Two-Year Monitoring of Water Samples from Dam of Iskar and the Black Sea, Bulgaria, by Molecular Analysis: Focus on Mycobacterium spp.

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.

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Identification of Pathogenic Vibrio Species by Multilocus PCR-Electrospray Ionization Mass Spectrometry and Its Application to Aquatic Environments of the Former Soviet Republic of Georgia

Applied and Environmental Microbiology ◽

10.1128/aem.01919-09 ◽

2010 ◽

Vol 76 (6) ◽

pp. 1996-2001 ◽

Cited By ~ 15

Author(s):

Chris A. Whitehouse ◽

Carson Baldwin ◽

Rangarajan Sampath ◽

Lawrence B. Blyn ◽

Rachael Melton ◽

...

Keyword(s):

Mass Spectrometry ◽

Black Sea ◽

Water Samples ◽

The Black Sea ◽

Aquatic Environments ◽

Ionization Mass ◽

Freshwater Lakes ◽

Total Water ◽

Soviet Republic ◽

Pathogenic Vibrio

ABSTRACT The Ibis T5000 is a novel diagnostic platform that couples PCR and mass spectrometry. In this study, we developed an assay that can identify all known pathogenic Vibrio species and field-tested it using natural water samples from both freshwater lakes and the Georgian coastal zone of the Black Sea. Of the 278 total water samples screened, 9 different Vibrio species were detected, 114 (41%) samples were positive for V. cholerae, and 5 (0.8%) samples were positive for the cholera toxin A gene (ctxA). All ctxA-positive samples were from two freshwater lakes, and no ctxA-positive samples from any of the Black Sea sites were detected.

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SITE-SPECIFIC EQUATIONS OF STATE FOR COASTAL SEA AREAS AND INLAND WATER BODIES

Proceedings of International Conference "Managinag risks to coastal regions and communities in a changinag world" (EMECS'11 - SeaCoasts XXVI) ◽

10.21610/conferencearticle_589b5bebd4a21 ◽

2017 ◽

Author(s):

Natalia Andrulionis ◽

Ivan Zavialov ◽

Elena Kovaleva ◽

...

Keyword(s):

Equation Of State ◽

Black Sea ◽

Water Samples ◽

Equations Of State ◽

Sea Water ◽

Ionic Composition ◽

Water Bodies ◽

Aral Sea ◽

The Black Sea ◽

Coastal Sea

This article presents a new method of laboratory density determination and construction equations of state for marine waters with various ionic compositions and salinities was developed. The validation of the method was performed using the Ocean Standard Seawater and the UNESCO thermodynamic equation of state (EOS-80). Density measurements of water samples from the Aral Sea, the Black Sea and the Issyk-Kul Lake were performed using a high-precision laboratory density meter. The obtained results were compared with the density values calculated for the considered water samples by the EOS-80 equation. It was shown that difference in ionic composition between Standard Seawater and the considered water bodies results in significant inaccuracies in determination of water density using the EOS-80 equation. Basing on the laboratory measurements of density under various salinity and temperature values we constructed a new equation of state for the Aral Sea and the Black Sea water samples and estimated errors for their coefficients.

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Survey and first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea, Turkey

Journal of Water and Health ◽

10.2166/wh.2021.250 ◽

2021 ◽

Author(s):

Ulku Karaman ◽

Zeynep Koloren ◽

Panagiotis Karanis

Keyword(s):

Water Resources ◽

Black Sea ◽

Water Samples ◽

Water Sources ◽

Spring Water ◽

The Black Sea ◽

First Report ◽

Natural Spring Water ◽

Acanthamoeba Species ◽

Natural Spring

Abstract Infection with Acanthamoeba spp. may result in granulomatous amoebic encephalitis and Acanthamoeba keratitis. Water is an important habitat where Acanthamoeba species thrive. Therefore, studying the occurrence of this free-living amoeba in water sources will help understand the infection dynamics. The aim of the study was to survey and report on the presence of Acanthamoeba spp. in water resources from the Ordu and Giresun provinces in Black Sea. Acanthamoeba spp. was found in 1/17 natural spring water samples from Ordu and in 2/18 from Giresun. Acanthamoeba species were not detected in any of the investigated tap water samples. Sequencing of the (SSU) rDNA gene resulted in the identification of haplotype I (Acanthamoeba genotype: KJ094684). T4 (8.6%) was the only isolated genotype in both Ordu and Giresun provinces. This is the first report of Acanthamoeba T4 genotype in natural spring water resources in the Black Sea. The occurrence of Acanthamoeba species in natural spring water sources should be considered as a potential risk for human infection, especially to high-risk populations.

