scholarly journals Evaluation of Enzyme-Linked Immunosorbent Assays for Detection of Mycoplasma bovis-Specific Antibody in Bison Sera

2013 ◽  
Vol 20 (9) ◽  
pp. 1405-1409 ◽  
Author(s):  
Karen B. Register ◽  
Randy E. Sacco ◽  
Steven C. Olsen
Keyword(s):  
Infectious Disease ◽  
Protein G ◽  
Mycoplasma Bovis ◽  
Content Type ◽  
Specific Serum ◽  
History Of ◽  
Specific Igg ◽  
Immunosorbent Assays ◽  
Comparison Of The Results ◽  
Antibody Levels

ABSTRACTMycoplasma bovishas recently emerged as a significant and costly infectious disease problem in bison. A method for the detection ofM. bovis-specific serum antibodies is needed in order to establish prevalence and transmission patterns. Enzyme-linked immunosorbent assays (ELISAs) validated for the detection ofM. bovis-specific serum IgG in cattle are commercially available, but their suitability for bison sera has not been determined. A collection of bison sera, most from animals with a known history of infection or vaccination withM. bovis, was tested forM. bovis-specific IgG using commercially available kits as well as an in-house ELISA in which either cattle or bisonM. bovisisolates were used as a source of antigen. Comparison of the results demonstrates that ELISAs optimized for cattle sera may not be optimal for the identification of bison seropositive forM. bovis, particularly those with low to moderate antibody levels. The reagent used for the detection of bison IgG and the source of the antigen affect the sensitivity of the assay. Optimal performance was obtained when the capture antigen was derived from bison isolates rather than cattle isolates and when a protein G conjugate rather than an anti-bovine IgG conjugate was used for the detection of bison IgG.

scholarly journals Development of a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Mycoplasma bovis Infection in Cattle

2013 ◽  
Vol 21 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Nadeeka K. Wawegama ◽  
Glenn F. Browning ◽  
Anna Kanci ◽  
Marc S. Marenda ◽  
Philip F. Markham
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Mycoplasma Bovis ◽  
Western Blots ◽  
Content Type ◽  
Amino Terminal ◽  
Igm Elisa ◽  
Specific Igg ◽  
Lipase A ◽  
Strong Immunoreactivity ◽  
Gst Fusion Proteins

ABSTRACTMycoplasma boviscauses a range of diseases in cattle, including mastitis, arthritis, and pneumonia. However, accurate serological diagnosis of infection remains problematic. The studies described here aimed to identify an antigen that might be used to develop a more specific and sensitive diagnostic assay. A 226-kDa immunogenic protein was consistently detected in Western blots by antibodies in sera from calves experimentally infected withM. bovis. This protein was shown to be a membrane protein with lipase activity and was named mycoplasma immunogenic lipase A (MilA). Different regions of MilA were expressed inEscherichia colias glutathioneS-transferase (GST) fusion proteins and recombinant products from the amino-terminal end shown to have strong immunoreactivity withM. bovis-specific bovine sera. The most immunoreactive fusion protein, GST-MilA-ab, was used to develop indirect IgM and IgG enzyme-linked immunosorbent assays (ELISAs). The IgM ELISA detectedM. bovis-specific IgM antibody 2 weeks after infection with 97.1% sensitivity and had a specificity of 63.3%, while the IgG ELISA detectedM. bovis-specific IgG 3 weeks after infection with 92.86% sensitivity and had a specificity of 98.7%, demonstrating that the IgG ELISA has potential for use as a sensitive and specific assay for detecting infection in cattle.

