scholarly journals Synergistic Inhibition of Microbial Sulfide Production by Combinations of the Metabolic Inhibitor Nitrite and Biocides

2006 ◽  
Vol 72 (12) ◽  
pp. 7897-7901 ◽  
Author(s):  
E. Anne Greene ◽  
Veronique Brunelle ◽  
Gary E. Jenneman ◽  
Gerrit Voordouw
Keyword(s):  
Inhibitory Concentration ◽  
Cost Effective ◽  
Metabolic Inhibitors ◽  
Effective Control ◽  
Metabolic Inhibitor ◽  
Specific Inhibition ◽  
Synergistic Inhibition ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Reducing Bacteria

ABSTRACT MICs of six broad-spectrum biocides and two specific metabolic inhibitors and fractional inhibitory concentration indexes (FICIs) for controlling a sulfide-producing consortium were determined. Nitrite was synergistic (FICI < 1) with all but one biocide due to its specific inhibition of dissimilatory sulfite reductase. Hence, combining nitrite with biocides allows more efficient and cost-effective control of sulfate-reducing bacteria.

scholarly journals Experimental study on the treatment of acid mine drainage by modified corncob fixed SRB sludge particles

RSC Advances ◽  
2019 ◽  
Vol 9 (33) ◽  
pp. 19016-19030 ◽  
Author(s):  
Yan-Rong Dong ◽  
Jun-Zhen Di ◽  
Ming-Xin Wang ◽  
Ya-Dong Ren
Keyword(s):  
Experimental Study ◽  
Acid Mine Drainage ◽  
Mine Drainage ◽  
Cost Effective ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Acid Mine ◽  
Effective System ◽  
Reducing Bacteria

A cost-effective system for acid mine drainage removal was developed with the key role of alkaline H2O2 modified corncob and sulfate reducing bacteria.

scholarly journals Phylogeography of Sulfate-Reducing Bacteria among Disturbed Sediments, Disclosed by Analysis of the Dissimilatory Sulfite Reductase Genes (dsrAB)

2005 ◽  
Vol 71 (2) ◽  
pp. 1004-1011 ◽  
Author(s):  
J. R. Pérez-Jiménez ◽  
L. J. Kerkhof
Keyword(s):  
New York ◽  
Distribution Patterns ◽  
Sulfate Reducing Bacteria ◽  
Sulfite Reductase ◽  
Geographic Proximity ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Polluted Sites ◽  
Eukaryotic Organisms ◽  
Reducing Bacteria

ABSTRACT Sediment samples were collected worldwide from 16 locations on four continents (in New York, California, New Jersey, Virginia, Puerto Rico, Venezuela, Italy, Latvia, and South Korea) to assess the extent of the diversity and the distribution patterns of sulfate-reducing bacteria (SRB) in contaminated sediments. The SRB communities were examined by terminal restriction fragment (TRF) length polymorphism (TRFLP) analysis of the dissimilatory sulfite reductase genes (dsrAB) with NdeII digests. The fingerprints of dsrAB genes contained a total of 369 fluorescent TRFs, of which <20% were present in the GenBank database. The global sulfidogenic communities appeared to be significantly different among the anthropogenically impacted (petroleum-contaminated) sites, but nearly all were less diverse than pristine habitats, such as mangroves. A global SRB indicator species of petroleum pollution was not identified. However, several dsrAB gene sequences corresponding to hydrocarbon-degrading isolates or consortium members were detected in geographically widely separated polluted sites. Finally, a cluster analysis of the TRFLP fingerprints indicated that many SRB microbial communities were most similar on the basis of close geographic proximity (tens of kilometers). Yet, on larger scales (hundreds to thousands of kilometers) SRB communities could cluster with geographically widely separated sites and not necessarily with the site with the closest proximity. These data demonstrate that SRB populations do not adhere to a biogeographic distribution pattern similar to that of larger eukaryotic organisms, with the greatest species diversity radiating from the Indo-Pacific region. Rather, a patchy SRB distribution is encountered, implying an initially uniform SRB community that has differentiated over time.

scholarly journals Congruent Phylogenies of Most Common Small-Subunit rRNA and Dissimilatory Sulfite Reductase Gene Sequences Retrieved from Estuarine Sediments

2001 ◽  
Vol 67 (7) ◽  
pp. 3314-3318 ◽  
Author(s):  
Catherine Joulian ◽  
Niels B. Ramsing ◽  
Kjeld Ingvorsen
Keyword(s):  
Target Genes ◽  
Small Subunit ◽  
Estuarine Sediments ◽  
Sulfite Reductase ◽  
Small Subunit Rrna ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Phylogenetic Affiliation ◽  
Reductase Gene ◽  
Reducing Bacteria

ABSTRACT The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and producedDesulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.

