scholarly journals Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

2006 ◽  
Vol 72 (12) ◽  
pp. 7886-7893 ◽  
Author(s):  
Ayalkibet Hundesa ◽  
Carlos Maluquer de Motes ◽  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Rosina Girones
Keyword(s):  
River Water ◽  
Water Samples ◽  
Environmental Samples ◽  
Fecal Contamination ◽  
Source Tracking ◽  
Animal Origin ◽  
Urban Sewage ◽  
River Water Samples ◽  
Negative Results ◽  
Wastewater Samples

ABSTRACT The Adenoviridae and Polyomaviridae families comprise a wide diversity of viruses which may be excreted for long periods in feces or urine. In this study, a preliminary analysis of the prevalence in the environment and the potential usefulness as source-tracking tools of human and animal adenoviruses and polyomaviruses has been developed. Molecular assays based on PCR specifically targeting human adenoviruses (HAdV), porcine adenoviruses (PAdV), bovine adenoviruses (BAdV), and bovine polyomaviruses (BPyV) were applied to environmental samples including urban sewage, slaughterhouse, and river water samples. PAdV and BPyV were detected in a very high percentage of samples potentially affected by either porcine or bovine fecal contamination, respectively. However, BAdV were detected in only one sample, showing a lower prevalence than BPyV in the wastewater samples analyzed. The 22 slaughterhouse samples with fecal contamination of animal origin showed negative results for the presence of HAdV. The river water samples analyzed were positive for the presence of both human and animal adenoviruses and polyomaviruses, indicating the existence of diverse sources of contamination. The identities of the viruses detected were confirmed by analyses of the amplified sequences. All BPyV isolates showed a 97% similarity in nucleotide sequences. This is the first time that PAdV5, BAdV6, and BPyV have been reported to occur in environmental samples. Human and porcine adenoviruses and human and bovine polyomaviruses are proposed as tools for evaluating the presence of viral contamination and for tracking the origin of fecal/urine contamination in environmental samples.

scholarly journals Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination

2004 ◽  
Vol 70 (3) ◽  
pp. 1448-1454 ◽  
Author(s):  
Carlos Maluquer de Motes ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Margarita Mart�n ◽  
Rosina Girones
Keyword(s):  
Nested Pcr ◽  
Fecal Contamination ◽  
Human Adenovirus ◽  
The Other ◽  
Animal Origin ◽  
Viral Contamination ◽  
Urban Sewage ◽  
Negative Results ◽  
Pcr Assays ◽  
Nested Pcr Assays

ABSTRACT In this study, a molecular procedure for the detection of adenoviruses of animal origin was developed to evaluate the level of excretion of these viruses by swine and cattle and to design a test to facilitate the tracing of specific sources of environmental viral contamination. Two sets of oligonucleotides were designed, one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. The specificity of the assays was assessed in 31 fecal samples and 12 sewage samples that were collected monthly during a 1-year period. The data also provided information on the environmental prevalence of animal adenoviruses. Porcine adenoviruses were detected in 17 of 24 (70%) pools of swine samples studied, with most isolates being closely related to serotype 3. Bovine adenoviruses were present in 6 of 8 (75%) pools studied, with strains belonging to the genera Mastadenovirus and Atadenovirus and being similar to bovine adenoviruses of types 2, 4, and 7. These sets of primers produced negative results in nested PCR assays when human adenovirus controls and urban-sewage samples were tested. Likewise, the sets of primers previously designed for detection of human adenovirus also produced negative results with animal adenoviruses. These results indicate the importance of further studies to evaluate the usefulness of these tests to trace the source of fecal contamination in water and food and for environmental studies.

scholarly journals First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

2020 ◽  
Author(s):  
Eiji Haramoto ◽  
Bikash Malla ◽  
Ocean Thakali ◽  
Masaaki Kitajima
Keyword(s):  
River Water ◽  
Water Samples ◽  
Extraction Method ◽  
Limit Of Detection ◽  
Rna Extraction ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Wastewater Sample ◽  
River Water Samples ◽  
Wastewater Samples

ABSTRACTWastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200–5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 103 copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 × 103–8.2 × 104 copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 × 102–2.5 × 103 copies/L) with a higher filtration volume of 5,000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.

scholarly journals Detection of Human Group A Rotavirus in Urban Sewage, Hospital Sewage and River Water Samples in Alborz Province Using ELISA Method

2018 ◽  
Vol 28 (2) ◽  
pp. 124-129
Author(s):  
Zahra Torfeh ◽  
Naser Harzandi ◽  
Mostafa Ghaderi ◽  
◽  
◽  
...  
Keyword(s):  
River Water ◽  
Water Samples ◽  
Human Group ◽  
Elisa Method ◽  
Group A Rotavirus ◽  
Urban Sewage ◽  
River Water Samples ◽  
Group A ◽  
Hospital Sewage

