scholarly journals Enhancement of UV Light Sensitivity of a Vibrio parahaemolyticus O3:K6 Pandemic Strain Due to Natural Lysogenization by a Telomeric Phage

2009 ◽  
Vol 75 (6) ◽  
pp. 1697-1702 ◽  
Author(s):  
Beatriz Zabala ◽  
Katherine García ◽  
Romilio T. Espejo
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Uv Light ◽  
Bacterial Pathogen ◽  
Light Sensitivity ◽  
Alkaline Extraction ◽  
Clonal Population ◽  
Pandemic Strain ◽  
Clonal Strain ◽  
Similar Restriction

ABSTRACT The Vibrio parahaemolyticus O3:K6 pandemic clonal strain was first observed in southern Chile in 2004 and has since caused approximately 8,000 seafood-related diarrhea cases in this region. The massive proliferation of the original clonal population offers a unique opportunity to study the evolution of a bacterial pathogen in its natural environment by detection and characterization of emerging bacterial variants. Here, we describe a group of pandemic variants characterized by the presence of a 42-kb extrachromosomal DNA that can be recovered by alkaline extraction. Upon treatment with mitomycin C, these variants lyse with production of a myovirus containing DNA of equal size to the plasmid but which cannot be recovered by alkaline extraction. Plasmid and phage DNAs show similar restriction patterns corresponding to enzyme sites in a circular permutation. Sequenced regions showed 81 to 99% nucleotide similarity to bacteriophage VHML of Vibrio harveyi. Altogether these observations indicate that the 42-kb plasmid corresponds to a prophage, consisting of a linear DNA with terminal hairpins of a telomeric temperate phage with a linear genome. Bacteria containing the prophage were 7 to 15 times more sensitive to UV radiation, likely due to phage induction by UV irradiation as plasmid curing restored the original sensitivity. The enhanced UV sensitivity could have a significant role in reducing the survival and propagation capability of the V. parahaemolyticus pandemic strain in the ocean.

scholarly journals Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (O3[ratio ]K6 and O4[ratio ]K68) in Thailand and India

2000 ◽  
Vol 125 (1) ◽  
pp. 17-25 ◽  
Author(s):  
N. R. CHOWDHURY ◽  
S. CHAKRABORTY ◽  
B. EAMPOKALAP ◽  
W. CHAICUMPA ◽  
M. CHONGSA-NGUAN ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Time Frame ◽  
Molecular Techniques ◽  
Dna Fingerprint ◽  
Gene Characterization ◽  
Geographical Regions ◽  
Similar Restriction ◽  
First Time ◽  
Close Genetic Relationship

Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4[ratio ]K68 serovar for the first time in March 1998 alongside the continued predominant incidence of the O3[ratio ]K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3[ratio ]K6 and O4[ratio ]K68 strains and for comparison, non-O3[ratio ]K6 and non-O4[ratio ]K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3[ratio ]K6 and O4[ratio ]K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of strains yielded nearly similar restriction fragment length polymorphism (RFLP) patterns for the O3[ratio ]K6 and O4[ratio ]K68 isolates from Calcutta and Thailand. Phylogenetic analysis of the NotI RFLP showed that the O3[ratio ]K6 and O4[ratio ]K68 strains formed a cluster with 78–91% similarity thus indicating close genetic relationship between the two different serovars isolated during the same time-frame but from widely separated geographical regions. The non-O3[ratio ]K6 and non-O4[ratio ]K68, in contrast, showed different ribotype, AP-PCR and PFGE patterns.

scholarly journals Development and characterization of probiotic mucilage based edible films for the preservation of fruits and vegetables

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Seyed Mohammad Davachi ◽  
Neethu Pottackal ◽  
Hooman Torabi ◽  
Alireza Abbaspourrad
Keyword(s):  
Fruits And Vegetables ◽  
Uv Light ◽  
Barrier Properties ◽  
Edible Films ◽  
Food Preservation ◽  
Elongation At Break ◽  
The Public ◽  
Medical Benefits ◽  
Seed Mucilage