scholarly journals Non-Sulfate-Reducing, Syntrophic Bacteria Affiliated with Desulfotomaculum Cluster I Are Widely Distributed in Methanogenic Environments

2006 ◽  
Vol 72 (3) ◽  
pp. 2080-2091 ◽  
Author(s):  
Hiroyuki Imachi ◽  
Yuji Sekiguchi ◽  
Yoichi Kamagata ◽  
Alexander Loy ◽  
Yan-Ling Qiu ◽  
...  
Keyword(s):  
Beta Subunits ◽  
Rrna Gene ◽  
The Novel ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Physiological Properties ◽  
Syntrophic Bacteria ◽  
Almost All ◽  
Classical Perception ◽  
Reducing Bacteria

ABSTRACT The classical perception of members of the gram-positive Desulfotomaculum cluster I as sulfate-reducing bacteria was recently challenged by the isolation of new representatives lacking the ability for anaerobic sulfate respiration. For example, the two described syntrophic propionate-oxidizing species of the genus Pelotomaculum form the novel Desulfotomaculum subcluster Ih. In the present study, we applied a polyphasic approach by using cultivation-independent and culturing techniques in order to further characterize the occurrence, abundance, and physiological properties of subcluster Ih bacteria in low-sulfate, methanogenic environments. 16S rRNA (gene)-based cloning, quantitative fluorescence in situ hybridization, and real-time PCR analyses showed that the subcluster Ih population composed a considerable part of the Desulfotomaculum cluster I community in almost all samples examined. Additionally, five propionate-degrading syntrophic enrichments of subcluster Ih bacteria were successfully established, from one of which the new strain MGP was isolated in coculture with a hydrogenotrophic methanogen. None of the cultures analyzed, including previously described Pelotomaculum species and strain MGP, consumed sulfite, sulfate, or organosulfonates. In accordance with these phenotypic observations, a PCR-based screening for dsrAB (key genes of the sulfate respiration pathway encoding the alpha and beta subunits of the dissimilatory sulfite reductase) of all enrichments/(co)cultures was negative with one exception. Surprisingly, strain MGP contained dsrAB, which were transcribed in the presence and absence of sulfate. Based on these and previous findings, we hypothesize that members of Desulfotomaculum subcluster Ih have recently adopted a syntrophic lifestyle to thrive in low-sulfate, methanogenic environments and thus have lost their ancestral ability for dissimilatory sulfate/sulfite reduction.

scholarly journals Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers

2006 ◽  
Vol 73 (2) ◽  
pp. 594-604 ◽  
Author(s):  
Shabir A. Dar ◽  
Li Yao ◽  
Udo van Dongen ◽  
J. Gijs Kuenen ◽  
Gerard Muyzer
Keyword(s):  
16S Rrna ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Bacterial Populations ◽  
Sulfate Reducing ◽  
Dna And Rna ◽  
Dissimilatory Sulfite Reductase ◽  
Pcr Products ◽  
Gradient Gel Electrophoresis ◽  
Reducing Bacteria

ABSTRACTHere we describe the diversity and activity of sulfate-reducing bacteria (SRB) in sulfidogenic bioreactors by using the simultaneous analysis of PCR products obtained from DNA and RNA of the 16S rRNA and dissimilatory sulfite reductase (dsrAB) genes. We subsequently analyzed the amplified gene fragments by using denaturing gradient gel electrophoresis (DGGE). We observed fewer bands in the RNA-based DGGE profiles than in the DNA-based profiles, indicating marked differences in the populations present and in those that were metabolically active at the time of sampling. Comparative sequence analyses of the bands obtained from rRNA anddsrBDGGE profiles were congruent, revealing the same SRB populations. Bioreactors that received either ethanol or isopropanol as an energy source showed the presence of SRB affiliated withDesulfobulbus rhabdoformisand/orDesulfovibrio sulfodismutans, as well as SRB related to the acetate-oxidizingDesulfobacca acetoxidans. The reactor that received wastewater containing a diverse mixture of organic compounds showed the presence of nutritionally versatile SRB affiliated withDesulfosarcina variabilisand another acetate-oxidizing SRB, affiliated withDesulfoarculus baarsii. In addition to DGGE analysis, we performed whole-cell hybridization with fluorescently labeled oligonucleotide probes to estimate the relative abundances of the dominant sulfate-reducing bacterial populations.Desulfobacca acetoxidans-like populations were most dominant (50 to 60%) relative to the total SRB communities, followed byDesulfovibrio-like populations (30 to 40%), andDesulfobulbus-like populations (15 to 20%). This study is the first to identify metabolically active SRB in sulfidogenic bioreactors by using the functional genedsrABas a molecular marker. The same approach can also be used to infer the ecological role of coexisting SRB in other habitats.

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