THE CONTENT OF ANTIBODIES TO THE SARS-COV-2 VIRUS IN STUDENTS OF A HIGHER EDUCATIONAL INSTITUTION

2021 ◽  
Author(s):  
V.T. Akhmetshina ◽  
◽  
L.G. Gizatullina ◽  
L.M. Masyagutova
Keyword(s):  
Educational Institution ◽  
Dynamic Monitoring ◽  
Igg Antibodies ◽  
Average Value ◽  
Population Immunity ◽  
History Of ◽  
Specific Igg ◽  
Higher Educational ◽  
Antibody Levels ◽  
Specific Igg Antibodies

Abstract: Abstract: Today, a request is being formed to prevent the introduction of infection into organized groups by means of the formation of population immunity by methods of specific prevention. Purpose of the work: To carry out the determination of specific IgG antibodies to SARS-CoV-2 in students of a higher educational institution, to determine the number of students in need of vaccination. Material and research methods: The level of IgG antibodies to SARS-CoV-2 in the blood serum of students of a higher educational institution was analyzed. An analysis of the strength of immunity shows that the average value of the CP of positive samples is 11.3. A more significant diagnostic level of CP was revealed, indicating a pronounced tension of immunity in students with a history of pneumonia. Among those with a diagnostically significant positive CP level, more than a third of the examined subjects have lower IgG antibodies to SARS-CoV-2 than the average in this group. Consequently, these individuals require dynamic observation and monitoring of antibody levels in order to ensure timely vaccination. Thus, specific IgG antibodies to SARS-CoV-2 were detected in half of the examined students, which was 55%. It is this group that is subject to immediate vaccination before undergoing industrial practice. A third of students with low levels of antibodies to SARS-CoV-2 need dynamic monitoring of their content.

scholarly journals Enzyme-linked immunosorbent assays for the measurement of specific antibodies in experimentally induced ovine toxoplasmosis

1988 ◽  
Vol 100 (2) ◽  
pp. 205-212 ◽  
Author(s):  
R. A. Payne ◽  
D. H. M. Joynson ◽  
A. J. Wilsmore
Keyword(s):  
Toxoplasma Gondii ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Capture Assay ◽  
Previous Infection ◽  
Specific Igg ◽  
Immunosorbent Assays ◽  
Antibody Class ◽  
Antibody Levels ◽  
Indirect Assay

SUMMARYTachyzoitcs of the RH strain of Toxoplasma gondii were inoculated intravenously into sheep following which serum samples were collected at approximately weekly intervals for 9 months. The sera were examined by the toxoplasma dye test and two enzymc-linkcd immunosorbent assays (ELISA) specifically developed for investigations of ovine toxoplasmosis. One was an antibody class capture assay for the detection of anti-toxoplasma specific IgM, the other an indirect assay which detected anti-toxoplasma IgG.Some of the sheep had antibodies to toxoplasma prior to inoculation but none had specific IgM. Sera collected 17 days after inoculation showed that all had raised specific antibody levels but the only sheep that produced specific antitoxoplasma IgM were those that were initially without any antibody. Specific IgM could be detected in all these particular sheep for at least 1 month after infection and up to 3 months in some. Specific IgG persisted at high levels for at least 3 months and could still be detected at moderate levels for at least 9 months. The ELISA methods described are simple to perform and could clearly distinguish between previous infection and this experimental infection with Toxoplasma gondii.

scholarly journals Functional and Antigen-Specific Serum Antibody Levels as Correlates of Protection against Shigellosis in a Controlled Human Challenge Study

2016 ◽  
Vol 24 (2) ◽  
Author(s):  
Avital A. Shimanovich ◽  
Amanda D. Buskirk ◽  
Shannon J. Heine ◽  
William C. Blackwelder ◽  
Rezwanul Wahid ◽  
...  
Keyword(s):  
Diarrheal Disease ◽  
Shigella Flexneri ◽  
Serum Antibody ◽  
Oral Immunization ◽  
Live Attenuated Vaccine ◽  
Content Type ◽  
Vaccine Candidates ◽  
Correlates Of Protection ◽  
Specific Serum ◽  
Specific Igg

