Barcoded Primers Used in Multiplex Amplicon Pyrosequencing Bias Amplification
2011 ◽
Vol 77
(21)
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pp. 7846-7849
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Keyword(s):
ABSTRACT“Barcode-tagged” PCR primers used for multiplex amplicon sequencing generate a thus-far-overlooked amplification bias that produces variable terminal restriction fragment length polymorphism (T-RFLP) and pyrosequencing data from the same environmental DNA template. We propose a simple two-step PCR approach that increases reproducibility and consistently recovers higher genetic diversity in pyrosequencing libraries.
1996 ◽
Vol 42
(11)
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pp. 1121-1130
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2005 ◽
Vol 19
(4)
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pp. 267-274
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2006 ◽
Vol 53
(1)
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pp. 187-195
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2016 ◽
Vol 5
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pp. S174-S175
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1996 ◽
Vol 62
(3)
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pp. 979-985
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