scholarly journals Ex VivoApplication of Secreted Metabolites Produced by Soil-Inhabiting Bacillus spp. Efficiently Controls Foliar Diseases Caused by Alternaria spp.

2015 ◽  
Vol 82 (2) ◽  
pp. 478-490 ◽  
Author(s):  
Gul Shad Ali ◽  
Ashraf S. A. El-Sayed ◽  
Jaimin S. Patel ◽  
Kari B. Green ◽  
Mohammad Ali ◽  
...  
Keyword(s):  
Biological Control ◽  
Plant Pathogens ◽  
Foliar Application ◽  
Control Plant ◽  
Complex Mixture ◽  
Biological Control Agents ◽  
In Planta ◽  
Content Type ◽  
Foliar Diseases ◽  
Culture Filtrates

ABSTRACTBacterial biological control agents (BCAs) are largely used as live products to control plant pathogens. However, due to variable environmental and ecological factors, live BCAs usually fail to produce desirable results against foliar pathogens. In this study, we investigated the potential of cell-free culture filtrates of 12 different bacterial BCAs isolated from flower beds for controlling foliar diseases caused byAlternariaspp.In vitrostudies showed that culture filtrates from two isolates belonging toBacillus subtilisandBacillus amyloliquefaciensdisplayed strong efficacy and potencies againstAlternariaspp. The antimicrobial activity of the culture filtrate of these two biological control agents was effective over a wider range of pH (3.0 to 9.0) and was not affected by autoclaving or proteolysis. Comparative liquid chromatography-mass spectrometry (LC-MS) analyses showed that a complex mixture of cyclic lipopeptides, primarily of the fengycin A and fengycin B families, was significantly higher in these two BCAs than inactiveBacillusspp. Interaction studies with mixtures of culture filtrates of these two species revealed additive activity, suggesting that they produce similar products, which was confirmed by LC-tandem MS analyses. Inin plantapre- and postinoculation trials, foliar application of culture filtrates ofB. subtilisreduced lesion sizes and lesion frequencies caused byAlternaria alternataby 68 to 81%. Taken together, our studies suggest that instead of live bacteria, culture filtrates ofB. subtilisandB. amyloliquefacienscan be applied either individually or in combination for controlling foliar diseases caused byAlternariaspecies.

scholarly journals Biocontrol of Phytopathogens under Aquaponics Systems

Water ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 2061 ◽  
Author(s):  
Tomás Rivas-García ◽  
Ramsés Ramón González-Estrada ◽  
Roberto Gregorio Chiquito-Contreras ◽  
Juan José Reyes-Pérez ◽  
Uriel González-Salas ◽  
...  
Keyword(s):  
Biological Control ◽  
Food Production ◽  
Plant Pathogens ◽  
Control Plant ◽  
Plant Diseases ◽  
Biological Control Agents ◽  
Economic Resource ◽  
Systems Research ◽  
Chemical Inputs ◽  
Potential Use

Aquaponics is an alternative method of food production that confers advantages of biological and economic resource preservations. Nonetheless, one of the main difficulties related to aquaponics systems could be the outbreak and dissemination of pathogens. Conventional treatments need to be administrated carefully because they could be harmful to human, fish, plants and beneficial microorganisms. Aquaponics practitioners are relatively helpless against plant diseases when they occur, especially in the case of root pathogens. Biological control agents (BCAs) may be an effective alternative to chemical inputs for dealing with pathogens of plants under aquaponics systems. Research of BCAs on aquaponics systems is limited, but there are numerous publications on the use of BCAs to control plant pathogens under soilless systems which confirm its potential use on aquaponics systems. The present review summarized the principal plant pathogens, the conventional and alternative BCA treatments on aquaponics systems, while considering related research on aquaculture and soilless systems (i.e., hydroponic) for its applicability to aquaponics and future perspectives related to biological control. Finally, we emphasized the case that aquaponics systems provide relatively untapped potential for research on plant biological control agents. Biological control has the potential to reduce the perturbation effects of conventional treatments on microbial communities, fish and plant physiology, and the whole function of the aquaponics system.

