(s):  
New York ◽  
New York City ◽  
Klebsiella Pneumoniae ◽  
Efflux Pump ◽  
Sequence Type ◽  
City Hospital ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Elevated Expression

Among 69 of 139 (49.6%) carbapenem-nonsusceptibleEnterobacteriaceaecarryingblaKPC, 1Klebsiella pneumoniaewas also positive forblaVIM. The isolate belonged to sequence type 258 (ST258) and carriedblaKPC-2on a copy ofTn4401a andblaVIM-4on a class 1 integron. Genes were located on distinct plasmids belonging to Inc types A/C and FII. Elevated expression of the efflux pump AcrAB-TolC (acrA, 15.3 times) and reduced expression of outer membrane protein genesompK35andompK37(0.16 and 0.081 times, respectively) associated with various amino acid alterations on OmpK37 were observed. The presence of two carbapenemases in ST258K. pneumoniaeis of great concern due to the ability of this organism to widely disseminate.

scholarly journals Novel 6′-N-Aminoglycoside Acetyltransferase AAC(6′)-Iaj from a Clinical Isolate of Pseudomonas aeruginosa

2012 ◽  
Vol 57 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Tatsuya Tada ◽  
Tohru Miyoshi-Akiyama ◽  
Kayo Shimada ◽  
Masahiro Shimojima ◽  
Teruo Kirikae
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Pseudomonas Aeruginosa ◽  
Acquired Resistance ◽  
Amino Groups ◽  
Aminoglycoside Resistance ◽  
Class 1 Integron ◽  
Content Type ◽  
Class 1 ◽  
Layer Chromatography

ABSTRACTPseudomonas aeruginosaNCGM1588 has a novel chromosomal class 1 integron, In151, which includes theaac(6′)-Iajgene. The encoded protein, AAC(6′)-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6′)-Ia.Escherichia colitransformed with a plasmid containing theaac(6′)-Iajgene acquired resistance to all aminoglycosides tested except gentamicin. Of note,aac(6′)-Iajcontributed to the resistance to arbekacin. Thin-layer chromatography revealed that AAC(6′)-Iaj acetylated all aminoglycosides tested except gentamicin. These findings indicated that AAC(6′)-Iaj is a functional acetyltransferase that modifies the amino groups at the 6′ positions of aminoglycosides and contributes to aminoglycoside resistance ofP. aeruginosaNCGM1588, including arbekacin.

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