Preparation of an active recombinant peptide of crustacean androgenic gland hormone 1 1Abbreviations: AG: androgenic gland; AGH: androgenic gland hormone; Bac-rAGH: baculovirus-recombinant androgenic gland hormone; E. coli-rAGH: Escherichia coli-recombinant androgenic gland hormone; HPLC: high-performance liquid chromatography (HPLC); PCR: PCR; PTH: phenylthiohydantoin; TFA: trifluoroacetic acid; TOF: time-of-flight.

Peptides ◽  
2002 ◽  
Vol 23 (3) ◽  
pp. 567-572 ◽  
Author(s):  
Atsuro Okuno ◽  
Yuriko Hasegawa ◽  
Makoto Nishiyama ◽  
Tsuyoshi Ohira ◽  
Rinkei Ko ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Trifluoroacetic Acid ◽  
High Performance ◽  
Time Of Flight ◽  
Androgenic Gland ◽  
Recombinant Peptide ◽  
E Coli

scholarly journals Production of Sepiapterin in Escherichia coli by Coexpression of Cyanobacterial GTP Cyclohydrolase I and Human 6-Pyruvoyltetrahydropterin Synthase

2002 ◽  
Vol 68 (6) ◽  
pp. 3138-3140 ◽  
Author(s):  
Hyun Joo Woo ◽  
Jee Yun Kang ◽  
Yong Kee Choi ◽  
Young Shik Park
Keyword(s):  
Escherichia Coli ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aldose Reductase ◽  
High Performance ◽  
Gtp Cyclohydrolase I ◽  
Gtp Cyclohydrolase ◽  
E Coli ◽  
Sepiapterin Reductase ◽  
Pcc 6803

ABSTRACT Synechocystis sp. strain PCC 6803 GTP cyclohydrolase I and human 6-pyruvoyltetrahydropterin synthase were coexpressed in Escherichia coli. The E. coli transformant produced sepiapterin, which was identified by high-performance liquid chromatography and enzymatically converted to dihydrobiopterin by sepiapterin reductase. Aldose reductase, another indispensable enzyme for sepiapterin production, may be endogenous in E. coli.

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