Optimization of the Sistrom Culture Medium for Large-Scale Batch Cultivation of Rhodospirillum rubrum under Semiaerobic Conditions with Maximal Yield of Photosynthetic Membranes

Bamboo species are an alternative for the composition of forest plantations. However, their potential has not been explored due to the hard time in producing large-scale clonal plants. Thus, the aim this work was to evaluate the in vitro establishment, bud multiplication and ex vitro rooting of Bambusa vulgaris. The first experiment tested different systemic and contact fungicide solutions, based on exposure time, during the establishment phase. Established explants were subjected to evaluation of residual fungicide effect on subcultures during the multiplication and elongation phases. The second experiment evaluated the influence of activated carbon on ex vitro survival and on adventitious rooting. Explant immersion in liquid culture medium added with 1.0 mL of fungicide for 120 hours has favored the in vitro establishment and reduced fungal contamination. On the other hand, it favored the shoot emission of shoots per explant during the multiplication phase. Both rooting induction culture medium and mini-incubator system use were effective in enabling adventitious root formation. The presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.

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Expansion of Human Pluripotent Stem Cell-derived Early Cardiovascular Progenitor Cells by a Cocktail of Signaling Factors

Scientific Reports ◽

10.1038/s41598-019-52516-8 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 3

Author(s):

Sadaf Vahdat ◽

Sara Pahlavan ◽

Elena Mahmoudi ◽

Maryam Barekat ◽

Hassan Ansari ◽

...

Keyword(s):

Progenitor Cells ◽

Large Scale ◽

Culture Medium ◽

Gene Expression Pattern ◽

Extended Period ◽

Scale Production ◽

Chemical Screening ◽

Wide Range ◽

Cell Sources

Abstract Cardiovascular progenitor cells (CPCs) derived from human pluripotent stem cells (hPSCs) are proposed to be invaluable cell sources for experimental and clinical studies. This wide range of applications necessitates large-scale production of CPCs in an in vitro culture system, which enables both expansion and maintenance of these cells. In this study, we aimed to develop a defined and efficient culture medium that uses signaling factors for large-scale expansion of early CPCs, called cardiogenic mesodermal cells (CMCs), which were derived from hPSCs. Chemical screening resulted in a medium that contained a reproducible combination of three factors (A83-01, bFGF, and CHIR99021) that generated 1014 CMCs after 10 passages without the propensity for tumorigenicity. Expanded CMCs retained their gene expression pattern, chromosomal stability, and differentiation tendency through several passages and showed both the safety and possible cardio-protective potentials when transplanted into the infarcted rat myocardium. These CMCs were efficiently cryopreserved for an extended period of time. This culture medium could be used for both adherent and suspension culture conditions, for which the latter is required for large-scale CMC production. Taken together, hPSC-derived CMCs exhibited self-renewal capacity in our simple, reproducible, and defined medium. These cells might ultimately be potential, promising cell sources for cardiovascular studies.

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Reduction of Selenite and Detoxification of Elemental Selenium by the Phototrophic BacteriumRhodospirillum rubrum

Applied and Environmental Microbiology ◽

10.1128/aem.65.11.4734-4740.1999 ◽

1999 ◽

Vol 65 (11) ◽

pp. 4734-4740 ◽

Cited By ~ 150

Author(s):

J. Kessi ◽

M. Ramuz ◽

E. Wehrli ◽

M. Spycher ◽

R. Bachofen

Keyword(s):

Cell Wall ◽

Plasma Membrane ◽

Cell Density ◽

Culture Medium ◽

Rhodospirillum Rubrum ◽

Buoyant Density ◽

Elemental Selenium ◽

Selenite Reduction ◽

Final Cell Density ◽

Cell Densities

ABSTRACT The effect of selenite on growth kinetics, the ability of cultures to reduce selenite, and the mechanism of detoxification of selenium were investigated by using Rhodospirillum rubrum. Anoxic photosynthetic cultures were able to completely reduce as much as 1.5 mM selenite, whereas in aerobic cultures a 0.5 mM selenite concentration was only reduced to about 0.375 mM. The presence of selenite in the culture medium strongly affected cell division. In the presence of a selenite concentration of 1.5 mM cultures reached final cell densities that were only about 15% of the control final cell density. The cell density remained nearly constant during the stationary phase for all of the selenite concentrations tested, showing that the cells were not severely damaged by the presence of selenite or elemental selenium. Particles containing elemental selenium were observed in the cytoplasm, which led to an increase in the buoyant density of the cells. Interestingly, the change in the buoyant density was reversed after selenite reduction was complete; the buoyant density of the cells returned to the buoyant density of the control cells. This demonstrated that R. rubrum expels elemental selenium across the plasma membrane and the cell wall. Accordingly, electron-dense particles were more numerous in the cells during the reduction phase than after the reduction phase.

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Demonstration of two 3,3'-diaminobenzidine oxidation reactions associated with photosynthetic membranes in anaerobic light-grown Rhodospirillum rubrum.

