scholarly journals A Rapid Microtiter Plate Method To Measure Carbon Dioxide Evolved from Carbon Substrate Amendments so as To Determine the Physiological Profiles of Soil Microbial Communities by Using Whole Soil

2003 ◽  
Vol 69 (6) ◽  
pp. 3593-3599 ◽  
Author(s):  
Colin D. Campbell ◽  
Stephen J. Chapman ◽  
Clare M. Cameron ◽  
Mitchell S. Davidson ◽  
Jacqueline M. Potts
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Microtiter Plate ◽  
Wastewater Sludge ◽  
Carbon Substrate ◽  
Soil Microbial ◽  
Deep Well

ABSTRACT Sole-carbon-source tests (Biolog), designed to identify bacteria, have become very popular for metabolically fingerprinting soil microbial communities, despite disadvantages associated with the use of carbon source profiles that primarily select for fast-growing bacteria. In this paper we describe the use of an alternative method that combines the advantages of the Biolog community-level physiological profile (CLPP) method, in which microtiter-based detection plates are used, with the ability to measure carbon dioxide evolution from whole soil. This method facilitates measurement over short periods of time (4 to 6 h) and does not require the extraction and culturing of organisms. Deep-well microtiter plates are used as test wells into which soil is placed. The apparatus to fill the deep-well plates and interface it with a second removable detection plate is described. Two detection systems, a simple colorimetric reaction in absorbent alkali and scintillation counting with radioactive carbon sources, are described. The methods were compared to the Biolog-CLPP system by using soils under different vegetation types and soil treated with wastewater sludge. We aimed to test the hypothesis that using whole soil would have specific advantages over using extracts in that more immediate responses to substrates could be obtained that would reflect activity rather than growth. The whole-soil method was more rapid and gave earlier detection of C source use. Also, the metabolic fingerprints obtained could discriminate between sludge treatments.

Utilization of carbon sources by the soil microbial communities of different forest types in subtropical Australia

2012 ◽  
Vol 32 (9) ◽  
pp. 2819-2826
Author(s):  
鲁顺保 LU Shunbao ◽  
郭晓敏 GUO Xiaomin ◽  
芮亦超 RUI Yichao ◽  
周小奇 ZHOU Xiaoqi ◽  
陈成榕 CHEN Chengrong ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Forest Types ◽  
Soil Microbial ◽  
Subtropical Australia

Soil Microbial Communities Involved in Reductive Dissolution of Arsenic from Arsenate-Laden Minerals with Different Carbon Sources

2019 ◽  
Vol 53 (21) ◽  
pp. 12398-12406 ◽  
Author(s):  
Shigeki Yamamura ◽  
Hibiki Kurasawa ◽  
Yuta Kashiwabara ◽  
Tomoyuki Hori ◽  
Tomo Aoyagi ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Reductive Dissolution ◽  
Soil Microbial

scholarly journals Patterns in utilization of carbon sources in soil microbial communities contaminated with mine solid wastes from San Luis Potosi, Mexico

2021 ◽  
Vol 208 ◽  
pp. 111493 ◽  
Author(s):  
Ángeles Martínez-Toledo ◽  
Donaji J. González-Mille ◽  
Maria E. García-Arreola ◽  
Omar Cruz-Santiago ◽  
Antonio Trejo-Acevedo ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Solid Wastes ◽  
Soil Microbial ◽  
San Luis ◽  
San Luis Potosi ◽  
San Luis Potosí

scholarly journals Elevated carbon dioxide accelerates the spatial turnover of soil microbial communities

2015 ◽  
Vol 22 (2) ◽  
pp. 957-964 ◽  
Author(s):  
Ye Deng ◽  
Zhili He ◽  
Jinbo Xiong ◽  
Hao Yu ◽  
Meiying Xu ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Microbial Communities ◽  
Soil Microbial Communities ◽  
Elevated Carbon Dioxide ◽  
Soil Microbial ◽  
Spatial Turnover

scholarly journals Microbial communities and their predictive functional profiles in the arid soil of Saudi Arabia

