scholarly journals Molecular Cloning, Characterization, and Differential Expression of a Glucoamylase Gene from the Basidiomycetous Fungus Lentinula edodes

2000 ◽  
Vol 66 (6) ◽  
pp. 2531-2535 ◽  
Author(s):  
J. Zhao ◽  
Y. H. Chen ◽  
H. S. Kwan
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Catalytic Domain ◽  
Coding Region ◽  
Fruiting Body Formation ◽  
Mycelium Growth ◽  
Body Formation ◽  
Basidiomycetous Fungus ◽  
Glucoamylase Gene

ABSTRACT The complete nucleotide sequence of putative glucoamylase genegla1 from the basidiomycetous fungus Lentinula edodes strain L54 is reported. The coding region of the genomic glucoamylase sequence, which is preceded by eukaryotic promoter elements CAAT and TATA, spans 2,076 bp. The gla1 gene sequence codes for a putative polypeptide of 571 amino acids and is interrupted by seven introns. The open reading frame sequence of thegla1 gene shows strong homology with those of other fungal glucoamylase genes and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The similarity between the Gla1 protein and other fungal glucoamylases is from 45 to 61%, with the region of highest conservation found in catalytic domains and starch-binding domains. We compared the kinetics of glucoamylase activity and levels of gene expression in L. edodes strain L54 grown on different carbon sources (glucose, starch, cellulose, and potato extract) and in various developmental stages (mycelium growth, primordium appearance, and fruiting body formation). Quantitative reverse transcription PCR utilizing pairs of primers specific forgla1 gene expression shows that expression ofgla1 was induced by starch and increased during the process of fruiting body formation, which indicates that glucoamylases may play an important role in the morphogenesis of the basidiomycetous fungus.

scholarly journals Coupling gene expression and multicellular morphogenesis during fruiting body formation in Myxococcus xanthus

2003 ◽  
Vol 48 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Lotte Søgaard-Andersen ◽  
Martin Overgaard ◽  
Sune Lobedanz ◽  
Eva Ellehauge ◽  
Lars Jelsbak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Myxococcus Xanthus ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Multicellular Morphogenesis

scholarly journals Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1045
Author(s):  
Lianlian Yan ◽  
Ruiping Xu ◽  
Yinbing Bian ◽  
Hongxian Li ◽  
Yan Zhou
Keyword(s):  
Gene Family ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Expression Profiles ◽  
Draft Genome ◽  
White Rot ◽  
Laccase Gene ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Laccase Genes

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

scholarly journals SigF, a New Sigma Factor Required for a Motility System of Myxococcus xanthus

2005 ◽  
Vol 187 (24) ◽  
pp. 8537-8541 ◽  
Author(s):  
Toshiyuki Ueki ◽  
Chun-Ying Xu ◽  
Sumiko Inouye
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Sigma Factor ◽  
Vegetative Growth ◽  
Growth Analysis ◽  
Myxococcus Xanthus ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
The Social ◽  
Social Motility

ABSTRACT A new sigma factor, SigF, was identified from the social and developmental bacterium Myxococcus xanthus. SigF is required for fruiting body formation during development as well as social motility during vegetative growth. Analysis of gene expression indicates that it is possible that the sigF gene is involved in regulation of an unidentified gene for social motility.

scholarly journals Mapping of genes abundantly expressed during fruiting body formation of Lentinula edodes

2010 ◽  
Vol 60 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Kazuhiro Miyazaki ◽  
Miho Sakai ◽  
Yasumasa Miyazaki
Keyword(s):  
Fruiting Body ◽  
Lentinula Edodes ◽  
Fruiting Body Formation ◽  
Body Formation

HthA, a putative DNA-binding protein, and HthB are important for fruiting body morphogenesis in Myxococcus xanthus

Microbiology ◽  
2004 ◽  
Vol 150 (7) ◽  
pp. 2171-2183 ◽  
Author(s):  
Mette Nielsen ◽  
Anders Aa. Rasmussen ◽  
Eva Ellehauge ◽  
Anke Treuner-Lange ◽  
Lotte Søgaard-Andersen
Keyword(s):  
Gene Expression ◽  
Dna Binding ◽  
Fruiting Body ◽  
Binding Protein ◽  
Myxococcus Xanthus ◽  
Dna Binding Protein ◽  
Fruiting Body Formation ◽  
Developmental Defects ◽  
Body Formation ◽  
Developmental Gene Expression

In response to starvation, Myxococcus xanthus initiates a developmental programme that results in the formation of spore-filled multicellular fruiting bodies. Fruiting body formation depends on the temporal and spatial coordination of aggregation and sporulation and involves temporally and spatially coordinated changes in gene expression. This paper reports the identification of two genes, hthA and hthB, that are important for fruiting body formation. hthA and hthB are co-transcribed, and transcription of the two genes decreases strongly during development. Loss of HthA and HthB function results in delayed aggregation, a reduction in the level of sporulation, and abnormal developmental gene expression. Extracellular complementation experiments showed that the developmental defects caused by loss of HthA and HthB function are not due to the inability to synthesize an intercellular signal required for fruiting body formation. HthA, independent of HthB, is required for aggregation. HthB, alone or in combination with HthA, is required for sporulation. HthA is predicted to contain a C-terminal helix–turn–helix DNA-binding domain. Intriguingly, the N-terminal part of HthA does not exhibit significant amino acid similarity to proteins in the databases. The HthB protein lacks homologues in the databases. The results suggest that HthA is a novel DNA-binding protein, which regulates transcription of genes important for aggregation, and that HthB, alone or in combination with HthA, stimulates sporulation.

scholarly journals Modified recipe to inhibit GSK-3 for the living fungal biomaterial manufacture

2018 ◽  
Author(s):  
Chang Jinhui ◽  
Kathy PoLam Chan ◽  
Yichun Xie ◽  
Ka Lee Ma ◽  
Hoi Shan Kwan
Keyword(s):  
Fruiting Body ◽  
Environmental Changes ◽  
Low Cost ◽  
Fungal Mycelium ◽  
Self Healing ◽  
Fruiting Bodies ◽  
Fruiting Body Formation ◽  
Mycelium Growth ◽  
Innovative Strategy ◽  
Body Formation

Living fungal mycelium with suppressed or abolished fruit-forming ability is a self-healing substance particularly valuable biomaterial for further engineering and development in applications such as monitoring/sensing environmental changes and secreting signals. The ability to suppress fungal fruiting is also a useful tool for maintaining stability (e.g., shape, form) of a mycelium-based biomaterial with ease and lower cost. The objective of this present study is to provide a biochemical solution to regulate the fruiting body formation to replace heat killing of mycelium during production. We discovered that GSK-3 activity directly correlates with the development of fruiting bodies in fungi, especially mushroom forming fungi such as Coprinopsis cinerea. By regulating GSK-3 expression and activity, one can control the fungal fruiting body development. We successfully demonstrated that treatment of an inhibitor of GSK-3 kinase activity resulted in acceleration in mycelium growth rate, absence of fruiting body and general decrease in GSK-3 gene expression. Therefore, GSK-3 inhibitor is suggested to be included in the mycelium cultivation recipes for regulating the growth of fungal mycelium and for inhibiting the development of fruiting bodies. This is the first report of using a GSK-3 inhibitor, such as lithium or any other GSK-3 inhibitor, to suppress or abolish fruiting body formation in living fungal mycelium-based biomaterial. It also provides an innovative strategy for easy, reliable, and low cost maintenance of biomaterial containing live fungal mycelium.

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