Journal of Histochemistry & Cytochemistry ◽

10.1177/25.11.410873 ◽

1977 ◽

Vol 25 (11) ◽

pp. 1264-1268 ◽

Cited By ~ 2

Author(s):

F J Stevens ◽

H S Pankratz ◽

R L Uffen

Keyword(s):

Molecular Oxygen ◽

White Light ◽

Rhodospirillum Rubrum ◽

Oxidation Reactions ◽

Photosynthetic Membranes ◽

Dependent Activity ◽

Two Systems

Photosynthetic membranes of anaerobic light-grown Rhodospirillum rubrum oxidized 3,3'-diaminobenzidine. When glutaraldehyde-treated cells were exposed to 3,3'-diaminobenzidine in the light aerobically, the oxidation appeared to occur by two systems. One reaction was stimulated by white light and the second required molecular oxygen. The O2-dependent activity was inhibited by KCN.

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Short-term storage in vitro and large-scale propagation of grapevine genotypes

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2012000300005 ◽

2012 ◽

Vol 47 (3) ◽

pp. 344-350 ◽

Cited By ~ 9

Author(s):

Rafael de Carvalho Silva ◽

Zanderluce Gomes Luis ◽

Jonny Everson Scherwinski-Pereira

Keyword(s):

Survival Rate ◽

Large Scale ◽

Culture Medium ◽

Short Term ◽

Minimal Growth ◽

Short Term Storage ◽

Term Storage ◽

Storage Temperatures ◽

Number Of Shoots

The objective of this work was to evaluate the large-scale propagation of grapevine genotypes after short-term storage in vitro. Microshoots from ten grapevine genotypes were used. The following storage temperatures were evaluated: 10, 20, and 25°C. After short-term storage, the shoots were propagated in up to five successive subcultures, to assess the large-scale propagation of the germplasm maintained under conditions of minimal growth. The propagated shoots were rooted in different concentrations of indolbutiric acid (IBA) and acclimatized in greenhouse. The best temperature for short-term storage in vitro and survival of the genotypes was 20°C. In the propagation phase, the highest number of shoots per explant was found in the subcultures 4 and 5, with averages of 4.9 and 4.8 shoots per explant, respectively. In the rooting phase, the best results for number of roots were obtained using a culture medium supplemented with 0.4 µmol L-1 of IBA, with an average of three roots per shoot. During the acclimation phase, a survival rate higher than 95% was achieved after 30 days in the greenhouse. Grapevine genotypes maintained for six months in vitro, at 20ºC, can be micropropagated in large scale.

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Spirulina Culture in Bangladesh XI: Selection of a Culture Medium, Suitable for Culturing a Local Strain of Spirulina

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v41i3.293 ◽

1970 ◽

Vol 41 (3) ◽

pp. 227-234

Author(s):

R Khatun ◽

P Noor ◽

N Akhter ◽

MAA Jahan ◽

M Hossain ◽

...

Keyword(s):

Spirulina Platensis ◽

Climatic Condition ◽

Large Scale ◽

Culture Medium ◽

Culture Media ◽

Local Strain ◽

Condition Effect ◽

Selection Of

Large scale Spirulina culture is possible in Bangladesh inspite of unpredictable climatic condition. Effect of different culture media on the growth of a local strain of Spirulina (Spirulina platensis) was studied. Bangladesh medium (Bd1) was found to be more favourable for the growth of the alga. All together three culture media were included in this study. Bangladesh J. Sci. Ind. Res. 41(3-4), 227-234, 2006

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Biomass of a Psychrophilic Fungus as a Biocatalyst for Efficient Direct Esterification of Citronellol

BioEnergy Research ◽

10.1007/s12155-021-10289-x ◽

2021 ◽

Author(s):

Mateusz Kutyła ◽

Mariusz Trytek ◽

Katarzyna Buczek ◽

Ewa Tomaszewska ◽

Siemowit Muszyński

Keyword(s):

Large Scale ◽

Culture Medium ◽

Low Cost ◽

Molar Ratio ◽

High Catalytic Activity ◽

Active Biomass ◽

Direct Esterification ◽

Citronellyl Acetate ◽

Biomass Activity ◽

Psychrophilic Fungus

AbstractA biomass-bound lipase from psychrophilic Chrysosporium pannorum A-1 is an efficient biocatalyst for direct esterification of β-citronellol and acetic acid in an organic solvent. The biomass is effectively produced by fungal submerged culture at 20 ℃, which results in lower energy consumption during the production of biocatalyst. Supplementation of the culture medium with calcium carbonate together with olive oil contributed to a significant increase in the active biomass of mycelium in one batch culture and increased the efficiency of the biocatalyst. Biomass-bound lipase showed high catalytic activity in a broad temperature range of 30–60 °C and stability up to 70 °C. A maximum molar conversion value of 98% was obtained at 30 °C in n-hexane using a 2:1 alcohol-to-acid molar ratio and 3% w/v of the biocatalyst within 24 h. The high equimolar concentration of the substrates (200 mM) did not have an adverse effect on mycelial biomass activity. Dry mycelium of C. pannorum is a promising biocatalyst for large-scale biosynthesis of citronellyl acetate, given its low-cost production, high activity at low temperatures, and reusability in a minimum of seven 24-h biocatalytic cycles.

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