ABSTRACT Shigella is an important cause of diarrheal disease in young children living in developing countries. No approved vaccines are available, and the development of vaccine candidates has been hindered by the lack of firm immunological correlates of protection, among other reasons. To address this gap in knowledge, we established quantitative assays to measure Shigella-specific serum bactericidal antibody (SBA) and opsonophagocytic killing antibody (OPKA) activities and investigated their potential association with protection against disease in humans. SBA, OPKA, and Ipa-, VirG (IscA)-, and Shigella flexneri 2a lipopolysaccharide-specific serum IgG titers were determined in adult volunteers who received Shigella vaccine candidate EcSf2a-2 and in unvaccinated controls, all of whom were challenged with virulent Shigella flexneri 2a. Prechallenge antibody titers were compared with disease severity after challenge. SBA and OPKA, as well as IpaB- and VirG-specific IgG, significantly correlated with reduced illness. SBA and OPKA assays were also used to evaluate the immunogenicity of leading live attenuated vaccine candidates Shigella CVD 1204 and CVD 1208S in humans. A single oral immunization with CVD 1204 or CVD 1208S resulted in SBA seroconversion rates of 71% and 47% and OPKA seroconversion rates of 57% and 35%, respectively. Higher functional antibody responses were induced by CVD 1204, which is consistent with its lower attenuation. This is the first demonstration of SBA, OPKA, and IpaB- and VirG-specific IgG levels as potential serological correlates of protection against shigellosis in humans. These results warrant further studies to establish their capacity to predict protective immunity and vaccine efficacy.

scholarly journals Neisseria gonorrhoeae NspA Induces Specific Bactericidal and Opsonic Antibodies in Mice

2011 ◽  
Vol 18 (11) ◽  
pp. 1817-1822 ◽  
Author(s):  
Guocai Li ◽  
Hongmei Jiao ◽  
Guihua Jiang ◽  
Jing Wang ◽  
Litian Zhu ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Vaccine Development ◽  
Protein A ◽  
Surface Protein ◽  
Secretory Iga ◽  
Dna Immunization ◽  
Content Type ◽  
Specific Igg ◽  
Immunosorbent Assays ◽  
Vaginal Wash

ABSTRACTNeisseria gonorrhoeaesurface protein A (NspA) is a highly conserved gonococcal antigen. To explore the potential of NspA in vaccine development against gonorrhea, BALB/c mice were immunized with pcNspA containing the NspA gene fromN. gonorrhoeaestrain WHO-A via intramuscular (i.m.) injection, intranasal (i.n.) immunization, or intravaginal (i.vag.) immunization. Following the last DNA immunization, mice were boosted with recombinant NspA (rNspA). Enzyme-linked immunosorbent assays (ELISAs) indicated that all immunized mice generated measurable NspA-specific IgG and IgA in serum and secretory IgA (sIgA) in vaginal wash fluids. The antisera had bactericidal and opsonic activities. These data demonstrated that NspA induced antibodies with antigonococcal activity.

scholarly journals Evaluation of Anticoagulants for Serologic Assays of Cholera Vaccination

2014 ◽  
Vol 21 (6) ◽  
pp. 854-858 ◽  
Author(s):  
Jae Seung Yang ◽  
Seok-Seong Kang ◽  
Cheol-Heui Yun ◽  
Seung Hyun Han
Keyword(s):  
Pearson Correlation ◽  
Plasma Samples ◽  
Content Type ◽  
Simultaneous Evaluation ◽  
Antibody Titers ◽  
Acid Citrate Dextrose ◽  
Specific Igg ◽  
Citrate Dextrose ◽  
Cholera Vaccination ◽  
Antibody Levels

ABSTRACTBlood collected with an anticoagulant is beneficial for simultaneous evaluation of immune cells and humoral components such as antibodies. However, it is critical that the anticoagulant does not affect quantitative and qualitative analyses of antibodies. In the present study, we examined the potential interference of the widely used anticoagulants heparin, EDTA, and acid citrate dextrose (ACD) on vibriocidal antibody activities andVibrio choleraelipopolysaccharide (LPS)-specific IgG, IgM, and IgA levels in the plasma and sera obtained from cholera patients or vaccinees. Serum vibriocidal antibody titer was inhibited in the presence of EDTA or ACD but not in the presence of heparin. Moreover, 100% (8/8) of the vibriocidal antibody titers of plasma samples obtained from the vaccinees in tubes containing heparin were identical to the titers of matched sera when compared with 37.5% (3/8) and 50% (4/8) of the plasma samples prepared with EDTA and ACD, respectively. Among LPS-specific Igs, the Pearson correlation coefficient (r) for IgA in serum and plasma was low (r= 0.716), and the coefficients for IgG and IgM were relatively high (r= 0.997 andr= 0.945, respectively) in heparinized plasma samples compared with the coefficient for IgG and IgM of EDTA- and ACD-treated plasma. Our results suggest that heparin is an appropriate anticoagulant for the collection of blood when measuring vibriocidal activities and antibody levels in plasma samples.

