scholarly journals Impact of UV Radiation on Bacterioplankton Community Composition

2001 ◽  
Vol 67 (2) ◽  
pp. 665-672 ◽  
Author(s):  
Christian Winter ◽  
Markus M. Moeseneder ◽  
Gerhard J. Herndl
Keyword(s):  
Uv Radiation ◽  
North Sea ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Potential Effect ◽  
Surface Mixed Layer ◽  
Marine Bacterioplankton ◽  
Bacterioplankton Community ◽  
The North ◽  
Coastal Marine ◽  
Bacterioplankton Communities

ABSTRACT The potential effect of UV radiation on the composition of coastal marine bacterioplankton communities was investigated. Dilution cultures with seawater collected from the surface mixed layer of the coastal North Sea were exposed to different ranges of natural or artificial solar radiation for up to two diurnal cycles. The composition of the bacterioplankton community prior to exposure was compared to that after exposure to the different radiation regimes using denaturing gradient gel electrophoresis (DGGE) of 16S rRNA and 16S ribosomal DNA. Only minor changes in the composition of the bacterial community in the different radiation regimes were detectable. Sequencing of selected bands obtained by DGGE revealed that some species of theFlexibacter-Cytophaga-Bacteroides (FCB) group were sensitive to UV radiation while other species of the FCB group were resistant. Overall, only up to ≈10% of the operational taxonomic units present in the dilution cultures appeared to be affected by UV radiation. Thus, we conclude that UV radiation has little effect on the composition of coastal marine bacterioplankton communities in the North Sea.

scholarly journals Diversity and activity of marine bacterioplankton during a diatom bloom in the North Sea assessed by total RNA and pyrotag sequencing

2014 ◽  
Vol 18 ◽  
pp. 185-192 ◽  
Author(s):  
Anna Klindworth ◽  
Alexander J. Mann ◽  
Sixing Huang ◽  
Antje Wichels ◽  
Christian Quast ◽  
...  
Keyword(s):  
North Sea ◽  
Diatom Bloom ◽  
Total Rna ◽  
Marine Bacterioplankton ◽  
The North ◽  
The North Sea

Rapid turnover of dissolved DMS and DMSP by defined bacterioplankton communities in the stratified euphotic zone of the North Sea

2002 ◽  
Vol 49 (15) ◽  
pp. 3017-3038 ◽  
Author(s):  
Mikhail V. Zubkov ◽  
Bernhard M. Fuchs ◽  
Stephen D. Archer ◽  
Ronald P. Kiene ◽  
Rudolf Amann ◽  
...  
Keyword(s):  
North Sea ◽  
Euphotic Zone ◽  
Rapid Turnover ◽  
The North ◽  
The North Sea ◽  
Bacterioplankton Communities

scholarly journals Metatranscriptome of marine bacterioplankton during winter time in the North Sea assessed by total RNA sequencing

2015 ◽  
Vol 19 ◽  
pp. 45-46 ◽  
Author(s):  
Anna Kopf ◽  
Ivaylo Kostadinov ◽  
Antje Wichels ◽  
Christian Quast ◽  
Frank Oliver Glöckner
Keyword(s):  
Rna Sequencing ◽  
North Sea ◽  
Total Rna ◽  
Marine Bacterioplankton ◽  
The North ◽  
The North Sea ◽  
Winter Time

scholarly journals Optimization of Terminal-Restriction Fragment Length Polymorphism Analysis for Complex Marine Bacterioplankton Communities and Comparison with Denaturing Gradient Gel Electrophoresis

1999 ◽  
Vol 65 (8) ◽  
pp. 3518-3525 ◽  
Author(s):  
Markus M. Moeseneder ◽  
Jesús M. Arrieta ◽  
Gerard Muyzer ◽  
Christian Winter ◽  
Gerhard J. Herndl
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
16S Rrna ◽  
Length Polymorphism ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Marine Bacterioplankton ◽  
Gradient Gel Electrophoresis ◽  
Restriction Fragment Length ◽  
Bacterioplankton Communities

ABSTRACT The potential of terminal-restriction fragment length polymorphism (T-RFLP) and the detection of operational taxonomic units (OTUs) by capillary electrophoresis (CE) to characterize marine bacterioplankton communities was compared with that of denaturing gradient gel electrophoresis (DGGE). A protocol has been developed to optimize the separation and detection of OTUs between 20 and 1,632 bp by using CE and laser-induced fluorescence detection. Additionally, we compared T-RFLP fingerprinting to DGGE optimized for detection of less abundant OTUs. Similar results were obtained with both fingerprinting techniques, although the T-RFLP approach and CE detection of OTUs was more sensitive, as indicated by the higher number of OTUs detected. We tested the T-RFLP fingerprinting technique on complex marine bacterial communities by using the 16S rRNA gene and 16S rRNA as templates for PCR. Samples from the Northern and Middle Adriatic Sea and from the South and North Aegean Sea were compared. Distinct clusters were identifiable for different sampling sites. Thus, this technique is useful for rapid evaluation of the biogeographical distribution and relationships of bacterioplankton communities.

Limiting Factors for Phytoplankton in the North Sea

1991 ◽  
Vol 24 (10) ◽  
pp. 261-267 ◽  
Author(s):  
J. C. H. Peeters ◽  
H. Haas ◽  
L. Peperzak ◽  
L. P. M. J. Wetsteyn
Keyword(s):  
North Sea ◽  
Coastal Waters ◽  
Light Attenuation ◽  
Limiting Factors ◽  
Surface Mixed Layer ◽  
Nutrient Loads ◽  
Nitrogen And Phosphorus ◽  
Attenuation Coefficients ◽  
The North ◽  
The North Sea

Underwater light intensity and nutrients are important for the abundance and species composition of phytoplankton. In winter the development of phytoplankton in the North Sea is limited by light. In the central parts of the North Sea the spring bloom starts earlier than in coastal areas. This difference is caused by the higher light attenuation coefficients in the coastal zone. In summer the surface mixed layer (SML) of the stratified areas shows light saturation of phytoplankton photosynthesis. During the growing season the continental coastal waters of the North Sea are potentially phosphorus or silicon limited when tested with a bioassay of the diatom Skeletonemacostatum. The offshore waters are potentially nitrogen limited. There are indications that the spring diatom bloom in the coastal waters ends because of silicon depletion and that the nitrogen and phosphorus concentrations are still large enough to allow the development of a large bloom of Phaeocvstiscf. pouchetii. In summer diatoms disappear from the SML of the stratified areas, but they survive under the thermocline. Possible effects of a reduction of the nutrient loads to the North Sea are discussed in the context of experience in the Delta region (SW-Netherlands).

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