Relative Influence of Plastic Debris Size and Shape, Chemical Composition and Phytoplankton-Bacteria Interactions in Driving Seawater Plastisphere Abundance, Diversity and Activity

The thin film of life that inhabits all plastics in the oceans, so-called “plastisphere,” has multiple effects on the fate and impacts of plastic in the marine environment. Here, we aimed to evaluate the relative influence of the plastic size, shape, chemical composition, and environmental changes such as a phytoplankton bloom in shaping the plastisphere abundance, diversity and activity. Polyethylene (PE) and polylactide acid (PLA) together with glass controls in the forms of meso-debris (18 mm diameter) and large-microplastics (LMP; 3 mm diameter), as well as small-microplastics (SMP) of 100 μm diameter with spherical or irregular shapes were immerged in seawater during 2 months. Results of bacterial abundance (confocal microscopy) and diversity (16S rRNA Illumina sequencing) indicated that the three classical biofilm colonization phases (primo-colonization after 3 days; growing phase after 10 days; maturation phase after 30 days) were not influenced by the size and the shape of the materials, even when a diatom bloom (Pseudo-nitzschia sp.) occurred after the first month of incubation. However, plastic size and shape had an effect on bacterial activity (3H leucine incorporation). Bacterial communities associated with the material of 100 μm size fraction showed the highest activity compared to all other material sizes. A mature biofilm developed within 30 days on all material types, with higher bacterial abundance on the plastics compared to glass, and distinct bacterial assemblages were detected on each material type. The diatom bloom event had a great impact on the plastisphere of all materials, resulting in a drastic change in diversity and activity. Our results showed that the plastic size and shape had relatively low influence on the plastisphere abundance, diversity, and activity, as compared to the plastic composition or the presence of a phytoplankton bloom.

The New Situation of Diatom Bloom in the Middle and Lower Reaches of Hanjiang River under River Chief System

In the middle and lower reaches of the Hanjiang River in February 2018, another serious diatom bloom occurred. The Changjiang Water Resources Commission promptly initiated an emergency plan to use the cascade reservoirs at the middle and lower reaches of the Hanjiang River to carry out water scheduling. At the same time, six monitoring sections were set up to daily monitor in the middle and lower reaches of the Hanjiang River. From 9 February to 18 March, this bloom had lasted more than 30 d. It is the longest since the record was recorded. The emergency dispatch measures adopted during the outbreak of the diatom bloom have stopped the further development of diatom bloom and played a good role. Analysing the causes of diatom bloom can provide a more comprehensive basis for further optimizing emergency dispatch. This paper analyzes the key hydrological factors that occur in this diatom bloom and proposes a water dispatching optimization plan and specific countermeasures. However, to permanently solve the diatom bloom problem, it is necessary for local environmental protection departments to work actively under the current requirements of the river chief system. Comprehensive protection of the Hanjiang River water ecological environment, completely solve the watershed problem of non-point source pollution.

Resilience of plankton trophic structure to an eddy-stimulated diatom bloom in the North Pacific Subtropical Gyre

We investigated the response of an open-ocean plankton food web to a major ecosystem perturbation event, the Hawaiian lee cyclonic eddy Opal, using compound-specific isotopic analyses of amino acids (CSIA-AA) of individual zooplankton taxa. We hypothesized that the massive diatom bloom that characterized Opal would lead to a shorter food chain. Using CSIA-AA, we differentiated trophic position (TP) changes that arose from altered transfers through protistan microzooplankton, versus metazoan carnivory, and assessed the variability at the base of the food web. Contrary to expectation, zooplankton TPs were higher in the eddy than in ambient control waters (up to 0.8 trophic level), particularly for suspension feeders close to the food-web base. Most of the effect was due to increased trophic transfers through protistan consumers, indicating a general shift up, not down, of grazing and remineralization in the microbial food web. Eucalanus sp., which was 15-fold more abundant inside compared to outside of the eddy, was the only taxon observed to be a true herbivore (TP = 2.0), consistent with a high phenylalanine (Phe) δ15N value indicating feeding on nitrate-fueled diatoms in the lower euphotic zone. Oncaea sp., an aggregate-associated copepod, had the largest (1.5) TP difference, and lowest Phe δ15N, suggesting that detrital particles were local hot spots of enhanced microbial activity. Rapid growth rates and trophic flexibility of protistan microzooplankton apparently allow the microbial community to reorganize to bloom perturbations, as microzooplankton remain the primary phytoplankton grazers—despite the dominance of large diatoms—and are heavily preyed on by the mesozooplankton.