scholarly journals 570. “I don’t know”: The Typical Response When Taking Pneumococcal Immunization Histories in Kidney Transplant Candidates

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S349-S351
Author(s):  
Jahanavi M Ramakrishna ◽  
Tambi Jarmi ◽  
Claudia R Libertin
Keyword(s):  
Infectious Disease ◽  
Kidney Transplant ◽  
Pneumococcal Vaccine ◽  
Patient Population ◽  
Pneumococcal Vaccination ◽  
Prior History ◽  
Vaccine Preventable Diseases ◽  
Number Of Patients ◽  
Order Completion ◽  
History Of

Abstract Background Vaccine-preventable diseases account for significant morbidity and mortality in the kidney transplant (KT) patient population. AST Guidelines support review and documentation of pneumococcal vaccines in KT candidate infectious disease (ID) evaluations. The objective of this study is to determine the number of KT candidates screened for prior pneumococcal immunizations and the frequency of vaccines ordered by providers when indicated at Mayo Clinic Florida’s (MCF) Transplant Center. Methods This study was an institution-based retrospective analysis of all KT candidates evaluated at MCF from December 2, 2019 – January 14, 2020. Data collection was obtained by electronic health record review. Outcomes included known history and documentation rates of prior pneumococcal vaccinations (both Prevnar 13 and Pneumovax 23) by infectious disease (ID) providers, as well as pneumococcal vaccine order frequency during ID pre-transplant evaluation when indicated. Data analysis was done using simple descriptive statistics. Results Sixty-one patients underwent KT evaluation during the study period. Among the 61 patients, 20 (32.8%) and 20 (32.8%) had a known prior history of receiving Prevnar 13 and Pneumovax 23 vaccinations, respectively. Vaccine history was unknown for Prevnar 13 and Pneumovax 23 in 39 (63.9%) patients. Vaccine status was not documented by ID providers in 2 (3.3%) patients. When appropriate, ID providers ordered Prevnar 13 and Pneumovax 23 in 38 (92.7%) and 41 (100%) patients, respectively. Orders included both electronic and written documentation to account for patients planning immunization elsewhere. Of the 38 patients advised to receive the Prevnar 13 vaccine, 17 (41.5%) patients were documented completing immunization. Pneumovax 23 order completion rates were not recorded since the study period only lasted six weeks due to closure by COVID-19. Table 1. Pneumococcal Vaccine History Documentation Rates Obtained by Patient Recall or Records Table 2. Pneumococcal Vaccine Order Rates at Pre-Kidney Transplant Consultations Table 3. Prevnar 13 Order Completion Rate by Documentation Conclusion The data reflect a high number of patients who either do not recall or have documentation of prior pneumococcal vaccination available at time of KT ID evaluation. Providers documented history of pneumococcal vaccinations extremely well, ordering immunizations when necessary. This study highlights lack of portability of immunization histories in a given patient population and opportunity for improved care. Disclosures Claudia R. Libertin, MD, Pfizer, Inc. (Grant/Research Support, Research Grant or Support)

A tribute to Gwen Leighty

2006 ◽  
Vol 19 (2) ◽  
pp. 84-86
Author(s):  
Jennifer Paustenbaugh
Keyword(s):  
Web Sites ◽  
Academic Libraries ◽  
Design Methodology ◽  
Fund Raising ◽  
Personal Knowledge ◽  
Valuable Contribution ◽  
Content Type ◽  
History Of ◽  
Development Network ◽  
Fund Raiser

PurposeThe purpose of the paper is to provide a tribute to the life and work of library fund‐raiser Gwen Leighty.Design/methodology/approachThe paper uses personal knowledge and references to Academic Libraries Advancement and Development Network (ALADN) and LIBDEV web sites.FindingsThe paper finds that fundraising is connecting with people and the journey that each development officer must make while raising funds for their library.Originality/valueThe paper presents a brief history of ALADN and the valuable contribution one person made to the cause of library fund‐raising.

