scholarly journals Integration of a Transposon Tn1-Encoded Inhibitor-Resistant β-Lactamase Gene, blaTEM-67 from Proteus mirabilis, into the Escherichia coli Chromosome

2003 ◽  
Vol 47 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Thierry Naas ◽  
Marie Zerbib ◽  
Delphine Girlich ◽  
Patrice Nordmann
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Proteus Mirabilis ◽  
Care Facility ◽  
Amino Acid Identity ◽  
Biochemical Properties ◽  
Leader Peptide ◽  
Term Care ◽  
Long Term Care Facility

ABSTRACT Proteus mirabilis NEL-1 was isolated from a urine sample of a patient hospitalized in a long-term care facility. Strain NEL-1 produced a β-lactamase with a pI of 5.2 conferring resistance to amoxicillin and amoxicillin-clavulanic acid. Sequencing of a PCR amplicon by using TEM-specific primers revealed a novel bla TEM gene, bla TEM-67. TEM-67 was an IRT-1-like TEM derivative related to TEM-65 (Lys39, Cys244) with an additional Leu21Ile amino acid substitution in the leader peptide. The biochemical properties of TEM-67 were equivalent to those described for TEM-65. Analysis of sequences surrounding bla TEM-67 revealed that it was located on a transposon, Tn1, which itself was located on a 48-kb non-self-transferable plasmid, pANG-1. Electroporation of plasmid pANG-1 into Escherichia coli DH10B resulted in the integration of bla TEM-67 into the chromosome, whereas it remained episomal in the P. mirabilis CIP103181 reference strain. Further characterization of pANG-1 revealed the presence of two identical sequences on both sides of Tn1 that contained an IS26 insertion sequence followed by a novel colicin gene, colZ, which had 20% amino acid identity with other colicin genes. The characterization of this novel TEM derivative provides further evidence for the large diversity of plasmid-encoded β-lactamases produced in P. mirabilis and for their spread to other enterobacterial species through transposable-element-mediated events.

scholarly journals Characterization of blaCTX-M-27/F1:A2:B20 Plasmids Harbored by Escherichia coli Sequence Type 131 Sublineage C1/H30R Isolates Spreading among Elderly Japanese in Nonacute-Care Settings

2020 ◽  
Vol 64 (5) ◽  
Author(s):  
Nao Matsuo ◽  
Rina Nonogaki ◽  
Michiko Hayashi ◽  
Jun-ichi Wachino ◽  
Masahiro Suzuki ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Care Facility ◽  
Sequence Type ◽  
Health Concern ◽  
Term Care ◽  
Content Type ◽  
Long Term Care Facility ◽  
Elderly Japanese

ABSTRACT We characterized 29 blaCTX-M-27-harboring plasmids of Escherichia coli sequence type 131 (ST131) sublineage C1/H30R isolates from healthy individuals and long-term-care facility (LTCF) residents. Most (27/29) plasmids were of the FIA, FIB, and FII multireplicon type with the same plasmid multilocus sequence typing (pMLST). Several plasmids (7/23) from LTCF residents harbored only blaCTX-M-27 as the resistance gene; however, their fundamental structures were very similar to those of previously isolated blaCTX-M-27/F1:A2:B20 plasmids, suggesting their prevalence as a newly arising public health concern.

Heterologous expression and characterization of l-amino acid deaminases from Proteus mirabilis in Escherichia coli

2008 ◽  
Vol 136 ◽  
pp. S300 ◽  
Author(s):  
Jin-Oh Baek ◽  
Jeong-Woo Seo ◽  
Ohsuk Kwon ◽  
Su-Il Seong ◽  
Ik-Hwan Kim ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Heterologous Expression ◽  
Proteus Mirabilis

Emergence of multidrug-resistant Proteus mirabilis in a long-term care facility in Croatia

2016 ◽  
Vol 128 (11-12) ◽  
pp. 404-413 ◽  
Author(s):  
Branka Bedenić ◽  
Nataša Firis ◽  
Vesna Elveđi-Gašparović ◽  
Marija Krilanović ◽  
Krešimir Matanović ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Long Term Care ◽  
Care Facility ◽  
Multidrug Resistant ◽  
Term Care ◽  
Term Care Facility ◽  
Long Term Care Facility

scholarly journals Prolonged clonal spreading and dynamic changes in antimicrobial resistance of Escherichia coli ST68 among patients who stayed in a respiratory care ward

2014 ◽  
Vol 63 (11) ◽  
pp. 1531-1541 ◽  
Author(s):  
Chih-Ming Chen ◽  
Se-Chin Ke ◽  
Chia-Ru Li ◽  
Chien-Shun Chiou ◽  
Chao-Chin Chang
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Care Facility ◽  
Respiratory Care ◽  
Term Care ◽  
Long Term Care Facility ◽  
E Coli ◽  
Antimicrobial Resistance Genes ◽  
Care Ward

