Controlling Synthetic Cell-Cell Communication

Synthetic cells, which mimic cellular function within a minimal compartment, are finding wide application, for instance in studying cellular communication and as delivery devices to living cells. However, to fully realise the potential of synthetic cells, control of their function is vital. An array of tools has already been developed to control the communication of synthetic cells to neighbouring synthetic cells or living cells. These tools use either chemical inputs, such as small molecules, or physical inputs, such as light. Here, we examine these current methods of controlling synthetic cell communication and consider alternative mechanisms for future use.

Harnessing soil bacteria and their benefits for sustainable agriculture with changing climate.

Soil bacteria contribute effectively to key biogeochemical reactions in the soil rhizosphere. They support plants in the rhizosphere to adapt quickly to changing climatic conditions. Differences in root exudates, trace gas chemistry, chemical compounds and nutrient exchange contribute to the recruitment of diverse microorganisms by plant roots. This review highlights the importance of characterizing novel microorganisms to support sustainable agricultural practices. We discuss about tools for characterizing microbes and agricultural practices that influence microbial diversity, and have reviewed how microorganisms may have important but unidentified roles in climate change. Beneficial microbes could improve the turnover of carbon, nitrogen, phosphorus and other minerals thereby avoiding the use of chemical inputs, which are not only causing serious environmental harm but also pose danger to human and animal health.

Yeast and Filamentous Fungi Microbial Communities in Organic Red Grape Juice: Effect of Vintage, Maturity Stage, SO2, and Bioprotection

Changes are currently being made to winemaking processes to reduce chemical inputs [particularly sulfur dioxide (SO2)] and adapt to consumer demand. In this study, yeast growth and fungal diversity were investigated in merlot during the prefermentary stages of a winemaking process without addition of SO2. Different factors were considered, in a two-year study: vintage, maturity level and bioprotection by the adding yeast as an alternative to SO2. The population of the target species was monitored by quantitative-PCR, and yeast and filamentous fungi diversity was determined by 18S rDNA metabarcoding. A gradual decrease of the α-diversity during the maceration process was highlighted. Maturity level played a significant role in yeast and fungal abundance, which was lower at advanced maturity, while vintage had a strong impact on Hanseniaspora spp. population level and abundance. The presence of SO2 altered the abundance of yeast and filamentous fungi, but not their nature. The absence of sulfiting led to an unexpected reduction in diversity compared to the presence of SO2, which might result from the occupation of the niche by certain dominant species, namely Hanseniaspora spp. Inoculation of the grape juice with non-Saccharomyces yeast resulted in a decrease in the abundance of filamentous fungi generally associated with a decline in grape must quality. Lower abundance and niche occupation by bioprotection agents were observed at the overripened stage, thus suggesting that doses applied should be reconsidered at advanced maturity. Our study confirmed the bioprotective role of Metschnikowia pulcherrima and Torulaspora delbrueckii in a context of vinification without sulfites.

Innovativity in Legislative, Political and Organizational Frameworks of Sustainable Land Management in Benin

Agricultural lands are increasingly degraded due to various human actions (overgrazing, intensive use of chemical inputs) and the consequences of climate change. In Benin, 62% of agricultural land were degraded in 2017. In this regard, Sustainable Land Management measures are highlighted by various public decisions. This study analyzed the political, legislative, and organizational frameworks of Sustainable Land Management in Benin. It was carried out through content analysis of laws, decrees, and Sustainable Land Management policy documents to highlight the place of innovativity in institutional and organizational framework in Sustainable Land Management in Benin. Results reveal that the various frameworks analyzed are oriented towards developing practice-based innovations through the implementation of Sustainable Land Management measures, support to actors in the implementation of Sustainable Land Management innovations and the monitoring and evaluation of Sustainable Land Management strategies. The capacity to innovate is also promoted through the strengthening of the technical, institutional, material, and financial capacities of the actors. On the other hand, the propensity to innovate component of Innovativity is hardly addressed in Sustainable Land Management public policies in Benin. It is therefore necessary to direct agricultural policies towards an institutionalization of the strengthening of the propensity to innovate of agricultural producers for a long-term appropriation of Sustainable Land Management measures in Benin.