Ecotoxicological studies using modified ames bioassay

1996 ◽  
Vol 34 (7-8) ◽  
pp. 1-7 ◽  
Author(s):  
M. Filipic ◽  
M. J. Toman
Keyword(s):  
River Water ◽  
Water Samples ◽  
Mutagenic Activity ◽  
Municipal Waste ◽  
Waste Dump ◽  
River Water Samples ◽  
Modified Method ◽  
Mutagenicity Assay ◽  
Wastewater Samples ◽  
The Impact

Health and environmental risk assessment of river and wastewaters based on single chemicals is limited by the number of chemicals that can be identified and to those chemicals for which toxicity and mutagenicity data exist. In this study Salmonella/microsome mutagenicity assay was evaluated as a potentially useful biomonitoring system for river water and wastewater. Standard assay was modified to allow testing of up to 2.5 ml of nonconcentrated water sample and by introducing 90 minutes of preincubation. Mutagenic activity of nonconcentrated samples was compared to the mutagenic activity of XAD-2 extracts of the same samples. Eighteen river water samples, two wastewater samples and one sample of water leaking from municipal waste dump were tested. Mutagenicity was detected in three nonconcentrated river water samples, both nonconcentrated wastewater samples and in the sample of the water leaking from the municipal waste dump. The mutagenic profiles of nonconcentrated samples were different from the mutagenic profiles of the XAD-2 extracts. The modified method was sensitive enough to detect mutagens present in more polluted river water (class IV), in wastewaters and in water leaking from the municipal dump. For the assessment of the impact of the mutagenic river and wastewaters on the environment, calculations from the results of nonconcentrated samples should be used as it seems that calculations from the results of the extracts are leading to the underestimation of mutagenic potency. Mutagenic profiles of the extracts are a useful guide for further chemical and biological analysis to trace the sources of the mutagens and to introduce measures to reduce them.

Colorimetric determination of nitrite in clinical, food and environmental samples using microfluidic devices stamped in paper platforms

2015 ◽  
Vol 7 (17) ◽  
pp. 7311-7317 ◽  
Author(s):  
Thiago M. G. Cardoso ◽  
Paulo T. Garcia ◽  
Wendell K. T. Coltro
Keyword(s):  
River Water ◽  
Water Samples ◽  
Environmental Samples ◽  
Statistical Difference ◽  
Reference Method ◽  
Microfluidic Devices ◽  
Colorimetric Determination ◽  
River Water Samples ◽  
Paper Microfluidic

Paper microfluidic devices were explored to perform the colorimetric determination of nitrite in saliva, sausage, ham, preservative water and river water samples with no statistical difference from a reference method.

Fabrication of dendritic nanoporous gold via a two-step amperometric approach: Application for electrochemical detection of methyl parathion in river water samples

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122130
Author(s):  
Gilberto J. Silva Junior ◽  
Jéssica Soares Guimarães Selva ◽  
Anandhakumar Sukeri ◽  
Josué M. Gonçalves ◽  
Matias Regiart ◽  
...  
Keyword(s):  
River Water ◽  
Electrochemical Detection ◽  
Water Samples ◽  
Methyl Parathion ◽  
Nanoporous Gold ◽  
River Water Samples

Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples

2010 ◽  
Vol 25 (6) ◽  
pp. 1376-1381 ◽  
Author(s):  
Noelia A. Martínez ◽  
Rudolf J. Schneider ◽  
Germán A. Messina ◽  
Julio Raba
Keyword(s):  
River Water ◽  
Water Samples ◽  
Microfluidic System ◽  
River Water Samples ◽  
Paramagnetic Beads

scholarly journals Seasonal Variations in the Physicochemical Parameters of Ikpoba River Water Samples

2018 ◽  
Vol 4 (3) ◽  
Author(s):  
Akpe Azuka Romanus ◽  
Femi Imah Justus ◽  
Okwu Grace Ifeoma ◽  
Obiazi Helen
Keyword(s):  
River Water ◽  
Seasonal Variations ◽  
Water Samples ◽  
Physicochemical Parameters ◽  
River Water Samples

Spectrophotometry determination of Congo red in river water samples using nanosilver

2012 ◽  
Vol 94 (10) ◽  
pp. 1886-1892 ◽  
Author(s):  
R. Sahraei ◽  
A. Farmany ◽  
S.S. Mortazavi ◽  
H. Noorizadeh
Keyword(s):  
River Water ◽  
Water Samples ◽  
Congo Red ◽  
River Water Samples
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