scholarly journals Hemagglutinating Activity of Enteroviruses Recovered in Two Primary Cell Lines and a Stable Cell Line

1968 ◽  
Vol 16 (5) ◽  
pp. 703-707 ◽  
Author(s):  
K. R. Berquist ◽  
Suzanne P. Fritts
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Hemagglutinating Activity ◽  
Primary Cell ◽  
Stable Cell Line ◽  
Primary Cell Lines

The Cellular Substrate: A Very Important Requirement for Baculovirus in vitro Replication

1984 ◽  
Vol 39 (9-10) ◽  
pp. 993-1002 ◽  
Author(s):  
Herbert G. Miltenburger ◽  
Werner L. Naser ◽  
Jeanne P. Harvey ◽  
Jürg Huber ◽  
Alois M. Huger
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cydia Pomonella ◽  
Codling Moth ◽  
Primary Cell ◽  
Insect Species ◽  
In Vitro Replication ◽  
Primary Cell Lines ◽  
First Time

Abstract We established more than 200 primary cell lines of Cydia pomonella (codling moth). 81 of them were selected and screened for replication of two baculoviruses (from two different subgroups): the Choristoneura murinana NPV and the Cydia pomonella GV. Although all these cell lines had been derived from the same insect species, they varied largely in their response to challenge with the NPV. Most of them showed CPE or produced different amounts of poly-hedra. Interestingly, we also found a few cell lines that were permissive for GV replication. To our knowledge this is the first time that GV replication in cell lines has been obtained. Our results show that cell line properties are most important for baculovirus in vitro replication.

scholarly journals In vitro and in vivo effect of human lactoferrin on glioblastoma growth

2015 ◽  
Vol 123 (4) ◽  
pp. 1026-1035 ◽  
Author(s):  
Antonietta Arcella ◽  
Maria Antonietta Oliva ◽  
Sabrina Staffieri ◽  
Silvia Aalberti ◽  
Giovanni Grillea ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Nude Mice ◽  
Tumor Size ◽  
Primary Cell ◽  
Continuous Cell Line ◽  
Human Lactoferrin ◽  
Primary Cell Lines

OBJECT Human lactoferrin (HLF) is a natural protein with antitumor activity. The aim of this study was to investigate the effects of HLF alone and in combination with temozolomide (TMZ), a conventional chemotherapeutic, on human glioblastoma (GBM) cells. METHODS The authors cultured fresh human primary cell lines NMD and FN and the continuous cell line U87MG to evaluate proliferation in the presence of HLF alone at different doses (1, 10, and 100 mg/ml, and 1 mg/ml) and in combination with TMZ. In in vivo experiments they assessed tumor size reduction in CD1 nude mice carrying an orthotopic GBM xenograft and orally treated with HLF. RESULTS Lactoferrin causes growth inhibition in the NMD and FN primary cell lines and in the U87MG continuous cell line. This inhibition seemed to be modulated by the downregulation of cyclin D1 and D4. Western blot and fluorescence-activated cell sorting analysis showed inhibition of the cell cycle in G0/G1 and G2 phases. When administered in nude mice, HLF (60 mg/kg/day) decreased tumor size about 30%, as shown in both histological analyses and high-field brain MRI. Administration of HLF with TMZ enhanced the effect of chemotherapy both in vitro and in vivo. CONCLUSIONS This study demonstrated that HLF can inhibit GBM cell growth, suggesting that this nontoxic substance may have a role in potentiating the effect of current TMZ treatment of GBM.

Ectodermal and ectomesenchymal marker expression in primary cell lines of complex and compound odontomas: a pilot study

2019 ◽  
Vol 68 (3) ◽  
Author(s):  
David A. Trejo-Remigio ◽  
Luis F. Jacinto-Alemán ◽  
Elba R. Leyva-Huerta ◽  
Bogdan R. Navarro-Bustos ◽  
Javier Portilla-Robertson
Keyword(s):  
Pilot Study ◽  
Cell Lines ◽  
Primary Cell ◽  
Primary Cell Lines ◽  
Marker Expression

scholarly journals Analysis of a transgenic mouse containing simian virus 40 and v-myc sequences.