Evaluation of the Sustainability of Urban Market Gardens in the City of Meknes (Morocco)

Market gardening constitutes a basis for food security of urban and peri-urban households. However, market gardening is subject to the use of chemical inputs, selected varieties, and genetically modified organisms. These practices are increasingly controversial and are likely to affect the sustainability of vegetable farms. This study assessed the sustainability of 120 randomly selected market garden farms (Meknes) using the surveys and the market garden production sustainability indicator. The results show that the majority of the farms identified are characterized by low sustainability, with the agroecological dimension being the limiting factor. The improvement of the components "Ecological diversity", "Spatial organization", and "Agricultural practices" are the ways to improve the global sustainability of vegetable production in Meknes. The socio-territorial sustainability of production is characterized by a lack of training for producers, a lack of hygiene and safety in production activities, and a low contribution to employment. The economic dimension is characterized by low viability, low economic transmissibility, and financial autonomy. To perpetuate the market gardening in the city of Meknes, it appears necessary to promote ecological intensification, integrated diversification, and promotion of local inputs to the various types of market gardening farms

Unmanned Aerial System-Based Weed Mapping in Sod Production Using a Convolutional Neural Network

Weeds are a persistent problem on sod farms, and herbicides to control different weed species are one of the largest chemical inputs. Recent advances in unmanned aerial systems (UAS) and artificial intelligence provide opportunities for weed mapping on sod farms. This study investigates the weed type composition and area through both ground and UAS-based weed surveys and trains a convolutional neural network (CNN) for identifying and mapping weeds in sod fields using UAS-based imagery and a high-level application programming interface (API) implementation (Fastai) of the PyTorch deep learning library. The performance of the CNN was overall similar to, and in some classes (broadleaf and spurge) better than, human eyes indicated by the metric recall. In general, the CNN detected broadleaf, grass weeds, spurge, sedge, and no weeds at a precision between 0.68 and 0.87, 0.57 and 0.82, 0.68 and 0.83, 0.66 and 0.90, and 0.80 and 0.88, respectively, when using UAS images at 0.57 cm–1.28 cm pixel–1 resolution. Recall ranges for the five classes were 0.78–0.93, 0.65–0.87, 0.82–0.93, 0.52–0.79, and 0.94–0.99. Additionally, this study demonstrates that a CNN can achieve precision and recall above 0.9 at detecting different types of weeds during turf establishment when the weeds are mature. The CNN is limited by the image resolution, and more than one model may be needed in practice to improve the overall performance of weed mapping.

Reduction of Colour from effluents of Pulp and Paper Industry by Ozonation: A Review

Pulp and paper making is the major old process industry in India which is water intensive and generates heavy water pollution. Pulp and paper industries are the fifth largest contributor to industrial water pollution. Waste water is generated from each and every section of paper making process and depends upon the type of pulping and bleaching process. Presently, primary and secondary (Biological) treatment systems based on activated sludge process are widely used by paper industry for effluent treatment. The process requires high energy and chemical inputs and involves high operational costs. One of the novel processes for treating effluent is its oxidation through ozonation which is a greener way of degrading pollutants. Ozonation of intermediate stage effluents having high colour load is more effective for industrial application and re-utilization.

Studies on insect diversity in jasmine (Jasminum sambac L.) ecosystem

Aim: Study was carried out to record the abundance and diversity of insects in jasmine (Jasminum sambac L.) ecosystem. Methodology: To determine insect pests, natural enemies and non-insect diversity, fortnight observations were taken from second fortnight of September'19 to March'20 on insect population at three adjacent locations. Abundance and diversity indices were computed using mean data. The management practices, presence of weeds and adjacent cropping systems were also recorded at three locations to determine the reasons for variation in the diversity of organisms. Results: A total of 3335 individuals arthropods belonging to 55 species, which included 32 species of herbivores and 23 species of natural enemies were recorded throughout the study period. Location 3 had perennial crops in the vicinity, un-weeded ecosystem with no chemical inputs. Among various diversity indices calculated, Margalef Richness Index of herbivores was higher at location 3 (1.67) than location 1 (1.17) and 2 (0.76). Interpretation: The abundance and diversity of insects in different jasmine ecosystem depends on the chemical inputs and adjacent cropping system.

2-Nitro- and 4-fluorocinnamaldehyde based receptors as naked-eye chemosensors to potential molecular keypad lock

New-generation chemosensors desire small organic molecules that are easy to synthesise and cost-effective. As a new interdisciplinary area of research, the integration of these chemosensors into keypad locks or other advanced communication protocols is becoming increasingly popular. Our lab has developed new chemosensor probes that contain 2-nitro- (1–3) and 4-fluoro-cinnamaldehyde (4–6) and applied them to the anion recognition and sensing process. Probes 1–6 are colorimetric sensors for naked-eye detection of AcO−/CN−/F−, while probes 4–6 could differentiate between F− and AcO−/CN− anions in acetonitrile. Using the density functional theory (DFT), it was found that probes 1–6 acted as effective chemosensors. By using Probe 5 as a chemosensor, we explored colorimetric recognition of multiple anions in more detail. Probe 5 was tested in combination with a combinatorial approach to demonstrate pattern-generation capability and its ability to distinguish among chemical inputs based on concentration. After pattern discrimination using principal component analysis (PCA), we examined anion selectivity using DFT computation. In our study, probe 5 demonstrates excellent performance as a chemosensor and shows promise as a future molecular-level keypad lock system.