scholarly journals Siderophore Production by Pseudomonas stutzeri under Aerobic and Anaerobic Conditions

2007 ◽  
Vol 73 (18) ◽  
pp. 5857-5864 ◽  
Author(s):  
Sofia A. Essén ◽  
Anna Johnsson ◽  
Dan Bylund ◽  
Karsten Pedersen ◽  
Ulla S. Lundström
Keyword(s):  
Mass Spectrometry ◽  
Hard Rock ◽  
Bacterial Culture ◽  
Pseudomonas Stutzeri ◽  
Siderophore Production ◽  
Anaerobic Conditions ◽  
Rock Laboratory ◽  
Molecular Masses ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The siderophore production of the facultative anaerobe Pseudomonas stutzeri, strain CCUG 36651, grown under both aerobic and anaerobic conditions, was investigated by liquid chromatography and mass spectrometry. The bacterial strain has been isolated at a 626-m depth at the Äspö Hard Rock Laboratory, where experiments concerning the geological disposal of nuclear waste are performed. In bacterial culture extracts, the iron in the siderophore complexes was replaced by gallium to facilitate siderophore identification by mass spectrometry. P. stutzeri was shown to produce ferrioxamine E (nocardamine) as the main siderophore together with ferrioxamine G and two cyclic ferrioxamines having molecular masses 14 and 28 atomic mass units lower than that of ferrioxamine E, suggested to be ferrioxamine D2 and ferrioxamine X1, respectively. In contrast, no siderophores were observed from anaerobically grown P. stutzeri. None of the siderophores produced by aerobically grown P. stutzeri were found in anaerobic natural water samples from the Äspö Hard Rock Laboratory.

Perfluorooctane sulfonate - a quite mobile anionic anthropogenic surfactant, ubiquitously found in the environment

2004 ◽  
Vol 50 (5) ◽  
pp. 235-242 ◽  
Author(s):  
R.J.W. Meesters ◽  
H.Fr. Schröder
Keyword(s):  
Mass Spectrometry ◽  
Closed Loop ◽  
Perfluorooctane Sulfonate ◽  
Aerobic Treatment ◽  
Liquid Chromatography Mass Spectrometry ◽  
Anaerobic Conditions ◽  
Closed Loop Systems ◽  
Biochemical Degradation ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

The biochemical degradation of perfluorooctanesulfonate (PFOS) and perfluorooctanoic acid (PFOA) under aerobic and anaerobic conditions in closed-loop systems was monitored in laboratory scale. Adsorptive effects of these compounds to glass and polypropylene were also examined. Liquid chromatography/mass spectrometry (LC-MS) under negative electrospray (ESI(-)) conditions was applied for determination. Elimination of PFOS was observed under anaerobic conditions whereas aerobic treatment was not effective.

The Influences of Aerobic and Anaerobic Conditions on PHB and Glycerin Yields in the Process of Lignin Degradation by Pseudomonas stutzeri P156

2013 ◽  
Vol 634-638 ◽  
pp. 1170-1174 ◽  
Author(s):  
Zhi Wang ◽  
Wei Zi Qin ◽  
Shuai Shuai Bao ◽  
Xiong Chen ◽  
Feng Lian Zhang ◽  
...  
Keyword(s):  
Anaerobic Condition ◽  
Degradation Rate ◽  
Lignin Degradation ◽  
Lignin Content ◽  
Aerobic Condition ◽  
Pseudomonas Stutzeri ◽  
Anaerobic Conditions ◽  
The Cost ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