SOIL ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 513-521
Author(s):  
Munawwar A. Khan ◽  
Shams T. Khan
Keyword(s):  
Saudi Arabia ◽  
Microbial Communities ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Survival Strategies ◽  
Semiarid Region ◽  
Ammonia Oxidizers ◽  
Arid Soil ◽  
Soil Microbial ◽  
Nitrogen Fixers

Abstract. Saudi Arabia has the world's fifth-largest desert and is the biggest importer of food and agricultural products. Understanding soil microbial communities is key to improving the agricultural potential of the region. Therefore, soil microbial communities of the semiarid region of Abha, known for agriculture, and arid regions of Hafar Al Batin and Muzahmiya were studied using Illumina sequencing. The results show that the microbial communities of the Saudi desert were characterized by the presence of high numbers of Actinobacteria, Proteobacteria, and Firmicutes. In addition to Sahara desert signature phyla like Gemmatimonadetes, biogeochemically important microorganisms like primary producers, nitrogen fixers and ammonia oxidizers were also present. The composition of the microbial community varied greatly among the sites sampled. The highest diversity was found in the rhizospheric soil of Muzahmiya followed by Abha. Firmicutes, Proteobacteria and Actinobacteria were the three main phyla detected in all the samples. Soils from the agricultural region of Abha were significantly different from other samples in containing only 1 % Firmicutes and 3–6 times higher population of Actinobacteria and Bacteroidetes, respectively. The presence of photosynthetic bacteria, ammonia oxidizers, and nitrogen fixers along with bacteria capable of surviving on simple and unlikely carbon sources like dimethylformamide was indicative of their survival strategies under harsh environmental conditions in the arid soil. Functional inference using PICRUSt analysis shows an abundance of genes involved in photosynthesis and nitrogen fixation.

scholarly journals Unravelling the Functional Diversity of the Soil Microbial Community of Chinese Fir Plantations of Different Densities

Forests ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 532 ◽  
Author(s):  
Chaoqun Wang ◽  
Lin Xue ◽  
Yuhong Dong ◽  
Yihui Wei ◽  
Ruzhen Jiao
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Functional Diversity ◽  
Soil Microbial Community ◽  
Diversity Index ◽  
Carbon Sources ◽  
Soil Microbial Communities ◽  
Stand Density ◽  
Chinese Fir ◽  
Soil Microbial

The structure and function of forest ecosystems are directly or indirectly affected by their stand density. However, what effect the density of Chinese fir plantations has on the functional diversity of the soil microbial community remains unclear. The microbial metabolic functional diversity of soils sampled at the topsoil (0–20 cm) of 35-year-old Chinese fir plantations of five initial densities (D1: 1667 stems∙hm−2, D2: 3333 stems∙hm−2, D3: 5000 stems∙hm−2, D4: 6667 stems∙hm−2, and D5: 10,000 stems∙hm−2) was studied by using Biolog ECO technology. The results showed that the soil pH, oxidizable organic carbon (SOOC), available N (AN), available P (AP), and available K (AK) contents all showed a gradual increase from D1 to D4 and a decrease from D4 to D5, while the number of culturable bacteria and total microorganisms, the average well color development (AWCD) values for the single carbon substrate and six types of carbon sources used by the microbial community, as well as the Shannon-Wiener diversity index (H’), Pielou evenness index (J), and McIntosh Diversity Index (U), were the opposite, suggesting that low-densities favored C and N mineralization and the nutrient cycle. The density of Chinese fir plantations had a significant effect on the use of carbohydrates, amino acids, carboxylic acids, and phenolic acids by the soil microbial community, but it had no significant effect on the use of polymers (p < 0.05). Principal component analysis (PCA) revealed that carbohydrates, polymers, and phenolic acids were sensitive carbon sources that caused differences in the metabolic functions of soil microbial communities in Chinese fir plantations. Redundancy analysis (RDA) showed that physicochemical factors have a significant influence on the metabolic function of soil microbial communities (RDA1 and RDA2 explained >85% variance). The changes in density affected the soil physicochemical properties, the composition, and the metabolic functional diversity of microbial communities in Chinese fir plantations, which is certainly useful for the stand density regulation of Chinese fir plantations.

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