scholarly journals Isolation of Elemental Sulfur as a Self-Growth-Inhibiting Substance Produced by Legionella pneumophila

2002 ◽  
Vol 68 (10) ◽  
pp. 4809-4811 ◽  
Author(s):  
Hiroaki Inoue ◽  
Genji Kawano ◽  
Hiromichi Nagasawa ◽  
Shohei Sakuda
Keyword(s):  
Legionella Pneumophila ◽  
Elemental Sulfur ◽  
Inhibiting Activity ◽  
Strong Growth ◽  
Methanol Extracts ◽  
Growth Inhibiting

ABSTRACT The addition of HP-20 resin to a medium could enhance the growth of Legionella species. Elemental sulfur was isolated as a self-growth-inhibiting substance produced endogenously by Legionella pneumophila from methanol extracts of the resins used to culture the bacterium. Elemental sulfur shows strong growth-inhibiting activity toward all Legionella species tested.

Inhibition of Estradiol-17ß-Induced Uterine Growth by Δ1, 9α Fluoro-17 Hydroxycorticosterone

1956 ◽  
Vol 186 (3) ◽  
pp. 468-470 ◽  
Author(s):  
Joseph T. Velardo
Keyword(s):  
Double Bond ◽  
Ovariectomized Rat ◽  
Albino Rats ◽  
Inhibiting Activity ◽  
Uterine Weight ◽  
Uterine Growth ◽  
Growth Inhibiting

Adult, albino rats of the Charles River strain, 100 days of age, weighing 190–210 gm, were bilaterally ovariectomized, and 1 week later were placed on experiments to ascertain the influence of Δ1, 9α fluoro-17-hydroxycorticosterone (ΔFF) on estradiol-17ß-induced uterine growth. The results indicate that ΔFF when administered in a daily dosage up to 0.20 mg for 3 days did not modify the weight of the uterus of the ovariectomized rat, whereas 3 daily dosages of 0.10 µg estradiol-17ß effected an increase of approximately 85% in uterine weight. When 0.05–0.20 mg ΔFF was injected daily, but at different sites, with 0.10 µg estradiol-17ß for the 3-day period, the response of the uterus to estradiol-17ß was markedly reduced from the estradiol-17ß-induced increase of 85% to 52% on the lowest dosage of ΔFF down to 24% on the highest dose (0.20 mg) of ΔFF. These experiments further indicate that the inhibition of estradiol-17ß-induced uterine growth could be partially reversed by increasing the dosage of estradiol-17ß. Comparatively, data at hand suggest that ΔFF > 9αFlF > compound F > compound E > Δ1E and Δ1F in inhibiting 0.10 µg estradiol-17ß on the uterus and the vagina of the ovariectomized rat. Moreover, the incorporation of an additional double bond in ring A (Δ1) and flourine atom in the 9α position of compound F enhances the uterine growth inhibiting activity of compound F. Of the numerous glucocorticoids and mineralocorticoids tested for ability to inhibit estradiol-17ß, ΔFF is by far the most efficacious.

scholarly journals Draft Genome Sequences of Three Sponge-Associated Actinomycetes Exhibiting Antimycobacterial Activity

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Daniela Tizabi ◽  
Ana Sosa ◽  
Tsvetan Bachvaroff ◽  
Russell T. Hill
Keyword(s):  
Draft Genome ◽  
Antimycobacterial Activity ◽  
Inhibiting Activity ◽  
Marine Actinomycetes ◽  
Genome Sequences ◽  
Content Type ◽  
Promising Source ◽  
Growth Inhibiting

Marine actinomycetes (order Actinomycetales) are of interest as a promising source of pharmaceuticals. The genomes of three novel sponge-associated actinomycetes exhibiting antimycobacterial activity, Brevibacterium sp. strain XM4083, Micrococcus sp. strain R8502A1, and Micromonospora sp. strain XM-20-01, were sequenced in an effort to identify compounds responsible for growth-inhibiting activity.

scholarly journals Gamma interferon is the lymphokine and beta interferon the monokine responsible for inhibition of fibroblast collagen production and late but not early fibroblast proliferation.

1985 ◽  
Vol 162 (2) ◽  
pp. 516-527 ◽  
Author(s):  
M R Duncan ◽  
B Berman
Keyword(s):  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Gamma Interferon ◽  
Dermal Fibroblasts ◽  
Inhibiting Activity ◽  
Con A ◽  
Ifn Gamma ◽  
Collagen Production ◽  
Ifn Alpha ◽  
Growth Inhibiting

Human peripheral blood mononuclear cells activated with concanavalin A (Con A) or lipopolysaccharide (LPS) produce, respectively, lymphokines (LK) of 50,000 Mr or monokines (MK) of 20,000 Mr that inhibit the growth and collagen production of cultured human dermal fibroblasts. Because antigenic typing of the antiviral activity of these LK and MK preparations revealed that LK contained mainly gamma interferon (IFN-gamma), and MK, primarily IFN-beta, we investigated if any of the fibroblast-inhibiting activities could be attributed to human IFN. Unlike LK and MK, which act within 24 h to inhibit the growth of subconfluent foreskin and adult dermal fibroblasts, samples of purified, natural derived IFN-alpha, -beta, and -gamma and recombinant DNA-derived IFN-alpha 2 and -gamma were ineffective inhibitors at 24 h and required 48-72 h to significantly inhibit growth. However, all IFN did mimic LK/MK action in causing concentration-dependent inhibition of collagen production by confluent fibroblast microcultures. Furthermore, the collagen production-inhibiting activity of Con A-induced LK supernatant and its 50,000 Mr fraction was completely suppressed by 10(3) neutralizing U/ml of either polyclonal or monoclonal antibody to IFN-gamma, while polyclonal antibodies to IFN-alpha and -beta had no effect. Similarly, the collagen production-inhibiting activity of LPS-induced MK supernatant and its 20,000 Mr fraction was suppressed by polyclonal anti-IFN-beta but not by anti-IFN-alpha or -gamma. Anti-IFN failed to reverse early-acting LK or MK growth-inhibiting activities. These data suggest collagen production-inhibiting LK and MK are IFN-gamma and IFN-beta, respectively, and that early acting, growth-inhibiting LK and MK are not IFN.

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