scholarly journals Genome Sequence of a Clinical Strain of Acinetobacter baumannii Belonging to the ST79/PFGE-HUI-1 Clone Lacking the AdeABC (Resistance-Nodulation-Cell Division-Type) Efflux Pump

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
M. López ◽  
L. Álvarez-Fraga ◽  
E. Gato ◽  
L. Blasco ◽  
M. Poza ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cell Division ◽  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Efflux Pump ◽  
Clinical Strain ◽  
Genetic Characteristics ◽  
Clinical Strains ◽  
Chromosomal Genes

Increased expression of chromosomal genes for resistance-nodulation-cell division-type efflux systems plays a major role in the multidrug resistance of Acinetobacter baumannii . Little is known about the genetic characteristics of clinical strains of Acinetobacter baumannii lacking the AdeABC pump. In this study, we sequenced the genome of clinical strain Ab421 GEIH-2010 (belonging to clone ST79/PFGE-HUI-1 from the GEIH-REIPI Ab. 2010 project) which lacks this efflux pump.

scholarly journals First Description of an RND-Type Multidrug Efflux Pump in Achromobacter xylosoxidans, AxyABM

2011 ◽  
Vol 55 (10) ◽  
pp. 4912-4914 ◽  
Author(s):  
Julien Bador ◽  
Lucie Amoureux ◽  
Jean-Marie Duez ◽  
Anthony Drabowicz ◽  
Eliane Siebor ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Multidrug Resistance ◽  
Cell Division ◽  
Efflux Pump ◽  
Achromobacter Xylosoxidans ◽  
Clinical Strain ◽  
Multidrug Efflux ◽  
Emerging Pathogen ◽  
Multidrug Efflux Pump ◽  
Content Type

ABSTRACTAchromobacter xylosoxidansis an emerging pathogen in cystic fibrosis patients. The multidrug resistance of these bacteria remains poorly understood. We have characterized in a clinical strain the first resistance-nodulation-cell division (RND)-type multidrug efflux pump in this species: AxyABM. The inactivation of the transporter componentaxyBgene led to decreased MICs of cephalosporins (except cefepime), aztreonam, nalidixic acid, fluoroquinolones, and chloramphenicol.

scholarly journals Draft Genome Sequence of the Biofilm-Hyperproducing Acinetobacter baumannii Clinical Strain MAR002

2015 ◽  
Vol 3 (4) ◽  
Author(s):  
Laura Álvarez-Fraga ◽  
María López ◽  
María Merino ◽  
Soraya Rumbo-Feal ◽  
María Tomás ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Clinical Strain

scholarly journals First Complete Providencia rettgeri Genome Sequence, the NDM-1-Producing Clinical Strain RB151

2017 ◽  
Vol 5 (3) ◽  
Author(s):  
R. Alejandro Marquez-Ortiz ◽  
Leanne Haggerty ◽  
Eby M. Sim ◽  
Carolina Duarte ◽  
Betsy E. Castro-Cardozo ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Bacterial Pathogen ◽  
Clinical Strain ◽  
Providencia Rettgeri ◽  
Content Type ◽  
Tract Infections

ABSTRACT Providencia rettgeri is an opportunistic bacterial pathogen of clinical significance due to its association with urinary tract infections and multidrug resistance. Here, we report the first complete genome sequence of P. rettgeri. The genome of strain RB151 consists of a 4.8-Mbp chromosome and a 108-kbp bla NDM-1-positive plasmid.

scholarly journals Characteristics and diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with drug-resistant clinical isolates of Acinetobacter baumannii

2020 ◽  
Author(s):  
Bahare Salehi ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami
Keyword(s):  
Cell Division ◽  
Acinetobacter Baumannii ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Regulatory Genes ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Broth Microdilution Method ◽  
Rnd Efflux Pumps ◽  
Multiple Antibiotics

Abstract Background: This study aimed to characterize the regulation and expression of three putative resistance-nodulation-cell division (RND)-type efflux systems and their contribution to multidrug efflux in clinical isolates of Acinetobacter baumannii. Methods: Antimicrobial susceptibility testing (AST) of 95 A. baumannii isolates was determined by Kirby-Bauer disk diffusion for 18 antibiotics and minimum inhibitory concentration (MIC) of colistin was determined by broth microdilution method. Moreover, MIC of five classes of antibiotics was assessed using E-test strips in the presence and absence of phenylalanine-arginine beta-naphthylamide (PAβN). Regulatory genes of RND efflux pumps (AdeRS, AdeL, AdeN and BaeSR) were subjected to sequencing. The relative expression of adeB. adeG and adeJ genes was determined by quantitative real-time PCR (RT-PCR).Results: Overall, majority of isolates (93%) were extensively drug-resistant (XDR). In the phenotypic assay, efflux pump activity was observed in 40% of isolates against multiple antibiotics mainly tigecycline, but not to imipenem. Several amino acid substitutions were detected in the regulatory genes; except in AdeN. Of note, G186V in AdeS were found to be associated with overexpression of their relative efflux pumps. No insertion sequences (ISs) were detected. Conclusions: Our findings outline the role of RND efflux pumps in resistance of A. baumannii against multiple antibiotics particularly tigecycline, and point out importance of a variety of single mutations in the corresponding regulatory systems. Even though it has been concluded that multidrug resistance occurs as a result of a complex sets of different resistant mechanisms.