The sediment of the Hostivar Reservoir (Prague, Czech Republic) as a memory of 45 years of pollution

2017 ◽  
Vol 28 (2) ◽  
pp. 204-213
Author(s):  
Lucie Soucková ◽  
Dana Kominkova
Keyword(s):  
Czech Republic ◽  
Design Methodology ◽  
Polyaromatic Hydrocarbons ◽  
Reservoir Sediment ◽  
Terrestrial Environment ◽  
Content Type ◽  
Sediment Extraction ◽  
History Of ◽  
Us Epa ◽  
Practical Implications

Purpose The purpose of this paper is to evaluate the historical pollution of the Hostivar Reservoir (largest reservoir in Prague) sediment by metals, polyaromatic hydrocarbons (PAH) and polychlorinated biphenyls (PCB) and identify the trends in pollution of aquatic environment. Design/methodology/approach Core samples, 140 cm long, recording the 45-year history of the reservoir, were separated to 5 cm width subsamples (approximately 1.5 years of sedimentation) and analyzed for metals (Cd, Pb, Cu, Zn, Cr, Ni, Al), PAH and PCB. Following methods were used: US EPA 3051 for metals, US EPA 505 and US EPA 8082 A for PCB, and ISO 18287:2006 for PAH. Findings Most of the contaminants had the highest concentration at the beginning of the existence of the reservoir, suggesting that the contamination results from construction activities. Significant decrease of Pb occurred in the second half of the 1990s. It was caused by termination of the addition of lead as a detonation suppressant to the gasoline. Most concentrations of PAHs, PCBs and metals, except copper do not present eco-toxicological risk. Practical implications The results show the volume of priority pollutants removed from the reservoir by sediment extraction, and point risk to the terrestrial environment due to application of the sediment in the construction of a noise protecting wall. Originality/value The paper presents unique data about historical contamination of the largest reservoir in Prague, the capital of Czech Republic. It shows how the watershed and the construction phase of the dam cause a pollution of the reservoir sediment and possible environmental risk for aquatic biota.

A brief history of small business in Australia, 1970-2010

2014 ◽  
Vol 3 (2) ◽  
pp. 222-236 ◽  
Author(s):  
Michael T. Schaper
Keyword(s):  
Small Business ◽  
Small Firms ◽  
Formal Education ◽  
Extensive Literature ◽  
Business Sector ◽  
Role Of Government ◽  
Content Type ◽  
Support Tools ◽  
History Of ◽  
Training And Research

Purpose – The purpose of this paper is to provide an overview of the development of the SME sector in Australia, concentrating on a number of key areas: small business definitions and numbers; the role of government; the emergence of key industry groups; and the evolution of education, training and research services. Design/methodology/approach – The study is a result of extensive literature reviews, desk research and the recollections of various participants in the field. Findings – There have been major changes to the Australian small business sector over the last 40 years. In 1983-1984 there were an estimated 550,000 small firms, and by 2010 this had grown to almost two million. Government involvement in, and support for, SMEs was virtually non-existent before 1970. Following the delivery of the Wiltshire report (1971), however, both state and federal governments responded by developing specialist advisory services, funding programmes and other support tools. Virtually non-existent before the 1970s, several peak industry associations were formed between 1977 and the 1990s. At the same time, formal education and teaching in the area expanded in the 1970s and 1980s and is now widespread. Practical implications – Development of the small business sector in Australia has often paralleled similar trends in other OECD nations. State and territory governments have often (but not always) been the principal drivers of policy change. Originality/value – There has been no little, if any, prior documentation of the evolution of the small business sector in Australia in the last 40 years.

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