AbstractThere is growing interest among the public and scientific community toward the use of probiotics to potentially restore the composition of the gut microbiome. With the aim of preparing eco-friendly probiotic edible films, we explored the addition of probiotics to the seed mucilage films of quince, flax, and basil. These mucilages are natural and compatible blends of different polysaccharides that have demonstrated medical benefits. All three seed mucilage films exhibited high moisture retention regardless of the presence of probiotics, which is needed to help preserve the moisture/freshness of food. Films from flax and quince mucilage were found to be more thermally stable and mechanically robust with higher elastic moduli and elongation at break than basil mucilage films. These films effectively protected fruits against UV light, maintaining the probiotics viability and inactivation rate during storage. Coated fruits and vegetables retained their freshness longer than uncoated produce, while quince-based probiotic films showed the best mechanical, physical, morphological and bacterial viability. This is the first report of the development, characterization and production of 100% natural mucilage-based probiotic edible coatings with enhanced barrier properties for food preservation applications containing probiotics.

scholarly journals Characterization of a novel endolysin from bacteriophage infecting Vibrio parahaemolyticus, vB_VpaP_KF2

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Jeong-A Lim ◽  
Nari Lee ◽  
Hyang-Sook Chun ◽  
Hyun-Joo Chang
Keyword(s):  
Vibrio Parahaemolyticus

scholarly journals First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75425 ◽  
Author(s):  
Xie Zhenyu ◽  
Ke Shaowen ◽  
Hu Chaoqun ◽  
Zhu Zhixiong ◽  
Wang Shifeng ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Bacterial Pathogen ◽  
Vibrio Alginolyticus ◽  
The South China Sea ◽  
The South ◽  
White Syndrome ◽  
China Sea

scholarly journals Genome diversification within a clonal population of pandemic Vibrio parahaemolyticus seems to depend on the life circumstances of each individual bacteria

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
pp. 176 ◽  
Author(s):  
David E Loyola ◽  
Cristell Navarro ◽  
Paulina Uribe ◽  
Katherine García ◽  
Claudia Mella ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Clonal Population ◽  
Life Circumstances

Growth and characterization of ammonium nickel-cobalt sulfate Tutton’s salt for UV light applications

2018 ◽  
Vol 487 ◽  
pp. 104-115 ◽  
Author(s):  
Santunu Ghosh ◽  
Michelle Oliveira ◽  
Tiago S. Pacheco ◽  
Genivaldo J. Perpétuo ◽  
Carlos J. Franco
Keyword(s):  
Uv Light ◽  
Cobalt Sulfate ◽  
Nickel Cobalt

Mechanical Characterization of UV Photopolymerized PMMA with Different Photo-Initiator Concentration

2021 ◽  
Vol 903 ◽  
pp. 11-16
Author(s):  
M.A. Manjunath ◽  
K. Naveen ◽  
Prakash Vinod ◽  
N. Balashanmugam ◽  
M.R. Shankar
Keyword(s):  
Mechanical Properties ◽  
Light Source ◽  
Polymethyl Methacrylate ◽  
Biomedical Applications ◽  
Mechanical Characterization ◽  
Uv Light ◽  
Curing Time ◽  
Uv Led ◽  
Pmma Resin

Polymethyl methacrylate (PMMA) is one among few known photo-polymeric resin useful in lithography for fabricating structures having better mechanical properties to meet the requirement in electronics and biomedical applications. This study explores the effect of Photo Initiator (PI) concentration and also curing time on strength and hardness of Polymethyl methacrylate (PMMA) obtained by UV photopolymerization of Methyl methacrylate (MMA) monomer. The UV LED light source operating at the wavelength of 364 nm is used with Benzoin Ethyl Ether (BEE) as photo initiator. The curing of PMMA resin is supported with peltier cooling device placed at the bottom of the UV light source. The characterisation study of UV photo cured PMMA is analysed through nano indenter (Agilent Technologies-G200). The current work investigates the influence of PI concentration and curing time in achieving maximum mechanical properties for UV photopolymerized PMMA.

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