scholarly journals Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia

2010 ◽  
Vol 76 (6) ◽  
pp. 1996-2001 ◽  
Author(s):  
Chris A. Whitehouse ◽  
Carson Baldwin ◽  
Rangarajan Sampath ◽  
Lawrence B. Blyn ◽  
Rachael Melton ◽  
...  

ABSTRACT The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.

Author(s):  
Natalia Andrulionis ◽  
Natalia Andrulionis ◽  
Ivan Zavialov ◽  
Ivan Zavialov ◽  
Elena Kovaleva ◽  
...  

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.


RSC Advances ◽  
2018 ◽  
Vol 8 (31) ◽  
pp. 17293-17299 ◽  
Author(s):  
Wei Kou ◽  
Hua Zhang ◽  
Aisha Bibi ◽  
Mufang Ke ◽  
Jing Han ◽  
...  

A simple, fast and high-sensitivity method for quantification of fluoroquinolones in environmental water samples using MIPs-iEESI-MS.


2015 ◽  
Vol 12 (7) ◽  
pp. 7430-7443 ◽  
Author(s):  
Stefan Panaiotov ◽  
Ivan Simeonovski ◽  
Victoria Levterova ◽  
Ventzislav Karamfilov ◽  
Nadia Brankova ◽  
...  

Author(s):  
Taras Vintskovskyi

In Soviet historical science during 1960s-1980s a traditional stereotype of perception of the cruiser “Almaz” as “Southern Aurora” was formed, which had to symbolize similar tendencies of the revolutionary progress in 1917- 1918 in Baltic and the Black Sea Fleets. The role of the steamship crew in events of the Russian and the Ukrainian revolutions in a limited period of time is analyzed in the article. In January 1918, the Bolshevist armed insurrection took place in Odesa, active participation in the preparation of which was played by the part of sailors and officers of the cruiser “Almaz”. During the existence of the Odesa Soviet republic, new authorities used the practice of the “red” terror, the symbol of which was “Almaz”. As the result of the detention and physical liquidation of people of various categories on the cruiser and at moorings of other warships, thus spreading a variety of rumours, authorities of local self-government attempted to take control of the situation in order to make facts of self-trial impossible. But judicial and investigational institutions were able to investigate the circumstances of the detention of prisoners and their subsequent fate fully only after the restoration of the Ukrainian National Republic governance in Odesa. Their conclusions dispel the heroic image of the cruiser created by Soviet historiography.


Author(s):  
Natalia Andrulionis ◽  
Natalia Andrulionis ◽  
Ivan Zavialov ◽  
Ivan Zavialov ◽  
Elena Kovaleva ◽  
...  

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.


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