Endophytic Microorganisms as Biological Control Agents for Plant Pathogens: A Panacea for Sustainable Agriculture

2019 ◽  
pp. 1-20
Author(s):  
Charles Oluwaseun Adetunji ◽  
Deepak Kumar ◽  
Meenakshi Raina ◽  
Olawale Arogundade ◽  
Neera Bhalla Sarin
Keyword(s):  
Biological Control ◽  
Sustainable Agriculture ◽  
Plant Pathogens ◽  
Biological Control Agents ◽  
Endophytic Microorganisms

scholarly journals Draft Genome Sequence of Bacillus licheniformis Strain UASWS1606, a Plant Biostimulant for Agriculture

2020 ◽  
Vol 9 (37) ◽  
Author(s):  
Julien Crovadore ◽  
Bastien Cochard ◽  
Damien Grizard ◽  
Romain Chablais ◽  
Marine Baillarguet ◽  
...  
Keyword(s):  
Biological Control ◽  
Genome Sequence ◽  
Bacillus Licheniformis ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Biological Control Agents ◽  
Content Type ◽  
Agricultural Biostimulant

ABSTRACT Bacillus licheniformis is a well-known industrial bacterium. New strains show interesting properties of biostimulants and biological control agents for agriculture. Here, we report the draft genome sequence, obtained with an Illumina MiniSeq system, of strain UASWS1606 of the bacterium Bacillus licheniformis, which is being developed as an agricultural biostimulant.

Infection of plant-parasitic nematodes by nematophagous fungi – a review of the application of molecular biology to understand infection processes and to improve biological control

Nematology ◽  
2004 ◽  
Vol 6 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Oliver Morton ◽  
Penny Hirsch ◽  
Brian Kerry
Keyword(s):  
Biological Control ◽  
Molecular Biology ◽  
Control Plant ◽  
Nematophagous Fungi ◽  
Parasitic Nematodes ◽  
Biological Control Agents ◽  
Plant Parasitic Nematodes ◽  
Parasitic Fungi ◽  
Infection Processes ◽  
Plant Parasitic

AbstractEnvironmental concerns over conventional nematicides have led to increasing interest in the use of biological control agents to control plant-parasitic nematodes. The development of nematophagous fungi as biological control agents has revealed a need for further understanding of their infection processes. The egg-parasitic fungi, Pochonia chlamydosporia and Paecilomyces lilacinus, and the nematode trapping fungus, Arthrobotrys oligospora, have received the most attention. Through the application of biochemistry and molecular biology, aspects of their infection processes have been elucidated. This has involved the characterisation of enzymes that aid penetration of the eggshell or the nematode body wall and the identification of nematicidal toxins. This growing understanding of the biology of infection is opening new avenues in the improvement of fungi as biological control agents.

scholarly journals Mycofumigation by the Volatile Organic Compound-Producing Fungus Muscodor albus Induces Bacterial Cell Death through DNA Damage

2014 ◽  
Vol 81 (3) ◽  
pp. 1147-1156 ◽  
Author(s):  
Cambria J. Alpha ◽  
Manuel Campos ◽  
Christine Jacobs-Wagner ◽  
Scott A. Strobel
Keyword(s):  
Dna Damage ◽  
Mode Of Action ◽  
Plant Pathogens ◽  
Gene Knockout ◽  
Single Gene ◽  
Complex Mixture ◽  
Commercial Application ◽  
Content Type ◽  
Volatile Organic ◽  
Muscodor Albus