1985 ◽  
Vol 5 (4) ◽  
pp. 642-648 ◽  
Author(s):  
J A Small ◽  
D G Blair ◽  
S D Showalter ◽  
G A Scangos
Keyword(s):  
Cell Lines ◽  
Choroid Plexus Papilloma ◽  
Simian Virus 40 ◽  
Soft Agar ◽  
Simian Virus ◽  
T Antigen ◽  
Primary Cell ◽  
Tissue Samples ◽  
Primary Cell Lines

Two plasmids, one containing the simian virus 40 (SV40) genome and the mouse metallothionein I gene and one containing the v-myc gene of avian myelocytomatosis virus MC29, were coinjected into mouse embryos. Of the 13 surviving mice, one, designated M13, contained both myc and SV40 sequences. This mouse developed a cranial bulge identified as a choroid plexus papilloma at 13 weeks and was subsequently sacrificed; tissue samples were taken for further analysis. Primary cell lines derived from these tissues contained both myc and SV40 DNA. No v-myc mRNA could be detected, although SV40 mRNA was present in all of the cell lines tested. T antigen also was expressed in all of the cell lines analyzed. These data suggest that SV40 expression was involved in the abnormalities of mouse M13 and was responsible for the transformed phenotype of the primary cell lines. Primary cell lines from this mouse were atypical in that the population rapidly became progressively more transformed with time in culture based on the following criteria: morphology, growth rate, and the ability to grow in soft agar and in serum-free medium. The data also suggest that factors present in the mouse regulated the ability of SV40 to oncogenically transform most cells and that in vitro culture of cells allowed them to escape those factors.

In vitro effects of erythromycin and florfenicol on primary cell lines of Unio crassus and Cyprinus carpio

2021 ◽  
Author(s):  
Pınar Arslan ◽  
Begum Yurdakok-Dikmen ◽  
Saniye Cevher Ozeren ◽  
Ozgur Kuzukiran ◽  
Ayhan Filazi
Keyword(s):  
Cell Lines ◽  
Cyprinus Carpio ◽  
Primary Cell ◽  
Primary Cell Lines ◽  
In Vitro Effects

scholarly journals PO-481 Patient-derived sarcoma primary cell lines with preserved cancer stem cell properties to screen anticancer compounds

2018 ◽  
Author(s):  
V Rey Vázquez ◽  
L Fernández Nevado ◽  
S Tirados Menéndez ◽  
Ó Estupiñán Sánchez ◽  
R Rodríguez González
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Cell Lines ◽  
Primary Cell ◽  
Anticancer Compounds ◽  
Primary Cell Lines

scholarly journals TMOD-01. CHARACTERIZATION OF PATIENT-DERIVED PRIMARY CELL LINES AND XENOGRAFTS FOR GLIOBLASTOMA

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi262-vi262 ◽  
Author(s):  
Noriyuki Kijima ◽  
Daisuke Kanematsu ◽  
Tomoko Shofuda ◽  
Masahiro Nonaka ◽  
Ryoichi Iwata ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Surface ◽  
Cell Lines ◽  
Primary Cell Culture ◽  
Surface Marker ◽  
Primary Cell ◽  
Cell Surface Marker ◽  
High Expression ◽  
Glioblastoma Patient ◽  
Primary Cell Lines

Abstract Patient-derived primary cell culture and xenograft are essential tools for translational research for glioblastoma. However, characteristics of each patient derived cell line and xenograft is not extensively studied. In this study, we aim to analyze the characteristics of our glioblastoma patient-derived cell lines and xenografts based on cell surface markers and their differentiation patterns. We have established 20 glioblastoma primary cell culture lines by serum free medium containing EGF and bFGF and found that primary cell culture lines could be classified based on the expression of CD133 and CD44. Four cell lines had high expression of both CD133 and CD44. Eleven cell lines had high expression of only CD44, three cell lines had high expression of only CD133, two cell lines had low expression of both CD133 and CD44. In addition when we induce differentiation, these cell lines showed differentiation to both glial and neuronal differentiation, but differentiation patterns were different depending on each cell line. Four cell lines showed predominant neuronal differentiation and others showed predominant glial differentiation. We next investigated in vivo characteristics of glioblastoma patient derived xenografts from these established cell lines. We have injected these cell lines into NOD/Shi-scid IL2Rγ KO mouse and histopathologically analyzed characteristics of xenografts. Each xenograft well recapitulated histological features of original patients’ tumors and tumor cells remarkably invade through subventricular zone. These results suggest that glioblastoma patient derived primary cell lines and xenografts have different characteristics of cell surface marker expressions and differentiation patterns, thus can classify these cell lines depending on cell surface marker expressions and differentiation patterns. Further analysis is needed to examine the biological importance of the differences in cell surface marker expressions and differentiation patterns.

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