scholarly journals Characteristics and diversity of mutations in regulatory genes of resistance-nodulation-cell division efflux pumps in association with drug-resistant clinical isolates of Acinetobacter baumannii

2020 ◽  
Author(s):  
Bahare Salehi ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami
Keyword(s):  
Cell Division ◽  
Acinetobacter Baumannii ◽  
Efflux Pump ◽  
Efflux Pumps ◽  
Regulatory Genes ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Broth Microdilution Method ◽  
Rnd Efflux Pumps ◽  
Multiple Antibiotics

Abstract Background: This study aimed to characterize the regulation and expression of three putative resistance-nodulation-cell division (RND)-type efflux systems and their contribution to multidrug efflux in clinical isolates of Acinetobacter baumannii.Methods: Antimicrobial susceptibility testing (AST) of 95 A. baumannii isolates was determined by Kirby-Bauer disk diffusion for 18 antibiotics and minimum inhibitory concentration (MIC) of colistin was determined by broth microdilution method. Moreover, MIC of five classes of antibiotics was assessed using E-test strips in the presence and absence of phenylalanine-arginine beta-naphthylamide (PAβN). Regulatory genes of RND efflux pumps (AdeRS, AdeL, AdeN and BaeSR) were subjected to sequencing. The relative expression of adeB. adeG and adeJ genes was determined by quantitative real-time PCR (RT-PCR).Results: Overall, majority of isolates (93%) were extensively drug-resistant (XDR). In the phenotypic assay, efflux pump activity was observed in 40% of isolates against multiple antibiotics mainly tigecycline, but not to imipenem. Several amino acid substitutions were detected in the regulatory genes; except in AdeN. Of note, G186V in AdeS were found to be associated with overexpression of their relative efflux pumps. No insertion sequences (ISs) were detected.Conclusions: Our findings outline the role of RND efflux pumps in resistance of A. baumannii against multiple antibiotics particularly tigecycline, and point out importance of a variety of single mutations in the corresponding regulatory systems. Even though it has been concluded that multidrug resistance occurs as a result of a complex sets of different resistant mechanisms.

scholarly journals Complete Genome Sequence of the Clinical Strain Acinetobacter baumannii R2090 Carrying the Chromosomally Encoded Metallo-β-Lactamase Gene bla NDM-1

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Thomas Krahn ◽  
Daniel Wibberg ◽  
Irena Maus ◽  
Anika Winkler ◽  
Patrice Nordmann ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Clinical Strain ◽  
Human Pathogen ◽  
Lactamase Gene

Acinetobacter baumannii is an emerging human pathogen causing nosocomial and community-acquired infections. Here, we present the complete genome sequence of the clinical A. baumannii strain R2090 carrying the metallo-β-lactamase gene bla NDM-1 in its chromosome within the transposon Tn 125 .

scholarly journals First Identification and Characterization of an AdeABC-Like Efflux Pump inAcinetobacterGenomospecies 13TU

2011 ◽  
Vol 55 (3) ◽  
pp. 1285-1286 ◽  
Author(s):  
Ignasi Roca ◽  
Paula Espinal ◽  
Sara Martí ◽  
Jordi Vila
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Clinical Isolate ◽  
Nosocomial Infections ◽  
Efflux Pump ◽  
Hospital Setting ◽  
Multidrug Resistant ◽  
Identification Techniques ◽  
Identification And Characterization

ABSTRACTNon-Acinetobacter baumanniispp. are emerging among clinicalAcinetobacterisolates causing nosocomial infections, and some (such as genomospecies 13TU) appear to be multidrug resistant. The prevalence of non-Acinetobacter baumanniispp. in the hospital setting is likely understated due to poor identification techniques. We report the first identification of an AdeABC-type efflux pump in anAcinetobactergenomospecies 13TU clinical isolate, its contribution to multidrug resistance, and the coexistence of three Ade-type efflux pumps in this strain.

Multidrug Resistance Related to Biofilm Formation in Acinetobacter baumannii and Klebsiella pneumoniae Clinical Strains from Different Pulsotypes

2016 ◽  
Vol 72 (5) ◽  
pp. 617-627 ◽  
Author(s):  
Paola Amaral de Campos ◽  
Sabrina Royer ◽  
Deivid William da Fonseca Batistão ◽  
Bruna Fuga Araújo ◽  
Lícia Ludendorff Queiroz ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Clinical Strains
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