ABSTRACTMuscodor albusbelongs to a genus of endophytic fungi that inhibit and kill other fungi, bacteria, and insects through production of a complex mixture of volatile organic compounds (VOCs). This process of mycofumigation has found commercial application for control of human and plant pathogens, but the mechanism of the VOC toxicity is unknown. Here, the mode of action of these volatiles was investigated through a series of genetic screens and biochemical assays. A single-gene knockout screen revealed high sensitivity forEscherichia colilacking enzymes in the pathways of DNA repair, DNA metabolic process, and response to stress when exposed to the VOCs ofM. albus. Furthermore, the sensitivity of knockouts involved in the repair of specific DNA alkyl adducts suggests that the VOCs may induce alkylation. Evidence of DNA damage suggests that these adducts lead to breaks during DNA replication or transcription if not properly repaired. Additional cytotoxicity profiling indicated that during VOC exposure,E. colibecame filamentous and demonstrated an increase in cellular membrane fluidity. The volatile nature of the toxic compounds produced byM. albusand their broad range of inhibition make this fungus an attractive biological agent. Understanding the antimicrobial effects and the VOC mode of action will inform the utility and safety of potential mycofumigation applications forM. albus.

scholarly journals Impact of the omic technologies for understanding the modes of action of biological control agents against plant pathogens

BioControl ◽  
2015 ◽  
Vol 60 (6) ◽  
pp. 725-746 ◽  
Author(s):  
Sebastien Massart ◽  
Michele Perazzolli ◽  
Monica Höfte ◽  
Ilaria Pertot ◽  
M. Haïssam Jijakli
Keyword(s):  
Biological Control ◽  
Plant Pathogens ◽  
Biological Control Agents ◽  
Modes Of Action

scholarly journals Exploration and Identification Trichoderma spp. as a Biological Control Agents to Plant Pathogens and Starter Making Biological Fertilizers

2020 ◽  
Vol 13 (1) ◽  
pp. 222-226
Author(s):  
Henny V.G. Makal ◽  
Max M. Ratulangi ◽  
Denny S. Sualang
Keyword(s):  
Biological Control ◽  
Population Density ◽  
Plant Pathogens ◽  
Soil Samples ◽  
Dilution Method ◽  
Biological Control Agents ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Trichoderma Sp ◽  
Biological Fertilizer

The objectives of this study are: (1) to inventory Trichoderma spp. in North Minahasa District, South Minahasa District, and Tomohon City-Minahasa District, (2) inventory of Trichoderma spp. in the rhizosphere of cultivated and fallow gardens, and (3) calculate the population density of Trichoderma spp. all soil samples. The scope of this study is the biological control of plant pathogens, induce plant resistance, and biological fertilizer production. Trichoderma isolation spp. has been carried out by dilution method and cultured on PDA + antibiotics. Population density of Trichoderma spp. calculated using the plate calculation method. Identification of this species function based on the color and patterns of sporulation in the colony; hyphae and clamydospores; conidiophores; and phialides and phialospores. Trichoderma species found in North Minahasa District were T. harzianum, T. koningii, and T. viride; in South Minahasa District, T. koningii and T. viride; and in Tomohon City-Minahasa District, T. koningii and T. viride. In fallow gardens were T. harzianum, T. koningii, and T. viride, and in cultivated gardens were T. koningii and T. viride. Population densities of Trichoderma sp. in South Minahasa District, North Minahasa District, and Tomohon City-Minahasa District, respectively 1,363.64, 466.67, and 26.67 CFU / g soil.

scholarly journals Draft Genome Sequence of Chromobacterium subtsugae MWU12-2387 Isolated from a Wild Cranberry Bog in Truro, Massachusetts

2017 ◽  
Vol 5 (12) ◽  
Author(s):  
Kristin Vöing ◽  
Alisha Harrison ◽  
Scott D. Soby
Keyword(s):  
Biological Control ◽  
Genome Sequence ◽  
Virulence Genes ◽  
Insect Pests ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Biological Control Agents ◽  
Content Type ◽  
Bog Soils

ABSTRACT Chromobacterium subtsugae MWU12-2387 was isolated from the rhizosphere of cranberry plants. While it is unknown what environmental role these bacteria play in bog soils, they hold potential as biological control agents against nematodes and insect pests. Potential virulence genes were identified, including the violacein synthesis pathway, siderophores, and several chitinases.

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