The effects of aerobic and anaerobic conditions on polyhydroxybutyrate (PHB) and glycerin yields during the course of lignin degradation were studied with Pseudomonas stutzeri P156. The lignin degradation rate was 22.5% and 15%, respectively, under aerobic and anaerobic condition. However, the gradation rate/biomass (YL/X) was nearly no differences that diversified from 0.9% to 3%. In addition, the maximal anaerobic yield of PHB and glycerin yield was 0.42 g/g and 56.3 ug/ml, which was 176.6% and 36.7% higher than that under aerobic condition, respectively. Interestingly to point out, anaerobic lignin degradation rate was decreased by 33.3% relative to the aerobic one, but lignin content did decreased by 15% at the cost of higher yields of PHB and glycerin, which suggested biosyntheses of PHB and glycerin could balance the redox potential under anaerobic condition, facilitate lignin degradation by Pseudomonas stutzeri P156, persistently, and meet the process demands of straw-based anaerobic methane fermentation.

Oxygen concentration affects frequency and range of transconjugants for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1

2020 ◽  
Vol 85 (4) ◽  
pp. 1005-1015
Author(s):  
Kentaro Ochi ◽  
Maho Tokuda ◽  
Kosuke Yanagiya ◽  
Chiho Suzuki-Minakuchi ◽  
Hideaki Nojiri ◽  
...  
Keyword(s):  
Oxygen Concentration ◽  
Anaerobic Condition ◽  
Environmental Samples ◽  
Aerobic Condition ◽  
Recipient Strain ◽  
Donor Strain ◽  
Anaerobic Conditions ◽  
Filter Mating ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

ABSTRACT The frequency of transconjugants were compared for the incompatibility (Inc) P-1 and P-7 plasmids pBP136 and pCAR1 under aerobic and anaerobic conditions. Filter mating assays were performed with one donor strain and one recipient strain using different donors of Pseudomonas and recipient strains, including Pseudomonas, Pantoea, and Buttiauxella. Under anaerobic condition, frequencies of transconjugants for both plasmids were 101-103-fold lower than those under aerobic condition regardless of whether aerobically or anaerobically grown donors and recipients were used. To compare the transconjugant ranges under aerobic and anaerobic conditions, conjugation was performed between the donor of pBP136 and recipient bacteria extracted from environmental samples. Several transconjugants were uniquely obtained from each aerobic or anaerobic condition. Our findings indicate that a plasmid can differently spread among bacteria depending on the oxygen concentrations of the environment.

PRODUCTION AND PROPERTIES OF 2,3-BUTANEDIOL: VII. FERMENTATION OF WHEAT BY AEROBACILLUS POLYMYXA UNDER AEROBIC AND ANAEROBIC CONDITIONS

1946 ◽  
Vol 24f (1) ◽  
pp. 1-11 ◽  
Author(s):  
G. A. Adams
Keyword(s):  
Carbon Dioxide ◽  
Anaerobic Fermentation ◽  
Anaerobic Conditions ◽  
Mechanical Agitation ◽  
Fermentation Time ◽  
Aerobic Conditions ◽  
Ethanol Formation ◽  
Time Required ◽  
Aerobic And Anaerobic ◽  
Aerobic And Anaerobic Conditions

Aeration by mechanical agitation of 15% wheat mash fermented by Aerobacillus polymyxa inhibited the formation of 2,3-butanediol and particularly of ethanol. Aeration of similar mashes by passage of finely dispersed air or oxygen at the rate of 333 ml. per minute per litre of mash increased the rate of formation and yield of 2,3-butanediol but inhibited ethanol formation. However, the over-all time required for the completion of fermentation was not shortened from the usual 72 to 96 hr. required for unaerated mashes. There was no evidence of a shift from fermentative to oxidative dissimilation. Under aerobic conditions, the final butanediol–ethanol ratio was approximately 3:1. Anaerobic conditions, as produced by the passage of nitrogen or hydrogen through the mash, increased the rate of formation of both butanediol and ethanol and shortened the fermentation time to about 48 hr. Under these conditions, the butanediol–ethanol ratio was reduced to about 1.3:1.0. Carbon dioxide gave a butanediol–ethanol ratio resembling that of anaerobic fermentation but did not reduce fermentation time.

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