From 2007 to 2009, we collected a total of 83 bacteraemic isolates of Escherichia coli with reduced susceptibility or resistance to third-generation cephalosporins (TGCs). Isolates were genotyped by PFGE and multilocus sequence typing (MLST). The PFGE patterns revealed two highly correlated clusters (cluster E: nine isolates; cluster G: 22 isolates) associated with this prolonged clonal spreading. Compared with cluster E isolates, cluster G isolates were significantly more likely to harbour aac(6')-Ib-cr (P<0.05), and most of these isolates were isolated during a later year than cluster E isolates (P<0.05). By MLST analysis, 94 % of cluster E and G isolates (29/31) were ST68. Although no time or space clustering could be identified by the conventional hospital-acquired infection monitoring system, E. coli cases caused by cluster E and G isolates were significantly associated with having stayed in our hospital’s respiratory care ward (P<0.05). Isolates obtained from patients who had stayed in the respiratory care ward had a significantly higher rate of aac(6')-Ib-cr and bla CTX-M-14 positivity, and were more likely to belong to ST68/S68-like (all P<0.05). To our knowledge, this is the first report of prolonged clonal spreading caused by E. coli ST68 associated with a stay in a long-term care facility. Using epidemiological investigations and PFGE and MLST analyses, we have identified long-term clonal spreading caused by E. coli ST68, with extra antimicrobial-resistance genes possibly acquired during the prolonged spreading period.

scholarly journals Close proximity interactions support transmission of ESBL-K. pneumoniae but not ESBL-E. coli in healthcare settings

2018 ◽  
Author(s):  
Audrey Duval ◽  
Thomas Obadia ◽  
Pierre-Yves Boëlle ◽  
Eric Fleury ◽  
Jean-Louis Herrmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Infection Control ◽  
Long Term Care ◽  
Care Facility ◽  
Term Care ◽  
Term Care Facility ◽  
Long Term Care Facility ◽  
Close Proximity

AbstractAntibiotic-resistance of hospital-acquired infections is a major public health issue. The worldwide emergence and diffusion of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae, including Escherichia coli (ESBL-EC) and Klebsiella pneumoniae (ESBL-KP), is of particular concern. Preventing their nosocomial spread requires understanding their transmission. Using Close Proximity Interactions (CPIs), measured by wearable sensors, and weekly ESBL-EC– and ESBL-KP–carriage data, we traced their possible transmission paths among 329 patients in a 200-bed long-term care facility over 4 months. Based on phenotypically defined resistance profiles to 12 antibiotics, new bacterial acquisitions were tracked. Extending a previously proposed statistical method, the CPI network’s ability to support observed incident colonization episodes of ESBL-EC and ESBL-KP was tested. Finally, mathematical modeling based on our findings assessed the effect of several infection-control measures. A potential infector was identified in the CPI network for 80% (16/20) of ESBL-KP acquisition episodes. The lengths of CPI paths between ESBL-KP incident cases and their potential infectors were shorter than predicted by chance (P = 0.02), indicating that CPI-network relationships were consistent with dissemination. Potential ESBL-EC infectors were identified for 54% (19/35) of the acquisitions, with longer-than-expected lengths of CPI paths. These contrasting results yielded differing impacts of infection control scenarios, with contact reduction interventions proving less effective for ESBL-EC than for ESBL-KP. These results highlight the widely variable transmission patterns among ESBL-producing Enterobacteriaceae species CPI networks supported ESBL-KP, but not ESBL-EC spread. These outcomes could help design more specific surveillance and control strategies to prevent in-hospital Enterobacteriaceae dissemination.Author summaryTracing extended-spectrum β-lactamase (ESBL) dissemination in hospitals is an important step in the fight against the spread of multi-drug resistant bacteria. Indeed, understanding ESBL spreading dynamics will help identify efficient control interventions. In the i-Bird study, patients and hospital staff from a French long-term care facility in France carried a wearable sensor to capture their interactions at less than 1.5 meters, every 30 seconds over a 4-month period. Every week, patients were also swabbed to detect carriage of ESBL-producing Enterobacteriaceae. Based on the analysis of these longitudinal data, this study shows that ESBL-producing Klebsiella pneumoniae (ESBL-KP) mostly spreads during close-proximity interactions between individuals, while this is not the case for ESBL-producing Escherichia coli (ESBL-EC), suggesting that ESBL-KP but not ESBL-EC may be controlled by contact reduction interventions.

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