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Acanthamoeba spp. in river water samples from the Black Sea region, Turkey

Journal of Water and Health ◽

10.2166/wh.2020.170 ◽

2020 ◽

Vol 18 (2) ◽

pp. 186-199 ◽

Cited By ~ 1

Author(s):

İlknur Koyun ◽

Zeynep Kolören ◽

Ülkü Karaman ◽

Amalia Tsiami ◽

Panagiotis Karanis

Keyword(s):

Black Sea ◽

Water Samples ◽

Tap Water ◽

Acanthamoeba Keratitis ◽

The Black Sea ◽

Free Living ◽

Black Sea Region ◽

River Waters ◽

Free Living Amoeba ◽

Acanthamoeba Species

Abstract The present study aims to investigate the occurrence of free living amoeba (FLA) in water resources (rivers and tap water) in Samsun in the Black Sea. The presence of Acanthamoeba spp. was confirmed in 98 of 192 water samples collected from 32 sites of Samsun province (Samsun centre, Terme, Carsamba, Tekkekoy, Bafra) by PCR. Acanthamoeba spp. were found in 15/36 river samples from Samsun, in 58/90 from Terme, in 12/30 from Carsamba, in 7/18 from Tekkekoy and in 6/18 from Bafra. No Acanthamoeba species were detected in tap water samples. The highest rate in river waters contaminated with Acanthamoeba species was in Terme followed by Samsun centre (41.7%), Carsamba (40%), Tekkekoy (38.9%) and Bafra districts (33.3%), respectively. The result of the subsequent sequence analysis showed Haplotype I (A. triangularis) in 5%, Haplotype II (A. polyphaga) in 29.6%, Haplotype III (Acanthamoeba spp.) in 62% and Haplotype IV (A. lenticulata) in 3%. The most common genotype was Acanthamoeba T4 (Acanthamoeba spp., A. polyphaga, A. triangularis) and T5 genotype was also found in 3%. The T4 genotype is the most common genotype associated with Acanthamoeba keratitis (AK) worldwide; therefore, humans and animals living in the area are at risk after contact with such waters.

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The polycyclic aromatic hydrocarbons in the water of the Black Sea studied during the research cruise no. 114 aboard the r/v “Professor Vodyanitsky” in 2020

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/937/2/022066 ◽

2021 ◽

Vol 937 (2) ◽

pp. 022066

Author(s):

T O Barabashin ◽

L F Pavlenko ◽

G V Skrypnik ◽

T L Klimenko

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Black Sea ◽

Aromatic Hydrocarbons ◽

Water Samples ◽

Bottom Layer ◽

The Black Sea ◽

Aromatic Rings ◽

Crimean Peninsula ◽

Polycyclic Aromatic ◽

Almost All

Abstract The content and composition of polycyclic aromatic hydrocarbons have been studied in the water column (surface layer, thermocline layer, at a depth of 500 m and the bottom layer) of the deep-water areas of the Black Sea near the Crimean Peninsula and the Caucasian coast, and the results are presented. The identified PAHs included naphthalene, 2-methylnaphthalene, phenanthrene, anthracene, fluoranthene, pyrene, triphenylene, chrysene, benz (b) fluoranthene, benz(k)fluoranthene, benz(a)pyrene, dibenz(a,h)anthracene, benzo(g,h,i)perylene. The concentrations of the total PAHs detected near the capes Sarych and Chauda were the highest, 532 and 593 ng/L, respectively. The distribution of PAHs over the horizons did not have a clear pattern. The composition of PAHs was dominated by 2-nuclear compounds, namely, naphthalene and 2-methylnaphthalene, their share on average accounted for 63 % of the total of the PAHs identified. The most dangerous nuclear PAHs containing 5 and 6 aromatic rings (benzo(b)fluoranthene, benz(k)fluoranthene, benzo(a)pyrene, dibenz(a,h)anthracene) accounted for less than 0.5 % in all the analyzed water samples. According to the calculated indices of technogenicity and pyrogenicity, PAHs of naphthydogenic origin predominated in almost all analyzed water samples. PAHs of technogenic oil origin prevailed in the water samples taken in the zone of shipping lanes to the port of Novorossiysk.

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